Preparation of porous polymeric structures for enzyme immobilization

Abstract

Porous polymethacrylic acid-co-triethylene glycol dimethacrylate (MAA-co-3G) and polyacrylic acid-co-triethylene glycol dimethacrylate (AA-co-3G) were prepared by four different polymerization techniques, namely, suspension, dispersion, seed, and microemulsion polymerization using an inert diluent (n-hexane and polystyrene). The morphology and porosity of the obtained polymers were examined by means of a scanning electron microscope. The surface areas of the obtained polymers were determined colorimetrically. The copolymers were modified by hydroximation and chlorination using hydroxyl amine and thionyl chloride, respectively. The effect of polymerization type, surface area, modification, and pH on the protease enzyme immobilization over poly(MAA-co-3G) and poly(AA-co-3G) was examined. The enzyme activity was measured by means of a spectrophotometer. The reactivity ratios of the two monomers MAA and 3G were determined by means of the elemental analysis method.

Preparation and characterization of crosslinked polymer beads with tunable pore morphology

“…From bead W01 to W04, the values dramatically declined with decreasing DVB/St volume ratio. This result was agreement with those obtained by Moustafa et al16 and Galia et al10 Because the specific surface area was mainly originated from the surface of the nuclei (Figure 2), which were the smallest composition units of crosslinked polymer beads. Thus, the factors decreasing the size of the nuclei or increasing their stiffness could increase the specific surface area.…”

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

“…In a report on the preparation of porous poly (methacrylic acid) particles via this route [65], 11 wt% of methacrylic acid was polymerized in a chloroform/EtOH mixture (∼5/1). The obtained porous particles were then crosslinked [65][66]. The monomer concentration is much higher in the medium compared to the precipitation polymerization procedures because of the high amount (6.5 wt%) of polymeric stabilizer used.…”

Swelling behavior of gelatin‐ionic liquid functional polymers

“…. Because of their unique properties, hydrogels have been studied for many applications, such as the aforementioned enzyme immobilization,4, 5 deployment of agrochemicals and fertilizers,6, 7 and biomedical uses such as drug delivery,8 cell delivery and tissue engineering 9…”