Preparation of Oriented DNA by Wet Spinning.

Rupprecht, A.; Bovallius, Å.; Bergson, Göran; Ehrenberg, L.; Brünvoll, J.; Bunnenberg, E.; Djerassi, Carl; Records, Ruth

doi:10.3891/acta.chem.scand.20-0494

Cited by 109 publications

(54 citation statements)

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“…Oriented films of DNA and poly A⅐poly U were prepared by wet spinning from aqueous solutions 10 and further treated as described in detail previously, 11 and unoriented deposits of AU and MeAU were prepared by drying the aqueous solutions. All the nucleic acids were studied as sodium salts.…”

Section: Methodsmentioning

confidence: 99%

Hydration of biological molecules: Lipids versus nucleic acids

Pohle

Gauger

Dornberger³

et al. 2002

Biopolymers

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We used FTIR spectroscopy to comparatively study the hydration of films prepared from nucleic acids (DNA and double-stranded RNA) and lipids (phosphatidylcholines and phosphatidylethanolamines chosen as the most abundant ones) at room temperature by varying the ambient relative humidity in terms of solvent-induced structural changes. The nucleic acids and phospholipids both display examples of polymorphism on the one hand and structural conservatism on the other; even closely related representatives behave differently in this respect. DNA undergoes a hydration-driven A-B conformational transition, but RNA maintains an A-like structure independently of the water activity. Similarly, a main transition between the solid and liquid-crystalline phases can be induced lyotropically in certain phosphatidylcholines, while their phosphatidylethanolamine counterparts do not exhibit chain melting under the same conditions. A principal difference concerning the structural changes that occur in the studied biomolecules is given by the relevant water-substrate stoichiometries. These are rather high in DNA and often low in phospholipids, suggesting different mechanisms of action of the hydration water that appears to induce structural changes on global- and local-mode levels, respectively.

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Section: Methodsmentioning

confidence: 99%

Hydration of biological molecules: Lipids versus nucleic acids

Pohle

Gauger

Dornberger³

et al. 2002

Biopolymers

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“…Various methods of DNA film preparation have been reported; they include: "wet-spinning" DNA fibers, in a parallel arrangement, around a spindle (35); stroking DNA gels (36) or viscous DNA solutions (13,37,38) with a spatula; oven-drying a DNA gel (39); and allowing a sample of polymer powder, rolled onto a plate, to humidify (5). Our novel approach, involving the slow evaporation of a dilute DNA solution, offers several advantages: It is much easier to implement than wet spinning; it does not require a custom-designed wet-spinning apparatus; and, unlike earlier approaches, it is useful for growing large, visibly uniform films.…”

Section: Film Preparationmentioning

confidence: 99%

The Effect of Packing and Conformation on Free Radical Yields in Films of Variably Hydrated DNA

Milano¹,

Bernhard²

1999

Radiation Research

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This work investigates the direct-type action of radiation (involving electron addition and electron abstraction) on DNA. Specifically, the effects of DNA hydration, conformation and packing on free radical yields are examined. The fact that these variables are interdependent complicates the analysis of how each variable affects free radical yields. The hydration dependence of free radical yields in films of both Li and Na DNA was examined. At low levels of DNA hydration (less than 25 waters per nucleotide), the relatively high free radical yields and the lack of water-derived radicals are evidence that damage transfer from the DNA solvation shell to the DNA molecule occurs. The scatter of measured free radical yields is significant (50-70%) in Li DNA, while in Na DNA it is much less (<25%). Our conclusions hinge upon two known differences between Li DNA and Na DNA: (1) At low DNA hydrations, the conformation of Na DNA undergoes several changes with increasing hydration, while the conformation of Li DNA is relatively constant over the same range. (2) Compared to Na DNA, Li DNA is more prone to self-associate, giving rise to macroscopic and microscopic crystalline domains in Li DNA films. The greater scatter of free radical yields in Li DNA films is therefore attributed to variability in packing. By virtue of the greater reproducibility of free radical yields in Na DNA films, the effects of DNA packing, conformation and hydration can be ascertained. In Na DNA, hydration-dependent changes in free radical yields are attributed primarily to changes in DNA packing.

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“…The wet-spinning method and equipment used to prepare highly oriented DNA films have been described elsewhere (15)(16)(17), as well as the transfer of thin oriented films on infrared transparent materials and the preparation of DNA-ligand complexes (18). The DNA complexes with L-carnosine, violamycin BI and with netropsin at rt = 0.2 were prepared by bathing the DNA film in a solution of the ligand in 80% ethanol.…”

Section: Corp (Usa)mentioning

confidence: 99%

Infrared linear dichroism of oriented DNA-ligand complexes prepared with the wet-spinning method

Fritzsche¹,

Rupprecht

Richter³

1984

Nucl Acids Res

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Oriented DNA films prepared by the wet-spinning technique have been complexed with several ligands: the anthracycline antibiotic violamycin BI, the dipeptide L-carnosine, and the oligopeptide antibiotic netropsin. The formation of the DNA-ligand complexes is accompanied by dramatic changes of the conformational flexibility of DNA. The B-A transition which occurs usually between 80% and 70% relative humidity (RH) is more or less suppressed by the ligands. Violamycin BI at a total ligand per DNA base pair ratio, r , of '0.03 and L-carnosine at rt X' 1.5 inhibit the B-A transition of "'18 and "'0.25 base pairs per ligand molecule, respectively. Netropsin at rt = 0.2 induces a very stable B-DNA even at rather low RH (23 %). The total hydration of this complex is significantly higher than for a drug-free DNA film. Netropsin-DNA complexes at r of 0.02 and 0.01 result in an inhibition of "45 base pairs pe4 drug molecule with respect to the B-A transition.

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Preparation of Oriented DNA by Wet Spinning.

Cited by 109 publications

References 0 publications

Hydration of biological molecules: Lipids versus nucleic acids

Hydration of biological molecules: Lipids versus nucleic acids

The Effect of Packing and Conformation on Free Radical Yields in Films of Variably Hydrated DNA

Infrared linear dichroism of oriented DNA-ligand complexes prepared with the wet-spinning method

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