Preparation of Oil Palm Empty Fruit Bunch Hydrolysate

Akita, Hironaga; Yusoff, Mohd Zulkhairi Mohd; Fujimoto, Shinji

doi:10.3390/fermentation7020081

Cited by 10 publications

(5 citation statements)

References 23 publications

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“…A similar increasing trend was observed for cellobiose, arabinose, galactose, and mannose after SCW pre-treatment. Likewise, Akita et al (2021) reported an increase in the concentration of glucose, cellobiose and mannose in the liquid fraction of pretreated EFB along with the increasing temperature. Meanwhile, xylose composition was not detected at a lower temperature.…”

Section: P R E S Smentioning

confidence: 82%

Evaluating the Effect of Low and High Temperature Mode of Subcritical Water Pre-Treated Empty Fruit Bunches on Co-Digestion Performance and Kinetic Study for Methane Production

HAMZAH

2023

JOPR

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Anaerobic digestion of oil palm empty fruit bunches (EFB) is considered an effective method for non-renewable energy substitution through biogas production. However, lignocellulosic recalcitrance structure of EFB is one of the main difficulties in achieving high biogas production for anaerobic co-digestion with palm oil mill effluents (POME). In this study, EFB was pre-treated with subcritical water (SCW) at low (120°C) and high (180°C) temperatures for 10 to 30 min to enhance biogas production. The characteristics of EFB after SCW pre-treatment were evaluated to identify changes in physicochemical characteristics. The combination pretreatment of 180°C for 10 min with 546.18 mL g -1 volatile solid (VS) biogas yield and 421.41 mL CH 4 g -1 VS methane yield revealed the highest biogas production. Meanwhile, co-digestion of SCW pre-treated EFB with POME led to a removal of more than 66% VS. The sugars released were analysed in liquid fraction of SCW pre-treated EFB where glucose, xylose, cellobiose, mannose and galactose were detected. Notably, kinetic study of biogas production of pre-treated EFB using modified Gompertz model revealed that pre-treatment improved the lag phase, and the highest biogas production rate was observed at 19.80 mL g -1 VS. day. In conclusion, co-digestion of EFB with POME for methane production can be improved with the use of SCW pre-treatment.

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Section: P R E S Smentioning

confidence: 82%

Evaluating the Effect of Low and High Temperature Mode of Subcritical Water Pre-Treated Empty Fruit Bunches on Co-Digestion Performance and Kinetic Study for Methane Production

HAMZAH

2023

JOPR

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“…for 3 days at 50 • C in an incubator (SLI-700; EYELA, Tokyo, Japan), the dried samples were milled using a cutter mill (ABSOLUTE3; Osaka Chemical, Osaka, Japan), yielding a powder (particle size: 100-200 µm). On the basis of the findings of our previous study [21], the hydrothermal treatment of the powder was carried out. Briefly, 10 g of the powder was placed in an autoclave with 100 mL water for 30 min at 130 • C, and the resulting hydrothermally treated samples were used as the initial raw material for enzymatic hydrolysis.…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

Simultaneous Saccharification and Fermentation for Isobutanol Production from Banana Peel

Akita,

Shibata,

Komoriya

et al. 2024

Fermentation

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Each year, near 40 million tons of banana peels are discarded around the world. This plant biomass could potentially be utilized for energy production. Simultaneous saccharification and fermentation (SSF) is an effective method for producing biofuels from plant biomasses. Since SSF with enzymatic hydrolysis and fermentation are performed simultaneously in the same reactor, the production process is simpler than most existing methods. Here, we describe isobutanol production using SSF with hydrothermally treated banana peel samples and an Escherichia coli strain able to utilize glucose and xylose to produce isobutanol. To enhance the glucose and xylose concentrations, the reaction conditions for the enzymatic hydrolysis of plant biomass using two kinds of saccharification enzymes were optimized, including the enzyme unit ratio, reaction temperature and sample gram. When the optimized conditions for enzymatic hydrolysis were applied to SSF, the glucose and xylose produced from the hydrothermally treated samples were consumed, producing isobutanol. Moreover, the isobutanol concentration increased with an increasing initial culture pH, reaching 1.27 g/L at pH 6.5, which was consistent with the optimal initial culture pH for isobutanol production by this E. coli strain. Taken together, these results indicate that the established method is potentially useful for industrial isobutanol production.

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“…Humic acid producing microorganisms have been isolated from soil, the marine environment waste volatile substances (Muthumariet al, 2016), palm-oil mill effluent, (Jagtap andChobade, 2015). The present study addresses the dual challenges of agricultural waste management and soil health improvement by focusing on the isolation, identification, and screening of humic acid-producing fungi from the soil environment of Oil Palm EFB waste (Akita et al, 2021).…”

Section: E-issn: 2814 -1822; P-issn: 2616 -0668mentioning

confidence: 99%

Isolation, Identification and Screening of Humic Acid Producing Fungi from Soil Environment of Oil palm (Elaeisguineensis) Associated with Empty Fruit Bunches

Kawata,

Omojasola,

Ajiboye

et al. 2023

UJMR

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The demand for sustainable and sustainable biotechnological processes has developed as a result of a growing interest in converting agricultural waste into valuable products. In this study, fungal strainswere isolated from soil environment ofoil palm empty fruit bunchesandscreened for humic acid production. In Osun state, Nigeria, soil sample was taken from a local palm oil mill. Different colonies of fungi were obtained for further research using the serial dilution plating procedure. Plate screening was used to evaluate the fungal biomass as well as colony diameter of the fungal isolates, and a submerged fermentation test was used to measure the amount of humic acid concentration the isolates produced. Fungal isolates were identified by using molecular methods and morphological analysis. The fungi species Aspergillusniger, Rhizopusstolonifer, and Penicilliumchrysogenum were isolated from the soil environment. When compared to Rhizopusstolonifer, Aspergillusniger grew more superiorly in terms of zone diameter (26.5 1.50 mm) and biomass (2.23 0.16 g), while Penicilliumchrysogenum showed no growth. Throughout the fermentation process, Aspergillusnigeralso produced a greater quantity of humic acid, with concentrations varying from 0.70 mg/l to 2.20 mg/l. Aspergillusniger strain HR18's existence was confirmed by molecular analysis. This experiment proved that Aspergillusniger can efficiently produce humic acid from OPEFB, providing a useful method for waste utilization and sustainable practices.

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Preparation of Oil Palm Empty Fruit Bunch Hydrolysate

Cited by 10 publications

References 23 publications

Evaluating the Effect of Low and High Temperature Mode of Subcritical Water Pre-Treated Empty Fruit Bunches on Co-Digestion Performance and Kinetic Study for Methane Production

Evaluating the Effect of Low and High Temperature Mode of Subcritical Water Pre-Treated Empty Fruit Bunches on Co-Digestion Performance and Kinetic Study for Methane Production

Simultaneous Saccharification and Fermentation for Isobutanol Production from Banana Peel

Isolation, Identification and Screening of Humic Acid Producing Fungi from Soil Environment of Oil palm (Elaeisguineensis) Associated with Empty Fruit Bunches

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