Preparation of novel RGD-conjugated thermosensitive mPEG-PCL composite hydrogels and in vitro investigation of their impacts on adhesion-dependent cellular behavior

Kim, Hyun Joo; You, Su Jung; Yang, Dae Hyeok; Chun, Heung Jae; Kim, Moon Suk

doi:10.1016/j.jiec.2020.01.001

Cited by 16 publications

(9 citation statements)

References 24 publications

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“…In addition to in vivo tracking of nanoparticle-labeled BMSCs, we also traced the GFP-transfected BMSCs seeded in cardiac patch following in vivo transplantation [ 22 ]. And the expressions of myocardial cell-specific markers were confirmed using immunofluorescence staining in order to investigate whether the BMSCs migrated to the ischemic region and were associated with the therapeutic and recovery effects of myocardial regeneration (Fig.…”

Section: Resultsmentioning

confidence: 99%

Stem cell laden nano and micro collagen/PLGA bimodal fibrous patches for myocardial regeneration

et al. 2022

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Background Although the use of cardiac patches is still controversial, cardiac patch has the significance in the field of the tissue engineered cardiac regeneration because it overcomes several shortcomings of intra-myocardial injection by providing a template for cells to form a cohesive sheet. So far, fibrous scaffolds fabricated using electrospinning technique have been increasingly explored for preparation of cardiac patches. One of the problems with the use of electrospinning is that nanofibrous structures hardly allow the infiltration of cells for development of 3D tissue construct. In this respect, we have prepared novel bi-modal electrospun scaffolds as a feasible strategy to address the challenges in cardiac tissue engineering . Methods Nano/micro bimodal composite fibrous patch composed of collagen and poly (D, L-lactic-co-glycolic acid) (Col/PLGA) was fabricated using an independent nozzle control multi-electrospinning apparatus, and its feasibility as the stem cell laden cardiac patch was systemically investigated. Results Nano/micro bimodal distributions of Col/PLGA patches without beaded fibers were obtained in the range of the 4-6% collagen concentration. The poor mechanical properties of collagen and the hydrophobic property of PLGA were improved by co-electrospinning. In vitro experiments using bone marrow-derived mesenchymal stem cells (BMSCs) revealed that Col/PLGA showed improved cyto-compatibility and proliferation capacity compared to PLGA, and their extent increased with increase in collagen content. The results of tracing nanoparticle-labeled as well as GFP transfected BMSCs strongly support that Col/PLGA possesses the long-term stem cells retention capability, thereby allowing stem cells to directly function as myocardial and vascular endothelial cells or to secrete the recovery factors, which in turn leads to improved heart function proved by histological and echocardiographic findings. Conclusion Col/PLGA bimodal cardiac patch could significantly attenuate cardiac remodeling and fully recover the cardiac function, as a consequence of their potent long term stem cell engraftment capability.

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Section: Resultsmentioning

confidence: 99%

Stem cell laden nano and micro collagen/PLGA bimodal fibrous patches for myocardial regeneration

et al. 2022

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“…In addition, the in situ forming MPEG-PCL hydrogel has shown excellent retention capabilities for mesenchymal stem cells (MSCs) 21 – 23 . A drawback of MPEG-PCL is the lack of a cell recognition site for focal adhesion to orchestrate various aspects of cellular behaviors for development and maintenance of tissues 22 . The tripeptide RGD is a representative motif that is highly effective at promoting attachment of various cell types 7 , 18 , 22 .…”

Section: Introductionmentioning

confidence: 99%

“…A drawback of MPEG-PCL is the lack of a cell recognition site for focal adhesion to orchestrate various aspects of cellular behaviors for development and maintenance of tissues 22 . The tripeptide RGD is a representative motif that is highly effective at promoting attachment of various cell types 7 , 18 , 22 . Other cell-adhesive peptide sequences, such as YIGSR, IKVAV, and GFOGER, have been identified from native ECM components.…”

Section: Introductionmentioning

confidence: 99%

In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration

Yang

You

et al. 2023

npj Regen Med

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The collagen-mimetic peptide GFOGER possesses the chondrogenic potential and has been used as a cell adhesion peptide or chondrogenic inducer. Here, we prepared an injectable in situ forming composite hydrogel system comprising methoxy polyethylene glycol-b-polycaprolactone (MPEG-PCL) and GFOGER-conjugated PEG-PCL (GFOGER-PEG-PCL) with various GFOGER concentrations based on our recently patented technology. The conjugation of GFOGER to PEG-PCL was confirmed by 1H NMR, and the particle size distribution and rheological properties for the sol-gel transition behavior of the samples with respect to the GFOGER content were evaluated systemically. In vitro experiments using rat bone marrow-derived mesenchymal stem cells (BMSCs) revealed that the GFOGER-PEG-PCL hydrogel significantly enhanced expression of integrins (β1, α2, and α11), increased expression of FAK, and induced downstream signaling of ERK and p38. Overexpression of chondrogenic markers suggested that BMSCs have the potential to differentiate into chondrogenic lineages within GFOGER-PEG-PCL samples. In vivo studies using a rat osteochondral defect model revealed that transplanted BMSCs with GFOGER0.8-PEG-PCL survived at the defect with strong chondrogenic expression after 4 weeks. The stem cell-laden GFOGER0.8-PEG-PCL hydrogel produced remarkable osteochondral regeneration at 8 weeks of transplantation, as determined by histological findings and micro-CT analysis. The histomorphological score in the GFOGER0.8-PEG-PCL + BMSCs group was ~1.7-, 2.6-, and 5.3-fold higher than that in the GFOGER0.8-PEG-PCL, MPEG-PCL, and defect groups, respectively. Taken together, these results provide an important platform for further advanced GFOGER-based stem cell research for osteochondral repair.

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“…Hydrogel mimics salient components of native extracellular matrices (ECM) and has similar mechanical properties to those of many soft tissues [ 7 , 8 , 9 ]. The application of hydrogels has proven to be effective in a range of cell culture applications to a delivery system [ 10 , 11 , 12 ]. Advanced hydrogel-based biomaterials are continuously proposed and studied.…”

Section: Introductionmentioning

confidence: 99%

“…Among various types of hydrogels, thermosensitive hydrogels possess great potential for a wide range of TE applications [ 12 , 13 , 14 , 15 ]. The sol–gel transition of thermosensitive hydrogels can appear at a physiological temperature (~37 °C).…”

Section: Introductionmentioning

confidence: 99%

Pluronic F-127/Silk Fibroin for Enhanced Mechanical Property and Sustained Release Drug for Tissue Engineering Biomaterial

et al. 2021

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Herein, an injectable thermosensitive hydrogel was developed for a drug and cellular delivery system. The composite was prepared by facile physical mixing of pluronic F-127 (PF) and silk fibroin (SF) in an aqueous solution. The chemical structure, transparency, viscosity, injectability, degradation kinetic, cumulative release of dexamethasone (Dex), a type of corticosteroid drug, and size distribution of the fabricated hydrogels were characterized. Cytotoxicity of the hydrogels was also studied to verify the biocompatibility of the hydrogels. The addition of a proper amount of SF to PF not only improved the mechanical strength but also decreased the degradation rate which improved the fast rate release of hydrophobic drugs. The cytotoxicity of the hydrogel decreased when SF was added to PF in a proper amount. Overall, the results confirm that the composite of PF and SF can be a promising cell and drug delivery system for future application in tissue engineering and regenerative medicine.

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Preparation of novel RGD-conjugated thermosensitive mPEG-PCL composite hydrogels and in vitro investigation of their impacts on adhesion-dependent cellular behavior

Cited by 16 publications

References 24 publications

Stem cell laden nano and micro collagen/PLGA bimodal fibrous patches for myocardial regeneration

Stem cell laden nano and micro collagen/PLGA bimodal fibrous patches for myocardial regeneration

In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration

Pluronic F-127/Silk Fibroin for Enhanced Mechanical Property and Sustained Release Drug for Tissue Engineering Biomaterial

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