Preparation of Papain Complexes with Chitosan Microparticles and Evaluation of Their Stability Using the Enzyme Activity Level

Olshannikova, Svetlana; Red’ko, Yu. A.; Lavlinskaya, Maria S.; Сорокин, А. В.; Holyavka, M. G.; Artyukhov, V. G.

doi:10.1007/s11094-022-02564-8

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…Noteworthy, the proteolytic activity toward azocollagen was the lowest, yet not negligible, suggesting the potential cosmeceutical use of this enzyme system. Similar proteolytic activity, using azocollagen as substrate, is also displayed by the popular plant proteases bromelain [78] and papain [79]. Higher specific activity was observed at pH 9 for all three substrates used in the assay.…”

Section: Effects Of Different Ph Metal Ions and Different Protein Sub...supporting

confidence: 54%

Development of Enzyme-Based Cosmeceuticals: Studies on the Proteolytic Activity of Arthrospira platensis and Its Efficient Incorporation in a Hydrogel Formulation

Ioannou

Labrou

2022

Cosmetics

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Microalgae are a valuable source of enzymes and active ingredients due to their biochemical variability, health-beneficial properties, lower production cost and viability on an industrial scale. In addition, the growing demand for sustainable products constitutes microalgae as a viable resource for the development of a range of innovative products. In the present work, the proteolytic activity of Arthrospira platensis lysate was assessed and characterized using kinetics analysis and zymography employing different substrates, pH values and divalent ions. The results suggest the presence of two main proteolytic enzymes in the lysate. In addition, a rapid and easy purification protocol of the proteolytic activity, under mild conditions, was developed using a polyethyleneglycol (PEG)/phosphate aqueous two-phase system (ATPS). The proteolytic activity of A. platensis lysate was used to develop a hydrogel formulation as an enzyme-based cosmeceutical, with potential application as a topical exfoliating agent. The incorporation of the A. platensis extract in the developed hydrogel formulation significantly improved its operational stability over time, which is a significant advantage in enzyme-based product development.

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Section: Effects Of Different Ph Metal Ions and Different Protein Sub...supporting

confidence: 54%

Development of Enzyme-Based Cosmeceuticals: Studies on the Proteolytic Activity of Arthrospira platensis and Its Efficient Incorporation in a Hydrogel Formulation

Ioannou

Labrou

2022

Cosmetics

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“…Our previously studies show that natural polymers, such as chitosan [2,14] and its derivatives [21][22][23][24] or nanoparticles based on it [25,26] are promising carriers for cysteine protease immobilization. This process can lead to enhance of proteolytic activity and make it possible to use immobilized enzymes as antibacterial and antibiofilm agents as well as in wound healing [2,27].…”

Section: Introductionmentioning

confidence: 99%

Complexation of Bromelain, Ficin, and Papain with the Graft Copolymer of Carboxymethyl Cellulose Sodium Salt and N-Vinylimidazole Enhances Enzyme Proteolytic Activity

Сорокин

Goncharova

Lavlinskaya

et al. 2023

IJMS

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This study investigates the features of interactions between cysteine proteases (bromelain, ficin, and papain) and a graft copolymer of carboxymethyl cellulose sodium salt with N-vinylimidazole. The objective is to understand the influence of this interactions on the proteolytic activity and stability of the enzymes. The enzymes were immobilized through complexation with the carrier. The interaction mechanism was examined using Fourier-transform infrared spectroscopy and flexible molecular docking simulations. The findings reveal that the enzymes interact with the functional groups of the carrier via amino acid residues, resulting in the formation of secondary structure elements and enzyme’s active sites. These interactions induce modulation of active site of the enzymes, leading to an enhancement in their proteolytic activity. Furthermore, the immobilized enzymes demonstrate superior stability compared to their native counterparts. Notably, during a 21-day incubation period, no protein release from the conjugates was observed. These results suggest that the complexation of the enzymes with the graft copolymer has the potential to improve their performance as biocatalysts, with applications in various fields such as biomedicine, pharmaceutics, and biotechnology.

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“…It is known that the dissolved forms of enzymes are characterized by low stability, which limits their use in drug development. This problem can be solved by immobilizing the enzyme on an inert or antibacterial matrix [ 13 , 14 , 15 , 16 , 17 , 18 ]. Immobilization increases the rigidity of the tertiary structure of the enzyme molecules, thereby stabilizing it and increasing the half-life [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Immobilized Biocatalysts Based on Cysteine Proteases Bound to 2-(4-Acetamido-2-sulfanilamide) Chitosan and Research on Their Structural Features

et al. 2022

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Briefly, 2-(4-Acetamido-2-sulfanilamide) chitosan, which is a chitosan water-soluble derivative, with molecular weights of 200, 350, and 600 kDa, was successfully synthesized. The immobilization of ficin, papain, and bromelain was carried out by complexation with these polymers. The interaction mechanism of 2-(4-acetamido-2-sulfanilamide) chitosan with bromelain, ficin, and papain was studied using FTIR spectroscopy. It was found that the hydroxy, thionyl, and amino groups of 2-(4-acetamido-2-sulfanilamide) chitosan were involved in the complexation process. Molecular docking research showed that all amino acid residues of the active site of papain formed hydrogen bonds with the immobilization matrix, while only two catalytically valuable amino acid residues took part in the H-bond formation for bromelain and ficin. The spectral and in silico data were in good agreement with the catalytic activity evaluation data. Immobilized papain was more active compared to the other immobilized proteases. Moreover, the total and specific proteolytic activity of papain immobilized on the carrier with a molecular weight of 350 kDa were higher compared to the native one due to the hyperactivation. The optimal ratio of protein content (mg × g −1 of carrier), total activity (U × mL−1 of solution), and specific activity (U × mg−1 of protein) was determined for the enzymes immobilized on 2-(4-acetamido-2-sulfanilamide) chitosan with a molecular weight of 350 kDa.

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Preparation of Papain Complexes with Chitosan Microparticles and Evaluation of Their Stability Using the Enzyme Activity Level

Cited by 5 publications

References 18 publications

Development of Enzyme-Based Cosmeceuticals: Studies on the Proteolytic Activity of Arthrospira platensis and Its Efficient Incorporation in a Hydrogel Formulation

Development of Enzyme-Based Cosmeceuticals: Studies on the Proteolytic Activity of Arthrospira platensis and Its Efficient Incorporation in a Hydrogel Formulation

Complexation of Bromelain, Ficin, and Papain with the Graft Copolymer of Carboxymethyl Cellulose Sodium Salt and N-Vinylimidazole Enhances Enzyme Proteolytic Activity

Novel Immobilized Biocatalysts Based on Cysteine Proteases Bound to 2-(4-Acetamido-2-sulfanilamide) Chitosan and Research on Their Structural Features

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