Preparation of one- to four-branch silver nanostructures of various sizes by metallization of hybrid DNA–protein assemblies

Rudiuk, Sergii; Venancio‐Marques, Anna; Hallais, Géraldine; Baigl, Damien

doi:10.1039/c3sm50710f

Cited by 15 publications

(11 citation statements)

References 50 publications

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“…The assembled nanowire had a width of about 25–200 nm and a height of approximately 6.5 nm ( Fig. 1F ), a typical thickness previously reported for nanowire structures 36 37 38 39 . Moreover, we observed many red points at the terminal and conjunction of the nanowire, which may be the complex Λ-RM0627 confined in the G-quadruplex conformation of DNA 24 .…”

Section: Resultssupporting

confidence: 72%

Self-assembly of c-myc DNA promoted by a single enantiomer ruthenium complex as a potential nuclear targeting gene carrier

Mei

Zheng

et al. 2016

Sci Rep

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Gene therapy has long been limited in the clinic, due in part to the lack of safety and efficacy of the gene carrier. Herein, a single enantiomer ruthenium(II) complex, Λ-[Ru(bpy)2(p-BEPIP)](ClO4)2 (Λ-RM0627, bpy = 4,4′-bipyridine, p-BEPIP = 2-(4-phenylacetylenephenyl)imidazole [4,5f][1, 10] phenanthroline), has been synthesized and investigated as a potential gene carrier that targets the nucleus. In this report, it is shown that Λ-RM0627 promotes self-assembly of c-myc DNA to form a nanowire structure. Further studies showed that the nano-assembly of c-myc DNA that induced Λ-RM0627 could be efficiently taken up and enriched in the nuclei of HepG2 cells. After treatment of the nano-assembly of c-myc DNA with Λ-RM0627, over-expression of c-myc in HepG2 cells was observed. In summary, Λ-RM0627 played a key role in the transfer and release of c-myc into cells, which strongly indicates Λ-RM0627 as a potent carrier of c-myc DNA that targets the nucleus of tumor cells.

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Section: Resultssupporting

confidence: 72%

Self-assembly of c-myc DNA promoted by a single enantiomer ruthenium complex as a potential nuclear targeting gene carrier

Mei

Zheng

et al. 2016

Sci Rep

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“…In the absence of strep, bt-T4DNA appeared as threads with an average height of 0.48 ± 0.07 nm, in agreement with previous observations on DNA adsorbed on mica. [35][36][37] In the presence of strep, the bright spots observed in both raspberry and pearlnecklace structures had an average height of 1.6 ± 0.6 nm, a value similar to the height of free streptavidin adsorbed on mica ( Figure S5, Supporting Information), and were thus attributed to streptavidin crosslinks. We finally characterized the thermal stability of IPDGs in the maximal compaction state (raspberry structures, [strep] = 10 × 10 −9 m) by incubating those for 4 h at temperatures ranging from 20 to 65 °C.…”

Section: Resultsmentioning

confidence: 99%

Intramolecularly Protein-Crosslinked DNA Gels: New Biohybrid Nanomaterials with Controllable Size and Catalytic Activity

Zhou

Morel

Rudiuk

et al. 2017

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DNA micro- and nanogels-small-sized hydrogels made of a crosslinked DNA backbone-constitute new promising materials, but their functions have mainly been limited to those brought by DNA. Here a new way is described to prepare sub-micrometer-sized DNA gels of controllable crosslinking density that are able to embed novel functions, such as an enzymatic activity. It consists of using proteins, instead of traditional base-pairing assembly or covalent approaches, to form crosslinks inside individual DNA molecules, resulting in structures referred to as intramolecularly protein-crosslinked DNA gels (IPDGs). It is first shown that the addition of streptavidin to biotinylated T4DNA results in the successful formation of thermally stable IPDGs with a controllable crosslinking density, forming structures ranging from elongated to raspberry-shaped and pearl-necklace-like morphologies. Using reversible DNA condensation strategies, this paper shows that the gels can be reversibly actuated at a low crosslinking density, or further stabilized when they are highly crosslinked. Finally, by using streptavidin-protein conjugates, IPDGs with various enzymes are successfully functionalized. It is demonstrated that the enzymes keep their catalytic activity upon their incorporation into the gels, opening perspectives ranging from biotechnologies (e.g., enzyme manipulation) to nanomedicine (e.g., vectorization).

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“…13) [160,161]. The mean length of the DNA branches upon the deposition of SN on them is reduced from 174-181 to 60-68 nm due to conformational transformations of the DNA macrochains upon their interaction with SN [161].…”

Section: Hybrid Systems With An Organic Polymer Matrixmentioning

confidence: 97%

“…Modification of DNA molecules with biotin imparts high affinity for the protein, streptavidin, while the deposition of SN on streptavidin-DNA self-aggregates (streptavidin may bind up to four DNA macromolecules) permits the preparation of hybrid polymer nanosystems (Fig. 13) [160,161]. The mean length of the DNA branches upon the deposition of SN on them is reduced from 174-181 to 60-68 nm due to conformational transformations of the DNA macrochains upon their interaction with SN [161].…”

Section: Hybrid Systems With An Organic Polymer Matrixmentioning

confidence: 99%

Preparation, Structure, and Properties of Hybrid Polymer Composites Containing Silver Clusters and Nanoparticles

Tolstov

2015

Theor Exp Chem

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The major methods for the preparation of silver-containing polymer composite nanosystems and the formation of their hybrid structure are examined. Special attention is focused on a new class of silver-containing hybrid systems, namely, organic-inorganic coordination polymers, obtained by the self-assembly of silver complexes. The effect of intercomponent interaction on the structure and properties of these hybrid composites was examined.The creation of new improved materials is an important goal of modern chemistry and materials science. Polymer composites with hybrid structure are among such materials and hold interest since they display a combination of properties characteristic for each of the inorganic and organic components [1-3] and, in some cases, unique properties related to the specific nature of their structure [4]. From a modern viewpoint, hybrid organic-inorganic materials are formed by the combination of components, differing in their chemical nature, on a molecular or nano level through formation of chemical bonds ((non)covalent bonds, hydrogen bonding, and donor-acceptor bonds) or weaker physical bonds (van der Waals and electrostatic interactions) [5]. Special attention is given to the stability of the nanodispersed components due to interaction between the nanophase and various stabilizers [6]. The interest in silver-containing hybrid polymer composite materials, including nanocomposites, which, by their very nature, are a priori hybrid systems, is attributed to a set of properties of the matrix itself, which often possesses hybrid structure, properties of silver in ionic and nanodispersed forms, and new properties related to the appearance of hybrid silver-polymer structures [7,8]. Special interest is presently found in bionanocomposites derived from biopolymers and silver nanoparticles (SN), which display specific physicochemical properties, in particular, electrochemical, photochemical, and catalytic properties highly dependent on the dimensions and polydispersion of the nanophase [9, 10] as well as biological activity due to the ionization of the metal atoms in the presence of H 2 O/O 2 and the interaction between the SN surface and functional groups of the various biomolecules [11].In light of the important role of the polymer matrix in formation of the major structural characteristics and properties of silver-containing hybrid systems, we should classify these materials according to their chemical structure.Composites with Silver Nanoparticles (SN) Dispersed in a Polymer Matrix with Hybrid Structure. The major distinguishing feature of such materials is the prior synthesis of the hybrid polymer matrix obtained due to the formation of chemical bonds between the organic and inorganic components with subsequent introduction of SN into the system. The synthesis of the SN may be either in situ or ex situ. 740040-5760/15/5102-0074

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Preparation of one- to four-branch silver nanostructures of various sizes by metallization of hybrid DNA–protein assemblies

Cited by 15 publications

References 50 publications

Self-assembly of c-myc DNA promoted by a single enantiomer ruthenium complex as a potential nuclear targeting gene carrier

Self-assembly of c-myc DNA promoted by a single enantiomer ruthenium complex as a potential nuclear targeting gene carrier

Intramolecularly Protein-Crosslinked DNA Gels: New Biohybrid Nanomaterials with Controllable Size and Catalytic Activity

Preparation, Structure, and Properties of Hybrid Polymer Composites Containing Silver Clusters and Nanoparticles

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