Preparation of natural three-dimensional goat kidney scaffold for the development of bioartificial organ

Vishwakarma, Sandeep Kumar; Bhavani, P.G.; Bardia, Avinash; Abkari, Anand; Murthy, G S N; Venkateshwarulu, J; Khan, Anousha A.

doi:10.4103/0971-4065.133008

Cited by 20 publications

(8 citation statements)

References 9 publications

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“…in 2009 . Subsequently, different decellularization protocols were applied to kidneys from different species, such as mouse, rat, goat, pig, monkey and human . Decellularization of whole kidneys is generally performed via the renal artery.…”

Section: Kidneymentioning

confidence: 99%

Strategies based on organ decellularization and recellularization

et al. 2019

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Summary Transplantation is the only curative treatment option available for patients suffering from end‐stage organ failure, improving their quality of life and long‐term survival. However, because of organ scarcity, only a small number of these patients actually benefit from transplantation. Alternative treatment options are needed to address this problem. The technique of whole‐organ decellularization and recellularization has attracted increasing attention in the last decade. Decellularization includes the removal of all cellular components from an organ, while simultaneously preserving the micro and macro anatomy of the extracellular matrix. These bioscaffolds are subsequently repopulated with patient‐derived cells, thus constructing a personalized neo‐organ and ideally eliminating the need for immunosuppression. However, crucial problems have not yet been satisfyingly addressed and remain to be resolved, such as organ and cell sources. In this review, we focus on the actual state of organ de‐ and recellularization, as well as the problems and future challenges.

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Section: Kidneymentioning

confidence: 99%

Strategies based on organ decellularization and recellularization

et al. 2019

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“…The kidney was kept suspended in the beaker, and intravenous connector was connected to the vein cannula (syringe needle) [ Figure 2 ]. [ 11 ]…”

Section: Methodsmentioning

confidence: 99%

“…The decellularized kidney capsule was further injected and perfused with 1% trypan blue for localization of the kidney scaffold architecture including venules, arterioles and smaller veins and arteries. [ 10 ] To distinguish between the trypan blue dye perfused and nonperfused kidney scaffold, we performed some ImageJ software analysis[ 11 ] and further observed the comparable minute details for the organization of renal vasculature architecture and texture.…”

Section: Methodsmentioning

confidence: 99%

Development of an experimental model of a decellularized kidney scaffold by perfusion mode and analyzing the three-dimensional extracellular matrix architecture by edge detection method

2018

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Renal transplant is treatment of choice for the patients with end stage renal disease. The kidney transplants are expensive and there are risks of immunological and infectious complications. We planned to develop an in vitro decellularized kidney scaffold model using sheep kidney. Kidney decellularization was carried out by perfusing chemical detergents such as sodium dodecyl sulfate (SDS), SDS and trypsin, and SDS and ethylenediaminetetraacetic acid solvent solution. Complete kidney was decellularized in 5 days by perfusing various chemical detergents in time-dependent intervals. Histological finding revealed the complete removal of cellular material in various regions of renal corpuscle, distal convoluted tubules, other cortex and medulla region. Details of interlobular veins and arteries were seen through naked eyes after trypan blue dye injection. We used edge detection technique for developing a three-dimensional (3-D) image (Image J software) for nephrological vasculature constructed of decellularized kidney scaffold specimen. This technique opens a gateway for the whole organ decellularization by perfusion technology and further imaging of its 3-D extracellular matrix texture by edge detection technique software.

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“…Kidney decellularization success is often reliant upon the organ harvest, which must have occurred prior to proteolysis (4-6 h post-mortem) [41], and the age of the kidney has also been shown to have an effect on cellular repopulation [187]. Xeno matrices, including the use of porcine tissues, have also been investigated to reduce some of these barriers [183,188,189], since the organ size is comparable to humans [190][191][192][193] and porcine matrices are able to promote the adhesion, survival, and maintenance of human cells [180,[194][195][196]. However, for decellularization to be a viable starting point, there is a need to standardise decellularization processes and consider the manufacturing challenges including scalability, sterilisation, and preservation.…”

Section: Tissue Decellularizationmentioning

confidence: 99%

A critical review of current progress in 3D kidney biomanufacturing: advances, challenges, and recommendations

2019

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The widening gap between organ availability and need is resulting in a worldwide crisis, particularly concerning kidney transplantation. Regenerative medicine options are becoming increasingly advanced and are taking advantage of progress in novel manufacturing techniques, including 3D bioprinting, to deliver potentially viable alternatives. Cell-integrated and wearable artificial kidneys aim to create convenient and efficient systems of filtration and restore elements of immunoregulatory function. Whilst preliminary clinical trials demonstrated promise, manufacturing and trial design issues and identification of suitable and sustainable cell sources have shown that more development is required for market progression. Tissue engineering and advances in biomanufacturing techniques offer potential solutions for organ shortages; however, due to the complex kidney structure, previous attempts have fallen short. With the recent development and progression of 3D bioprinting, cell positioning and resolution of material deposition in organ manufacture have never seen greater control. Cell sources for constructing kidney building blocks and populating both biologic and artificial scaffolds and matrices have been identified, but in vitro culturing and/or differentiation, in addition to maintaining phenotype and viability during and after lengthy and immature manufacturing processes, presents additional problems. For all techniques, significant process barriers, clinical pathway identification for translation of models to humans, scaffold material availability, and long-term biocompatibility need to be addressed prior to clinical realisation.

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Preparation of natural three-dimensional goat kidney scaffold for the development of bioartificial organ

Cited by 20 publications

References 9 publications

Strategies based on organ decellularization and recellularization

Strategies based on organ decellularization and recellularization

Development of an experimental model of a decellularized kidney scaffold by perfusion mode and analyzing the three-dimensional extracellular matrix architecture by edge detection method

A critical review of current progress in 3D kidney biomanufacturing: advances, challenges, and recommendations

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