Preparation of polymer monolithic column functionalized by arsonic acid groups for mixed-mode capillary liquid chromatography

Qin, Zhang-Na; Yu, Quan; Wang, Ren-Qi; Feng, Yu‐Qi

doi:10.1016/j.chroma.2018.03.007

Cited by 17 publications

(4 citation statements)

References 34 publications

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“…For fabricating ZrAs‐IMAC monolithic column, fused‐silica capillaries (530 μm i.d.) were purchased from Yongnian Optic Fiber Plant (Hebei, China), 3‐(triethoxysilyl)propyl methacrylate was obtained from Wuhan University Silicone New Material (Wuhan, China), p ‐MAPHA was synthesized and characterized by MS and 1 H NMR according to our previous study [44], EDMA was supplied by Acros (New Jersey, USA), azobisisobutyronitrile (AIBN), PEG 20000, zirconyl chloride octahydrate (ZrOCl 2 ·8H 2 O), and methanol were obtained from Shanghai Chemical Reagent Corp. (Shanghai, China). For phosphopeptides analysis, HPLC‐grade ACN was purchased from Fisher Scientific (Pittsburgh, PA, USA), TFA, phosphoric acid (H 3 PO 4 ), 2, 5‐dihydroxybenzoic acid (2,5‐DHB), bovine β‐casein (containing a small amount of α‐casein), and BSA were supplied by Sigma–Aldrich (St. Louis, MO, USA), aqueous ammonia solution (25 wt%) was purchased from Sinopharm Chemical Reagent (Shanghai, China), TPCK‐treated trypsin was obtained from Worthington Biochemical (New Jersey, USA), and biological samples including human serum was supplied by the hospital of Wuhan University according to the standard clinical procedures, and sprague dawley male rats (ca.…”

Section: Methodsmentioning

confidence: 99%

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Preparation of zirconium arsenate‐modified monolithic column for selective enrichment of phosphopeptides

Qin

Chen

et al. 2020

J of Separation Science

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Protein phosphorylation is a crucial posttranslational modification for the regulation of many different biological functions. Selective enrichment of phosphopeptides from the complex biological samples is an essential step for the mass spectrometry analysis of protein phosphorylation. In this study, an arsenate functionalized monolithic column was first prepared by a single‐step copolymerization of p‐methacryloylaminophenylarsonic acid and ethylene dimethacrylate. Then the metal ions Zr4+ were attached onto the prepared monolithic column via metal‐chelate complex formation by Zr4+ and arsenate groups. The obtained monolithic column was employed as a new sorbent for the phosphopeptide enrichment via immobilized metal affinity chromatography. Phosphopeptides analysis was realized by polymer monolith microextraction using this monolithic column coupled to both matrix‐assisted laser desorption/ionization mass spectrometry and liquid chromatography–electrospray ionization tandem mass spectrometry. The proposed method exhibited a high selectivity for phosphopeptide enrichment in complex matrices, and was applied to the analysis of phosphopeptides in human serum and tryptic digests of rat brain proteins. Four phosphopeptides could be selectively captured from human serum and 2608 endogenous phosphopeptides were identified from the tryptic digests of rat brain proteins, indicating a satisfactory performance of this method for the enrichment of phosphopeptides from complex biological samples.

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Section: Methodsmentioning

confidence: 99%

“…In our previous work, an arsenate functionalized polymer monolithic column was successfully prepared by a single-step copolymerization method [44]. We herein intended to introduce an arsenate functionalized polymer monolithic column as the substrate material of IMAC-monolithic column.…”

Section: Introductionmentioning

confidence: 99%

Preparation of zirconium arsenate‐modified monolithic column for selective enrichment of phosphopeptides

Qin

Chen

et al. 2020

J of Separation Science

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“…Biopolymers interact with the monolith surface in a strong manner via multiple binding sites. Very recently, Qin et al reported the preparation of poly(p-methacryloylaminophenylarsonic acid(p-MAPHA)-co-PETA) monolithic column for mixedmode capillary LC in which p-MAPHA as a co-monomer was used [129]. The PMC thus obtained exhibited mixed mode character with cation exchange originated from p-MAPHA.…”

Section: Ion Exchange Pmcsmentioning

confidence: 99%

Organic polymer‐based monolithic capillary columns and their applications in food analysisψ

et al. 2019

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In recent years, the use of organic polymer monolithic capillary columns in separation science has gained popularity due to the fact that they are easy to fabricate and do not require retaining frits. These materials have been applied in different fields including foods, proteomics, and pharmaceuticals. The interest in food analysis still needs to develop in order to increase the sensitivity towards micro/nano‐scale food applications for food samples of < 5 μg (e.g., foodomics). In this regard, polymer monolithic capillary columns offer great separation capability in the food analytical separation science. We review the most important applications in food analysis using polymer monolithic capillary columns. In addition, several examples of the use of capillary separation methods combined with mass spectrometry detection in food analysis are summarized.

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“…A mixed‐mode monolithic column functionalized by arsonic acid groups was prepared by single‐step in situ copolymerization of monomers p ‐methacryloylaminophenylarsonic acid and pentaerythritol triacrylate . As a diprotic anionic acid has significantly different acid dissociation constants (p K a 1 ≈ 4.0 and p K a 2 ≈ 9.0) with respect to sulfonic, phosphonic, carboxylic, and boronic acid groups usually employed for generating mixed‐mode functionalities it is evident that it provides a specific and alternative selectivity of the generated stationary phase.…”

Section: Monolithic Mixed‐mode Stationary Phasesmentioning

confidence: 99%

Recent advances in mixed‐mode chromatographic stationary phases

Sýkora

Řezanka

Záruba

et al. 2018

J of Separation Science

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Mixed‐mode phases have become very popular in the last decade, and the number of new mixed/multi‐mode sorbents is growing fast. Unlike single‐mode stationary phases, perfectly suited for the separation of the analytes possessing similar physicochemical properties, for instance reversed‐phase chromatography for hydrophobic solutes, mixed‐mode sorbents providing multimodal interactions can render better separation selectivity for complex mixtures of solutes differing significantly in their physicochemical characteristics. The most frequent modern mixed‐mode stationary phases are di/tri‐mode sorbents embracing the following interactions, hydrophobic, electrostatic (coulombic), and hydrophilic. According to their structures, it is possible to distinguish silica‐based, polymer‐based, hybrid, and monolithic mixed‐mode stationary phases. Herewith, newly synthesized mixed‐mode sorbents developed within the last two and half years are categorized, discussed, and summarized. The main attention is devoted to the description of the synthetic routes and characterization methods applied for the new stationary phases.

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Preparation of polymer monolithic column functionalized by arsonic acid groups for mixed-mode capillary liquid chromatography

Cited by 17 publications

References 34 publications

Preparation of zirconium arsenate‐modified monolithic column for selective enrichment of phosphopeptides

Preparation of zirconium arsenate‐modified monolithic column for selective enrichment of phosphopeptides

Organic polymer‐based monolithic capillary columns and their applications in food analysisψ

Recent advances in mixed‐mode chromatographic stationary phases

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