Preparation of Monascus-fermented ginkgo seeds: optimization of fermentation parameters and evaluation of bioactivity

Wu, Zhihao; Wen, Ming‐Shien; Ma, Xuelei; Fan, Gongjian; Wu, Caie; Li, Tingting; Xie, Chunyan; Shen, Dongbei

doi:10.1007/s10068-022-01078-z

Cited by 6 publications

(2 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PB design is able to estimate the key effects of factors with a minimum number of trials in order to quickly and efficiently select the most significantly influenced factors from the many factors examined for the next step of the test. Referring to Tao et al [24] and Wu et al [25], a PB test protocol was developed using Design-Expert 10 software based on the results of the single-factor optimization test, with one low level and one high level for each variable, and three replications were set for each group of tests. The inhibition rate of strain LYMC-3 was used as the response value to screen for factors that had significant effects on the mycelial growth of S. sapinea.…”

Section: Plackett-burman (Pb) Trialmentioning

confidence: 99%

Optimization of Fermentation Conditions for Bacillus pumilus LYMC-3 to Antagonize Sphaeropsis sapinea

et al. 2023

View full text Add to dashboard Cite

Bacillus pumilus is widely used as a biocontrol agent. To further develop the biological control potential of B. pumilus LYMC-3 against pine blight, a statistical experimental design was used to optimize a liquid medium using low-cost substrates to improve its antagonistic activity against Sphaeropsis sapinea. Through the plate antagonism test and greenhouse control effect test, this study determines the antifungal effect of strain LYMC-3 against S. sapinea and pine blight. Moreover, response surface optimization methodologies were used to systematically optimize the medium composition and culture conditions of the LYMC-3 strain. The plate antagonism test showed that the inhibition rate of LYMC-3 fermentation filtrate (diluted 5-fold) was 66.09%. The greenhouse control effect test showed that the control effect of its fermentation filtrate on shoot blight reached 89.99%. The response surface optimization test ultimately determined that a higher inhibition rate can be achieved under these conditions: the optimal medium components were 7.2 g/L glucose, 15 g/L peptone, and 7.1 g/L magnesium sulfate; the optimal culture conditions were 52% liquid volume, 28 °C culture temperature, an initial pH of 7, and 1% bacterial inoculation volume. Under the optimized system, the five-fold diluted LYMC-3 fermentation filtrate inhibition rate against S. sapinea was 81.23%, which was 15.84% higher than that before optimization. Meanwhile, optimize the selection of lower-cost and more commonly used glucose instead of beef paste as the carbon source for the culture medium, and choose cheaper magnesium sulfate instead of sodium chloride as the nitrogen source.

show abstract

Section: Plackett-burman (Pb) Trialmentioning

confidence: 99%

Optimization of Fermentation Conditions for Bacillus pumilus LYMC-3 to Antagonize Sphaeropsis sapinea

et al. 2023

View full text Add to dashboard Cite

show abstract

“…As one of the important by-products from ammonia-based flue gas desulfurization processes, ammonium sulphate is widely used in agriculture, fermentation, mineral leaching and other industries. [1][2][3] However, in the actual crystallization process of ammonium sulphate, there are challenges such as low crystallization rate, unstable nucleation, and poor crystal quality, 4 leading to fine grains and high agglomerated crystalline products with poor fluidity. 5 Therefore, in-depth understanding of the crystallization process contributes to the solution of the above problems and develops an efficient utilization of ammonium sulphate.…”

Section: Introductionmentioning

confidence: 99%

Prediction of metastable zone widths of ammonium sulphate: modification of the nucleation potential model in an electrolyte system

Xue

Yan

et al. 2022

CrystEngComm

View full text Add to dashboard Cite

show abstract

Sequential fermentation of Ginkgo biloba seeds by Bacillus subtilis natto and Lactobacillus plantarum enhanced nutrition, flavor and lipid‐lowering activity

Bai,

Fang,

Jiang

et al. 2024

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUNDGinkgo biloba seeds (GBS) are rich in flavonoids, proteins and reducing sugar, and have been consumed as food and medicinal nuts for thousands of years. However, the presence of ginkgotoxins and their poor palatability limit people's consumption of them.RESULTSThis study used solid‐state fermentation with Bacillus subtilis natto and Lactobacillus plantarum to enhance the safety and benefits of GBS. Optimized fermentation conditions increased the content of beneficial components like total flavonoids, soluble protein and reducing sugar while eliminating unpleasant odors (isoamyl aldehyde and hexanal) and reducing the toxin 4′‐O‐methylpyridoxine by 91.17%. Fermentation of GBS powder can significantly enhance its anti‐inflammatory and antioxidant activities in vitro (P < 0.001). Furthermore, it exhibits a dose‐dependent effect within a certain concentration range. Mixed fermentation (FBnLp) was evaluated for its effects on obesity and metabolic syndrome in mice fed a high‐fat diet. FBnLp significantly reduced body and liver weight gain, prevented dyslipidemia and decreased inflammatory and oxidative stress compared to unfermented GBS. Histological analysis showed that FBnLp improved liver health by reducing fat accumulation and preventing non‐alcoholic fatty liver disease. Meanwhile, it was found that feeding FBnLp increased the expression of CPT‐1α, which regulates energy expenditure and fat breakdown, and downregulated the expression of SREBP‐1c, FAS and ACC, which regulate fat synthesis.CONCLUSIONThis research provides new insights and technological support for the application and development of FBnLp as a functional product, addressing key issues in its use and industry growth. © 2024 Society of Chemical Industry.

show abstract

Preparation of Monascus-fermented ginkgo seeds: optimization of fermentation parameters and evaluation of bioactivity

Cited by 6 publications

References 34 publications

Optimization of Fermentation Conditions for Bacillus pumilus LYMC-3 to Antagonize Sphaeropsis sapinea

Optimization of Fermentation Conditions for Bacillus pumilus LYMC-3 to Antagonize Sphaeropsis sapinea

Prediction of metastable zone widths of ammonium sulphate: modification of the nucleation potential model in an electrolyte system

Sequential fermentation of Ginkgo biloba seeds by Bacillus subtilis natto and Lactobacillus plantarum enhanced nutrition, flavor and lipid‐lowering activity

Contact Info

Product

Resources

About