Preparation of pentacyclic triterpenoid-rich Centella asiatica extract and its pharmacological activities

Preparation, development, and scale-up of standardized pentacyclic triterpenoid-rich extract from Centella asiatica (L.) Urb. and study of its wound healing activity

“…Moreover, the CAE-L-2 C –B column also helped separate the aglycones and glycosides. In addition, the amount of pentacyclic triterpenes obtained was equivalent to the previous extraction method [ 15 , 16 , 35 ], but this approach reduced the number of steps of the initial extract preparation and TLC determination.…”

“…However, the active constituent of C. asiatica in the raw material varies due to different factors such as extraction method, solvent for extraction, etc. [ [14] , [15] , [16] ]. Therefore, developing a fast and efficient way to prepare a large quantity of high-quality standardized extract with easy-to-control levels of each component will benefit the pharmaceutical, cosmeceutical, and food industries.…”

“…In a standardized extract, it is easy to reproducibly control the level of each component. Moreover, recent trends in extraction techniques have focused on green extraction methods that can reduce the volume of solvents and energy needed, reduce the number of production steps, use renewable natural materials that are safe, increase the yield of the extract and product quality, and are easily transferred from laboratory experiments to the industrial scale [ 15 , 16 , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] ]. At present, the industrial scale uses several methods for preparing the crude extract, such as microwave-assisted extraction (MAE) [ 27 ], pressurized hot water flow-through extraction system [ 28 ], supercritical carbon dioxide (SC–CO 2 ) extraction [ 29 ], ohmic heating-assisted extraction (OHAE) [ 30 ], and ultrasonic-assisted extraction (UAE) [ 31 ].…”

“…This study developed a new method to prepare PRE modified from the studies of Puttarak and co-workers [ 15 , 16 ]. This previous study used MAE to prepare the C. asiatica extract.…”

FORMULATION OF FORTIFIED MEDIA CONSISTING OF OIL PALM EMPTY FRUIT BUNCH BIOCOMPOST AND OIL PALM KERNEL SHELL BIOCHAR FOR THE ENHANCEMENT OF BIOACTIVE COMPOUNDS IN Centella asiatica (L.) URBAN

“…Studies conducted by Alqahtani et al (2015) have suggested that C. asiatica, which originates in Australia, exhibits higher amounts of triterpenoids during the summer season, indicating an optimum time for harvesting. Similarly, research by Puttarak and Panichayupakaranant (2012) has documented that different provenance in Thailand and cultivation during different months also greatly influence the yield of bioactive compounds in C. asiatica. This highlights the potential impact of geographical location and harvesting time on the triterpenoid content of C. asiatica.…”