Preparation of Non-Toxic Urine Fractions for Assay of Male Hormone by the Female Bitterling Test

Abstract

hundred eggs on a bit of cheese-cloth. In the case of Papilio ajax, a breeding cage was employed and a sprig of wild parsnip was placed in its best lighted corner. The result was excellent. In order to rear larvae, the bits of leaves with attached eggs were placed on fresh food plant in tin cans covered with pieces of glass. The plant in this case was also kept in a curved-necked bottle. This type of tight container retained the moisture, which is absolutely essential for small larvae. However, they were soon transferred to large breeding cages.