Preparation of new synthons for designing polyfunctional chelating agents

Synthesis of magnetic nanoparticles with an IDA or TED modified surface for purification and immobilization of poly-histidine tagged proteins

“…The synthesis of APTES-ECH-IDA was based on previous reports. [39][40][41] Typically, 1.0 g IDA was dissolved in 25 mL deionized water containing 1.2 g NaOH. Then, 1.2 mL epichlorohydrin was added and reacted at 40 C for 4 hours.…”