Preparation of monoclonal antibodies against human thyrotropin suitable for diagnostic systems and their use in clinical practice

Abstract

Original methods are presented for immunizing mice with preparations of human thyrotropin and for testing primary clones of producer hybridomas. Twelve monoclonal antibodies specific to six different regions of the antigen are obtained. A test system for enzyme immunoassay is developed on the basis of two antibodies, providing for detection of hypo-and hyperthyreoidism in clinical practice.