Preparation of protein nanoparticles using AOT reverse micelles

Naoe, Kazumitsu; Yoshimoto, Shinya; Naito, Nobuhiro; Kawagoe, Mikio; Imai, Masanao

doi:10.1016/j.bej.2011.02.015

Cited by 17 publications

(10 citation statements)

References 21 publications

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“…Dynamics equation is based on the two-film theory, thus the suitability of using this model to reverse micelle system can be obtained by rearranging Eqs. (7)(8)(9)(10):…”

Section: Mass Transfer Model For Backward Extractionmentioning

confidence: 99%

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Kinetics of Protein Extraction in Reverse Micelle

Guo

Chen

Yang

et al. 2015

International Journal of Food Properties

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Sodium bis (2-ethylhexyl) sulfosuccinate (AOT)-sodium dodecyl sulfate (SDS)/isooctane-octanol reverse micelle extraction was tested an efficient and effective approach to separate peanut protein from full-fat peanut powder. Here, important kinetic factors including pH, ion strength, and temperature were studied during reverse micelle backward extraction. The extraction conditions were obtained by response surface experiments as follows: pH 7.5, ion concentration 1.1 mol/L at temperature 35 • C. Under these optimum extraction conditions, the extraction rate of protein reached 79.03%. A model on the kinetic partitioning of peanut protein was also developed. The backward extraction in this reverse micelle system was controlled by interfacial resistance instead of diffusion resistance in reverse micelle and aqueous phase with the total mass transfer rate of 0.8×10 −5 m 3 ·s −1 . A two-film theory may be the mechanism for flat interface. Results of mass transfer process are helpful for creating an reverse micelle extraction process, and used for purification of peanut proteins, promoting the development of food industry.

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“…Dynamics equation is based on the two-film theory, thus the suitability of using this model to reverse micelle system can be obtained by rearranging Eqs. (7)(8)(9)(10):…”

Section: Mass Transfer Model For Backward Extractionmentioning

confidence: 99%

“…[9] The overall mass transfer coefficient K L A can be obtained from the definition of parameters, Eq. (8). Dynamics equation is based on the two-film theory, thus the suitability of using this model to reverse micelle system can be obtained by rearranging Eqs.…”

Section: Mass Transfer Model For Backward Extractionmentioning

confidence: 99%

Kinetics of Protein Extraction in Reverse Micelle

Guo

Chen

Yang

et al. 2015

International Journal of Food Properties

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“…For RMs with spherical morphology, the size of aqueous core depends on the molar ratio of water molecules per surfactant molecule or the hydration degree (w 0 ) [25][26][27]. The micellar system formed by self-assembly of anionic surfactant sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in non-polar solvents has been used to highlight the unique properties of water at the micellar interface [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Self-aggregation of the pyrene labeled poly(acrylic acid) in nanoscopically crowded environments

Stîngă

Băran

Iovescu

et al. 2016

Journal of Photochemistry and Photobiology A: Chemistry

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“…Reverse micelle extraction, a relatively new technology for liquid-liquid extraction, especially for the isolation and purification of proteins, has recently received due attention (Luisi et al, 1979;Harikrishna et al, 2002). Reverse micelles are formed by surfactants in nonpolar organic solvents, while in their polar cores, nanometresized water pools are formed by the solubilisation in water (Luisi et al, 1979;Naoea et al, 2011). With self-assembly capacity, reverse micelle systems are not only dynamic balancing systems but also stable and transparent thermodynamic systems (Liu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Extraction and physicochemical properties of soya bean protein and oil by a new reverse micelle system compared with other extraction methods

Yang

Chen

et al. 2013

Int J of Food Sci Tech

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Summary Reverse micelle extraction is a novel technology for the separation of plant components such as proteins and oil. In this study, sodium bis(2‐ethylhexyl) sulphosuccinate (AOT) reverse micelle system and AOT/Tween 85 reverse micelle system were used to extract soya bean protein and oil from soya bean flour. The physicochemical properties of the protein and oil extracted were investigated and compared with traditional extraction methods. The results showed that the efficiency of forward extraction of soya bean protein using an AOT/Tween 85 reverse micelle system was superior to that using an AOT reverse micelle system at the optimal extraction conditions. In addition, soya bean proteins extracted using reverse micelle extraction had no unordered structure under Fourier transform infrared spectroscopy. The acid and peroxide values of oil products from two reverse micelle extractions were lower than that from immersion. The results indicated that AOT/Tween 85 reverse micelle system is effective in extracting soya bean protein and oil.

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Preparation of protein nanoparticles using AOT reverse micelles

Cited by 17 publications

References 21 publications

Kinetics of Protein Extraction in Reverse Micelle

Kinetics of Protein Extraction in Reverse Micelle

Self-aggregation of the pyrene labeled poly(acrylic acid) in nanoscopically crowded environments

Extraction and physicochemical properties of soya bean protein and oil by a new reverse micelle system compared with other extraction methods

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