Preparation of PLLA membranes with different morphologies for culture of MG-63 Cells

Liu, Hwa‐Chang; Lee, I‐Chi; Wang, Jyh-Horng; Yang, Shuai; Young, Tai‐Horng

doi:10.1016/j.biomaterials.2003.10.098

Cited by 72 publications

(57 citation statements)

References 26 publications

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“…The culture of cells on a biodegradable polymer substrate may cause changes in cell morphology and/or function. 17,23 Given the fact that the cells were grown on the same polymer material, we believe that the adoption of PLLA for fabricating patterns will not compromise our conclusion on the comparison of projection areas and VGCC responsiveness for cells of the 2D, 3D, and N2D categories. Moreover, the present study of cellular functionality (VGCC responsiveness) in relation to cell morphology or spreading for cells on a biodegradable polymer substrate offers a useful approach for addressing cell-material interactions in research fields of biomaterials and tissue engineering.…”

Section: L-type Calcium Channel Stainingmentioning

confidence: 99%

“…PLLA is a popular biodegradable polymer with excellent biocompatibility [16][17][18] and, when properly structured, it supports various forms of scaffolds including membrane, 23 nanofiber, 24 or sponge 25,26 for tissue engineering purposes. However, the establishment of 3D cell-based assays with the same scaleddown materials is not automatic for the following reasons.…”

Section: Fabrication Of the Plla Microwell Patternsmentioning

confidence: 99%

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Responsiveness of voltage-gated calcium channels in SH-SY5Y human neuroblastoma cells on quasi-three-dimensional micropatterns formed with poly (l-lactic acid)

Wang²,

Zhang³

et al. 2013

IJN

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Introduction:In this study, quasi-three-dimensional (3D) microwell patterns were fabricated with poly (l-lactic acid) for the development of cell-based assays, targeting voltage-gated calcium channels (VGCCs). Methods and materials: SH-SY5Y human neuroblastoma cells were interfaced with the microwell patterns and found to grow as two dimensional (2D), 3D, and near two dimensional (N2D), categorized on the basis of the cells' location in the pattern. The capability of the microwell patterns to support 3D cell growth was evaluated in terms of the percentage of the cells in each growth category. Cell spreading was analyzed in terms of projection areas under light microscopy. SH-SY5Y cells' VGCC responsiveness was evaluated with confocal microscopy and a calcium fluorescent indicator, Calcium Green TM -1. The expression of L-type calcium channels was evaluated using immunofluorescence staining with DM-BODIPY. Results: It was found that cells within the microwells, either N2D or 3D, showed more rounded shapes and less projection areas than 2D cells on flat poly (l-lactic acid) substrates. Also, cells in microwells showed a significantly lower VGCC responsiveness than cells on flat substrates, in terms of both response magnitudes and percentages of responsive cells, upon depolarization with 50 mM K + . This lower VGCC responsiveness could not be explained by the difference in L-type calcium channel expression. For the two patterns addressed in this study, N2D cells consistently exhibited an intermediate value of either projection areas or VGCC responsiveness between those for 2D and 3D cells, suggesting a correlative relation between cell morphology and VGCC responsiveness. Conclusion: These results suggest that the pattern structure and therefore the cell growth characteristics were critical factors in determining cell VGCC responsiveness and thus provide an approach for engineering cell functionality in cell-based assay systems and tissue engineering scaffolds.

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Section: L-type Calcium Channel Stainingmentioning

confidence: 99%

Section: Fabrication Of the Plla Microwell Patternsmentioning

confidence: 99%

Responsiveness of voltage-gated calcium channels in SH-SY5Y human neuroblastoma cells on quasi-three-dimensional micropatterns formed with poly (l-lactic acid)

Wang²,

Zhang³

et al. 2013

IJN

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“…22 After overnight attachment, 500 μ L of the material extracts were added into each well. Negative control was composed of 500 μ L of DMEM supplemented with 2% fetal bovine serum and 1% antibiotics.…”

Section: Xtt Assaymentioning

confidence: 99%

Biocompatibility of experimental mixture of mineral trioxide aggregate and glass ionomer cement

Jeong

Bae

et al. 2010

J Korean Acad Conserv Dent

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Objectives:The purpose of the present in vitro study was to evaluate the biocompatibility of mineral trioxide aggregate (MTA) mixed with glass ionomer cement (GIC), and to compare it with that of MTA, GIC, IRM and SuperEBA. Materials and Methods: Experimental groups were divided into 3 groups such as 1 : 1, 2 : 1, and 1 : 2 groups depending on the mixing ratios of MTA powder and GIC powder. Instead of distilled water, GIC liquid was mixed with the powder. This study was carried out using MG-63 cells derived from human osteosarcoma. They were incubated for 1 day on the surfaces of disc samples and examined by scanning electron microscopy. To evaluate the cytotoxicity of test materials quantitatively, XTT assay was used. The cells were exposed to the extracts and incubated. Cell viability was recorded by measuring the optical density of each test well in reference to controls. Results: The SEM revealed that elongated, dense, and almost confluent cells were observed in the cultures of MTA mixed with GIC, MTA and GIC. On the contrary, cells on the surface of IRM or SuperEBA were round in shape. In XTT assay, cell viability of MTA mixed with GIC group was similar to that of MTA or GIC at all time points. IRM and SuperEBA showed significantly lower cell viability than other groups at all time points (p < 0.05).

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“…Meanwhile, the proliferation rates of MC3T3-E1 cells treated with DCP5-rGO2.5 and DCP5-rGO5 composites were decreased at 14 days. In general, the proliferation of various types of cells is retarded when the cells begin to differentiate into specific cell lineages under in vitro conditions [71][72][73]. We therefore examined the osteogenic differentiation of MC3T3-E1 cells to further investigate the effects of DCP-rGO composites.…”

Section: Methodsmentioning

confidence: 99%

Dicalcium Phosphate Coated with Graphene Synergistically Increases Osteogenic Differentiation In Vitro

et al. 2017

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Abstract:In recent years, graphene and its derivatives have attracted much interest in various fields, including biomedical applications. In particular, increasing attention has been paid to the effects of reduced graphene oxide (rGO) on cellular behaviors. On the other hand, dicalcium phosphate (DCP) has been widely used in dental and pharmaceutical fields. In this study, DCP composites coated with rGO (DCP-rGO composites) were prepared at various concentration ratios (DCP to rGO concentration ratios of 5:2.5, 5:5, and 5:10 µg/mL, respectively), and their physicochemical properties were characterized. In addition, the effects of DCP-rGO hybrid composites on MC3T3-E1 preosteoblasts were investigated. It was found that the DCP-rGO composites had an irregular granule-like structure with a diameter in the range order of the micrometer, and were found to be partially covered and interconnected with a network of rGO. The zeta potential analysis showed that although both DCP microparticles and rGO sheets had negative surface charge, the DCP-rGO composites could be successfully formed by the unique structural properties of rGO. In addition, it was demonstrated that the DCP-rGO composites significantly increased alkaline phosphatase activity and extracellular calcium deposition, indicating that the DCP-rGO hybrid composites can accelerate the osteogenic differentiation by the synergistic effects of rGO and DCP. Therefore, in conclusion, it is suggested that the DCP-rGO hybrid composites can be potent factors in accelerating the bone tissue regeneration.

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Preparation of PLLA membranes with different morphologies for culture of MG-63 Cells

Cited by 72 publications

References 26 publications

Responsiveness of voltage-gated calcium channels in SH-SY5Y human neuroblastoma cells on quasi-three-dimensional micropatterns formed with poly (l-lactic acid)

Responsiveness of voltage-gated calcium channels in SH-SY5Y human neuroblastoma cells on quasi-three-dimensional micropatterns formed with poly (l-lactic acid)

Biocompatibility of experimental mixture of mineral trioxide aggregate and glass ionomer cement

Dicalcium Phosphate Coated with Graphene Synergistically Increases Osteogenic Differentiation In Vitro

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