Preparation of poly(N-isopropylacrylamide) copolymers and preliminary assessment of their acute and subacute toxicity in mice

Malonne, Hugues; Eeckman, Frédéric; Fontaine, David; Otto, Anne; Vos, Louis De; Moes, André Jules; Fontaine, Jeanine; Amighi, Karim

doi:10.1016/j.ejpb.2005.05.007

Cited by 95 publications

(61 citation statements)

References 17 publications

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“…In this respect, PNIPAAm has been shown to exhibit no cytotoxicity under cell culture conditions (Takezawa et al, 1990) and no detectable oral toxicity in rodents (Malonne et al, 2005). Furthermore, PNIPAAm was used in intelligent drug delivery system studies (Fang et al, 2008;Quan et al, 2008) and as a scaffold for tissue engineering (Ibusuki et al, 2003;Newman et al, 2006), which also facilitates the use of this polymer in animals.…”

Section: Discussionmentioning

confidence: 99%

Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability

Fujita¹,

Shimizu²,

Nagamori³

2009

Biotech & Bioengineering

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We have succeeded in fabricating a cell sheet-polymer film complex involving a temperature-sensitive polymer that has enough mechanical strength that can be manipulated even by forceps. The polymer film can be removed by lowering the temperature after transplantation, demonstrating its potential use in regenerative medicine. Recently, tissue engineering involving cell sheets was developed, tissues being fabricated by layering of these cell sheets. This technique promises high density cell packing, which is important for native cell functions, and successful heart therapy using cardiac cell sheets has been reported. On the other hand, the fabrication of a large tissue using cell sheets is difficult because of fragility of the cell sheets. Here, we have developed a novel method in which cells are attached to a temperature-sensitive poly-N-isopropylacrylamide film mixed with laminin and collagen IV, and report that the cell sheet-polymer film complex can be manipulated with forceps. A cell sheet can be removed from the polymer film by lowering the temperature after the manipulation. We have utilized this technique for the primary myocardium and fabricated a physiologically active multi-layered cardiac cell sheet. By applying a micropattern to this polymer film, we have succeeded in making a skeletal muscle cell sheet in which myotubes are oriented in the desired direction. Overall, we showed that this method is useful for cell sheet manipulation, morphogenesis, and transplantation.

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Section: Discussionmentioning

confidence: 99%

Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability

Fujita¹,

Shimizu²,

Nagamori³

2009

Biotech & Bioengineering

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“…PNIPAAM has a lower critical solution temperature (LCST) around 32°C [29]. However, the cloud point of PNIPAAM changes with concentration [23] and when it is copolymerized with other monomers [30][31][32][33][34]. Copolymerizing of NIPAAM with a hydrophilic monomer, such as acrylic acid, will shift the LCST to a higher temperature [34].…”

Section: Introductionmentioning

confidence: 99%

Characterization of temperature induced changes in liposomes coated with poly( N -isopropylacrylamide- co -methacrylic acid)

Klemetsrud

Hiorth

Smistad

et al. 2015

Journal of Colloid and Interface Science

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“…The convenient LCST, biocompatibility, and relative nontoxicity up to a certain concentration [13] give advantage to PNIPAAm in comparison with other thermosensitive polymers, particularly in the field of controlled drug delivery systems, biomedical engineering, and pharmaceutical formulations. PNIPAAm-based materials could be found in a myriad of fields ranging from microelectronics, medicine, pharmacy, microbiology, agriculture, bioengineering, and development of thermosensitive textiles [5,6,14].…”

Section: Poly(n-isopropylacrylamide): Pnipaam Basic Facts and Propertiesmentioning

confidence: 99%

Novel Synthesis Pathways for PNIPAAm-Based Hydrogels and Their Application in Thermosensitive Textiles

Jovančić

Petruš̆̌̌̌ić

Molina

2014

Handbook of Smart Textiles

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The research focus is lately directed towards the development of the thermosensitive textiles with advanced functionalities. As sources of inspiration for a progress in the field of these innovative materials, stimuli-sensitive polymer engineering, plasma surface engineering, and nanotechnology have been extensively explored. In this work, the polymerization methods for synthesis of Poly(N-isopropylacrylamide), PNIPAAm-based hydrogels, incorporating natural biopolymers (chitosan and b-cyclodextrin), for achieving dual temperature/pH sensitivity and improvement of relevant loading capacity towards specific guest molecules are outlined. These PNIPAAm-based hydrogels are of great interest for biomedical applications (transdermal drug delivery, cell encapsulation, wound dressing, tissue repair, etc.) and intelligent stimuli-sensitive textile coatings for moisture regain control, antimicrobial properties, water and/or oil repellency, and fragrance release. The strategies for synthesis of PNIPAAm-based hydrogels (macro and micro forms) and their proper integration onto textiles through the plasma surface modification, graft polymerization, and coatings are discussed. Novel possibilities for creating hydrogels based on atmospheric plasma polymerization of NIPAAm monomers in liquids have alos been reported. Plasma polymerization in liquids demonstrated a great technological potential given that it may be performed without the use of chemical initiators and even without cross-linking agents, offering multiple advantages over the conventional hydrothermal polymerization methods. Further research on adequate chemical and physical structures of PNIPAAm-based hydrogels and methods of their applications in textiles could boost a progress in the domain of smart textiles, implying novel performances and end uses.

show abstract

Preparation of poly(N-isopropylacrylamide) copolymers and preliminary assessment of their acute and subacute toxicity in mice

Cited by 95 publications

References 17 publications

Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability

Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability

Characterization of temperature induced changes in liposomes coated with poly( N -isopropylacrylamide- co -methacrylic acid)

Novel Synthesis Pathways for PNIPAAm-Based Hydrogels and Their Application in Thermosensitive Textiles

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