Preparation of monodisperse PEG hydrogel composite microspheres via microfluidic chip with rounded channels

Yu, Bing; Cong, Hailin; Liu, Xuesong; Ren, Yumin; Wang, Jilei; Zhang, Lixin; Tang, Jianguo; Ma, Yurong; Akasaka, Takeshi

doi:10.1088/0960-1317/23/9/095016

Cited by 13 publications

(7 citation statements)

References 37 publications

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“…The tendency of the MPs´ diameters regarding Qc is that the diameter of the MPs is inversely proportional to the increase in the flow of Qc; this is represented in Equation (3), and these results are consistent with those of other authors [ 33 , 34 , 35 , 42 , 43 , 49 ]. However, with this study, we can observe that Qc is the most important factor in relation to the diameter of MPs .…”

Section: Resultssupporting

confidence: 91%

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Generation of Photopolymerized Microparticles Based on PEGDA Hydrogel Using T-Junction Microfluidic Devices: Effect of the Flow Rates

et al. 2023

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The formation of microparticles (MPs) of biocompatible and biodegradable hydrogels such as polyethylene glycol diacrylate (PEGDA) utilizing microfluidic devices is an attractive option for entrapment and encapsulation of active principles and microorganisms. Our research group has presented in previous studies a formulation to produce these hydrogels with adequate physical and mechanical characteristics for their use in the formation of MPs. In this work, hydrogel MPs are formed based on PEGDA using a microfluidic device with a T-junction design, and the MPs become hydrogel through a system of photopolymerization. The diameters of the MPs are evaluated as a function of the hydrodynamic condition flow rates of the continuous (Qc) and disperse (Qd) phases, measured by optical microscopy, and characterized through scanning electron microscopy. As a result, the following behavior is found: the diameter is inversely proportional to the increase in flow in the continuous phase (Qc), and it has a significant statistical effect that is greater than that in the flow of the disperse phase (Qd). While the diameter of the MPs is proportional to Qd, it does not have a significant statistical effect on the intervals of flow studied. Additionally, the MPs’ polydispersity index (PDI) was measured for each experimental hydrodynamic condition, and all values were smaller than 0.05, indicating high homogeneity in the MPs. The microparticles have the potential to entrap pharmaceuticals and microorganisms, with possible pharmacological and bioremediation applications.

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Section: Resultssupporting

confidence: 91%

“…In Table 1 , it can be observed that none of these studies have used eosine Y as a photo-initiator to start the curing process [ 38 , 40 , 41 , 42 , 43 , 44 ]. In this work, eosin Y is used as the photo-initiator, which needs light with a wavelength of about 520 nm, or visible light [ 2 , 45 , 46 ].…”

Section: Introductionmentioning

confidence: 99%

Generation of Photopolymerized Microparticles Based on PEGDA Hydrogel Using T-Junction Microfluidic Devices: Effect of the Flow Rates

et al. 2023

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“…The experimental results showed that microfluidic is effective way for accurate control of synthetic titania colloidal particles. Droplet microfluidics is also used to synthesize polymer particles by means of polymerization with an online ultraviolet (UV) curing lamp . Additionally, polymer colloidal particles (e.g., polystyrene, polymethyl methacrylate) can also be obtained from droplet microfluidic chips with optimized conditions.…”

Section: Microfluidic Classification and Synthesis Of Colloidal Partimentioning

confidence: 99%

Colloidal Crystals from Microfluidics

Bian

Sun

Cai

et al. 2019

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Colloidal crystals are of great interest to researchers because of their excellent optical properties and broad applications in barcodes, sensors, displays, drug delivery, and other fields. Therefore, the preparation of high quality colloidal crystals in large quantities with high speed is worth investigating. After decades of development, microfluidics have been developed that provide new choices for many fields, especially for the generation of functional materials in microscale. Through the design of microfluidic chips, colloidal crystals can be prepared controllably with the advantages of fast speed and low cost. In this Review, research progress on colloidal crystals from microfluidics is discussed. After summarizing the classifications, the generation of colloidal crystals from microfluidics is discussed, including basic colloidal particles preparation, and their assembly inside or outside of microfluidic devices. Then, applications of the achieved colloidal crystals from microfluidics are illustrated. Finally, the future development and prospects of microfluidic‐based colloidal crystals are summarized.

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“…There have been several methods reported for producing microparticles. For instance, the monodispersed microfluidic approach is commonly used to create collagen microspheres [24][25][26][27][28][29][30]. The high throughput production is the main advantage of this approach; however, it has not been successful in biomedical applications because oil phase contamination inhibits cellular function in collagen microparticles.…”

Section: Introductionmentioning

confidence: 99%

Microencapsulation of beta cells in collagen micro-disks via circular pneumatically actuated soft micro-mold (cPASMO) device

Huang

Saha

et al. 2018

Biomed. Phys. Eng. Express

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The artificial pancreas provides a method for automatically managing insulin concentrations in patients with Type I diabetes. One strategy for fabricating an artificial pancreas is the encapsulation of beta cells in a collagen matrix, which can provide mechanical support. In addition, encapsulation of beta cells in small volumes and with high surface areas allows them to quickly sense glucose and secrete insulin for regulation. Currently, there is no such fabrication technique of encapsulating beta cells in a collagen matrix, due to their inherent fragility. Herein, we develop a novel technique called circular Pneumatic Actuated Soft Micromold (cPASMO) to produce collagen microdisks for encapsulating beta cells. cPASMO consists of a particle template, pneumatic channel, and buffer layer to control deformation. The actuation mechanism is simply to deform the template by increasing the inner pressure inside the channel. This deformation in the template generates a gap between the collagen microdisks and mold that can extract the collagen microdisks via buoyancy force. Thus, they can be released from their templates without damage. The position of the air channel determines the uniformity of the template deformation that leads to the high collagen microdisk extraction rate. Compared to the previous design (i.e., linear air channel), the extraction rate is four times faster. We demonstrated that beta cells encapsulated in collagen micro disks by a cPASMO device exhibit a biological function of rapidly sensing glucose and secreting insulin for regulation. The concentration of the secreted insulin from the encapsulated beta cells is three to six times higher than that of suspended cells. Also, smaller collagen micro disks release higher concentrations of insulin than those that are larger because the larger spaces between beta cells can degrade cellular communication. These breakthroughs in producing collagen microdisks will push the boundaries of treatment for Type I diabetic patients.

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Preparation of monodisperse PEG hydrogel composite microspheres via microfluidic chip with rounded channels

Cited by 13 publications

References 37 publications

Generation of Photopolymerized Microparticles Based on PEGDA Hydrogel Using T-Junction Microfluidic Devices: Effect of the Flow Rates

Generation of Photopolymerized Microparticles Based on PEGDA Hydrogel Using T-Junction Microfluidic Devices: Effect of the Flow Rates

Colloidal Crystals from Microfluidics

Microencapsulation of beta cells in collagen micro-disks via circular pneumatically actuated soft micro-mold (cPASMO) device

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