Preparation of Poly(glycidyl methacrylate) (PGMA) and Amine Modified PGMA Adsorbents for Purification of Glucosinolates from Cruciferous Plants

Abstract

Glucosinolates (GLs) are of great interest for their potential as antioxidant and anticancer compounds. In this study, macroporous crosslinked copolymer adsorbents of poly (glycidyl methacrylate) (PGMA) and its amine (ethylenediamine, diethylamine, triethylamine)-modified derivatives were prepared and used to purify the GLS glucoerucin in a crude extract obtained from a cruciferous plant. These four adsorbents were evaluated by comparing their adsorption/desorption and decolorization performance for the purification of glucoerucin from crude plant extracts. According to the results, the strongly basic triethylamine modified PGMA (PGMA-III) adsorbent showed the best adsorption and desorption capacity of glucoerucin, and its adsorption data was a good fit to the Freundlich isotherm model and pseudo-second-order kinetics; the PGMA adsorbent gave the optimum decolorization performance. Furthermore, dynamic adsorption/desorption experiments were carried out to optimize the purification process. Two glass columns were serially connected and respectively wet-packed with PGMA and PGMA-III adsorbents so that glucoerucin could be decolorized and isolated from crude extracts in one process. Compared with KCl solution, aqueous ammonia was a preferable desorption solvent for the purification of glucoerucin and overcame the challenges of desalination efficiency, residual methanol and high operation costs. The results showed that after desorption with 10% aqueous ammonia, the purity of isolated glucoerucin was 74.39% with a recovery of 80.63%; after decolorization with PGMA adsorbent, the appearance of glucoerucin was improved and the purity increased by 11.30%. The process of using serially connected glass columns, wet-packed with PGMA and PGMA-III, may provide a simple, low-cost, and efficient method for the purification of GLs from cruciferous plants.

Air-permeable and flexible multifunctional cellulose-based textiles for bio-protection, thermal heating conversion, and electromagnetic interference shielding

“…8 CNs-P@M-PG showed absorption bands at 1250, 908, and 840 cm À1 (epoxide ring deformation) and 1724 cm À1 (O] C-O tensile deformation), indicating the successful polymerization of PGMA. 52,55,56 At the same time, the new absorption bands of CNs-P@M-PG-NEO and CNs-P@M-PG-PHGH at 1633 cm À1 (C]N stretching vibration) and 1309 cm À1 (C-N stretching) conrmed the existence of NEO and PHGH. 41,52,57 According to the wide scan spectra of X-ray photoelectron spectroscopy (XPS) (Fig.…”

Preparation of a Series of Highly Efficient Porous Adsorbent PGMA-N Molecules and Its Application in the Co-Removal of Cu(II) and Sulfamethoxazole from Water

“…The preparation of highly efficient adsorbents has significant value and promising prospects for solving water pollution problems. Poly(glycidyl methacrylate) (PGMA) is a commonly used adsorbent material with an epoxy functional group, which facilitates various modifications [36]. PGMA was used as the substrate because of its known good mechanical strength and high reactivity of the epoxy groups for surface grafting.…”

Preparation of a series of highly efficient porous adsorbent PGMA- N and its application in the co-removal of Cu(II) and sulfamethoxazole from water

“…[1][2][3][4] . Antibiotics mainly originate from human and animal use and medical and industrial wastewater discharge [5][6][7] . The widespread use and discharge of antibiotics can increase their concentration in natural water bodies, affecting aquatic organisms and aquatic ecosystems [8,9] .…”