Preparation of Phillygenin-Hyaluronic acid composite milk-derived exosomes and its anti-hepatic fibrosis effect

Gong, Lihong; Zhou, Honglin; Zhang, Yafang; Wang, Cheng; Fu, Ke; Ma, Cheng; Li, Yunxia

doi:10.1016/j.mtbio.2023.100804

Cited by 6 publications

(1 citation statement)

References 44 publications

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“…Several researchers synthetically incorporated glycolipids presenting CD47 "don’t eat me" protein and an αvβ3-integrin targeting peptide, and these glycolipid-exosomes evaded phagocytosis and homed to angiogenic blood vessels [ 52 ]. More recently, Gong et al generated exosomes displaying hyaluronic acid by expressing a membrane anchor protein fused with a hyaluronic acid-binding domain [ 53 ], and they demonstrated that the hyaluronic acid-displaying exosomes showed enhanced anti-hepatic fibrosis effect by interacting with CD44 receptors on tumor cells.…”

Section: Decoration Of Exosome For Drug Delivery and Targeted Therapymentioning

confidence: 99%

Encapsulation and assessment of therapeutic cargo in engineered exosomes: a systematic review

Chen,

Xiong,

Tian

et al. 2024

J Nanobiotechnol

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Exosomes are nanoscale extracellular vesicles secreted by cells and enclosed by a lipid bilayer membrane containing various biologically active cargoes such as proteins, lipids, and nucleic acids. Engineered exosomes generated through genetic modification of parent cells show promise as drug delivery vehicles, and they have been demonstrated to have great therapeutic potential for treating cancer, cardiovascular, neurological, and immune diseases, but systematic knowledge is lacking regarding optimization of drug loading and assessment of delivery efficacy. This review summarizes current approaches for engineering exosomes and evaluating their drug delivery effects, and current techniques for assessing exosome drug loading and release kinetics, cell targeting, biodistribution, pharmacokinetics, and therapeutic outcomes are critically examined. Additionally, this review synthesizes the latest applications of exosome engineering and drug delivery in clinical translation. The knowledge compiled in this review provides a framework for the rational design and rigorous assessment of exosomes as therapeutics. Continued advancement of robust characterization methods and reporting standards will accelerate the development of exosome engineering technologies and pave the way for clinical studies. Graphical Abstract

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Section: Decoration Of Exosome For Drug Delivery and Targeted Therapymentioning

confidence: 99%

Encapsulation and assessment of therapeutic cargo in engineered exosomes: a systematic review

Chen,

Xiong,

Tian

et al. 2024

J Nanobiotechnol

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Chemo‐Enzymatic Functionalization of Bovine Milk Exosomes with an EGFR Nanobody for Target‐specific Drug Delivery

Zhang,

Li,

Zhou

et al. 2024

ChemBioChem

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Bovine milk exosomes (BmExo) have been identified as versatile nanovesicles for anti‐cancer drugs delivery due to their natural availability and biocompatibility. However, tumor‐specific delivery based on BmExo often requires post‐isolation modifications of the membrane surface with active‐targeting ligands. In this study, we report an alternative approach to functionalize BmExo with nanobody using Sortase A‐mediated site‐specific ligation for drug delivery. The BmExo membrane was first coated with a diglycine‐containing amphiphile molecule, NH2‐GG‐PEG2000‐DSPE, through hydrophobic insertion, following by ligation with EGFR nanobody (7D12) by Sortase A (SrtA). The successful construction of BmExo‐7D12 was confirmed by Western blotting analysis, electron microscopy, and dynamic light scattering (DLS). As a demonstration model, BmExo‐7D12 loaded with the chemotherapeutic drug doxorubicin (Dox) was shown to be able to deliver Dox to cancer cells in response to the expression of EGFR as manifested by immunocytochemistry and flow cytometry analysis. Finally, the cytotoxicity assay showed that BmExo‐7D12‐Dox was more effective in killing tumor cells with high EGFR expression while significantly reduced the non‐specific toxicity to EGFR negative cells. This study developed an effective approach to functionalize BmExo with nanobody for target‐specific drug delivery. This approach should prove to be versatile and efficient for the generation of protein‐ligands modified BmExo.

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Milk‐derived exosomes in the regulation of nutritional and immune functions

Yang,

Wuren,

Zhai

et al. 2024

Food Science & Nutrition

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Milk‐derived exosomes (MDEs), being a component of milk, have the potential to support immune system maturation in offspring and enhance immune cell proliferation. Through the transport and transmission of essential signaling molecules, MDEs contribute to the regulation of intergenerational and intraspecies communication, thereby impacting nutrient uptake and metabolic functions. A comprehensive comprehension of MDE functionalities is imperative for enhancing the quality of the dairy industry. A systematic search of the databases PubMed/Medline, Web of Science, and Scopus utilizing predetermined keywords resulted in the identification of 418 articles, of which 67 were chosen for inclusion in this review, which specifically explores the intersection of immune response and nutrition. This article provides a critical analysis of the classification of extracellular vesicles, the mechanisms underlying the biosynthesis of microvesicular dietary exosomes (MDEs), the components of MDEs, and their relevance in the contexts of nutrition and immune modulation. The primary aim of this review was to offer valuable scholarly insights to support the advancement and practical application of MDEs.

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Preparation of Phillygenin-Hyaluronic acid composite milk-derived exosomes and its anti-hepatic fibrosis effect

Cited by 6 publications

References 44 publications

Encapsulation and assessment of therapeutic cargo in engineered exosomes: a systematic review

Encapsulation and assessment of therapeutic cargo in engineered exosomes: a systematic review

Chemo‐Enzymatic Functionalization of Bovine Milk Exosomes with an EGFR Nanobody for Target‐specific Drug Delivery

Milk‐derived exosomes in the regulation of nutritional and immune functions

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