Preparation of regenerable magnetic nanoparticles for cellulase immobilization: Improvement of enzymatic activity and stability

Abstract

To obtain regenerable magnetic nanoparticles, triethoxy(3-isocyanatopropyl)silane and iminodiacetic acid (IZ) were used as the starting material and immobilized on Fe 3 O 4 nanoparticles. Copper ions (Cu 2+ ions) were loaded on the Fe-IZ nanoparticles and used for cellulase immobilization. The support was characterized by spectroscopic methods (FTIR, NMR) and thermogravimetric analysis, transmission electron microscopy, scanning electron microscope, X-ray diffraction, energy dispersive X-ray analysis, and vibrating sample magnetometer techniques. As a result of experiments, the amount of protein bound to immobilized cellulase (Fe-IZ-Cu-E) and cellulase activity was found to be 33.1 mg/g and 154 U/g at pH 5, 50 C, for 3 h. The results indicated that the free cellulase had kept only 50% of its activity after 2 h, while the Fe-IZ-Cu-E was observed to be around 77%, at 60 C. It was found that the immobilized cellulase maintained 93% of its initial catalytic activity after its sixth use. Furthermore, the Fe-IZ-Cu-E retained about 75% of its initial activity after 28 days of storage. To reuse the support material (Fe-IZ-Cu), it was regenerated by thorough washing with ammonia or imidazole.

Curcumin extraction from turmeric plant using magnetic Fe3O4 nanoparticles

“…A suitable magnetic field is created by adding biomolecules or organic substances to these magnetic nanoparticles, and the separation of substances can be performed quickly and easily by using magnets. [42,51] For the isolation of curcumin, unlike other purification techniques, the use of magnetic nanoparticles was preferred considering the advantage of a simple and fast separation technique with the help of magnets. In the study, Fe 3 O 4 nanoparticles were synthesized and their structure was confirmed by characterization techniques.…”

“…[38] The prepared magnetic nanoparticles have been widely used in bionanotechnology such as magnetic resonance imaging, bioseparation, diagnostic agents, tumor hyperthermia and biomolecule immobilization. [39][40][41][42][43][44][45][46][47][48][49][50][51] These magnetic nanoparticles have attracted the attention of researchers with their small size, low toxicity, superparamagnetism and most importantly their specific applications. [36,[51][52][53][54][55] In this study, curcumin, which is the miracle of nature, was obtained by purifying it quickly and easily with magnetic iron oxide nanoparticles (IONPs) without the use of expensive instruments.…”

Review on sterilization techniques, and the application potential of phage lyase and lyase immobilization in fighting drug-resistant bacteria

“…100 The enzyme's stability and reusability after immobilization will be significantly improved, and the heterogeneous form of the enzyme will be easily separated from the reaction mixture. 101 Lyase, as an enzyme, is certainly no exception.…”

Enhancing Biogas Production with The Addition of Nano-catalysts

“…Copper (Cu) based nanostructures have been found to have an impact on the stability, immobilization, and recovery of enzymes among a variety of investigated nanomaterials (12). Furthermore, the inclusion of Cu-based nanomaterials may have improved the CAZymes' (carbohydrate-active enzymes) functional activity, which raises the efficiency of cellulose degradation during the enzymatic hydrolysis reaction (13). Platinum-based nanocatalysts can boost watersplitting efficiency.…”