Preparation of silk fibroin carriers for controlled release

Liu, Qiang; Liu, Haifeng; Fan, Yubo

doi:10.1002/jemt.22606

Cited by 40 publications

(22 citation statements)

References 94 publications

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“…To utilize the superior properties of silk stored in its molecular structures, nano‐level formulations of silk can be exploited using various routes. Nanoparticles of silk fibroin can be obtained through emulsion‐solvent evaporation, self‐assembly, phase separation, spray drying, rapid expansion of supercritical solution, electro‐gelation, salting out, and so on . The above procedures utilize reconstituted silk having random coil structures which needs to be reconverted to pleated structures; hence material properties are compromised .…”

Section: Introductionmentioning

confidence: 99%

Silk nano‐discs: A natural material for cancer therapy

et al. 2018

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The article demonstrates the crystalline silk nano‐discs (CSNs), with well‐controlled morphology, which upon magnetization, yields magnetic crystalline silk nano‐discs, making both prominent alternatives for replacing metal templates such as gold, silver, and so on in therapeutics and implants. The isolated β‐sheet‐rich discotic CSNs have ~50 nm diameter, high crystallinity (> 90%), and are insoluble but provide good dispersibility and stability in aqueous solutions. The melt blending‐cum‐electrospinning of functionalized CSN with poly(lactic acid) results in biocompatible nanofiber‐based scaffolds having in vitro cell cytocompatibility with improved cell adhesion and proliferation. The assessment of release behavior of curcumin, a naturally occurring anticancer drug, shows sustained release over 25 days exhibiting effective cytotoxicity against human cervical cancer cells. Further, combined effect of curcumin and hyperthermia reduced the cell growth by ~63%. Alignment of CSN‐derived magnetic nanoparticles due to effective fiber drawing process during electrospinning could improve cytocompatibility against BHK‐21 cells, and therefore efficacy for cancer therapy.

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Section: Introductionmentioning

confidence: 99%

Silk nano‐discs: A natural material for cancer therapy

et al. 2018

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“…Several research studies have shown the excellent characteristics of SFNs as a vehicle for drugs and biomolecules [38][39][40][41], so their production will presumably reach industrial scale in coming years. For this reason, it is of great interest to know all the parameters that affect the formation of nanoparticles to standardize the process and control their features.…”

Section: Introductionmentioning

confidence: 99%

Revealing the Influence of the Degumming Process in the Properties of Silk Fibroin Nanoparticles

Carissimi¹,

Lozano-Pérez²,

Montalbán³

et al. 2019

Preprint

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In recent years, numerous research studies have shown the excellent characteristics of silk fibroin nanoparticles as a vehicle for drugs delivery and it is foreseeable that their production could reach industrial scale in the coming years. For this reason, it is essential to know all the parameters that affect the formation of nanoparticles in order to standardize the process. Several studies have stated that the process used for sericin removal (degumming) from silk cocoons has a strong impact in the silk fibroin integrity and their mechanical properties after processing it into biomaterials. In this work, silk cocoons were degummed following four standard methods: autoclaving, short alkaline (Na2CO3) boiling, long alkaline (Na2CO3) boiling and ultrasounds. The resultant silk fibroin fibers were dissolved in the ionic liquid 1-ethyl-3-methylimidazolium acetate and used for nanoparticle synthesis by rapid desolvation in polar organic solvents. The relative efficiencies of the degumming processes and the integrity of the resulting fibroin fibers obtained were analyzed by weight loss, optical microscopy, thermogravimetric analysis, infrared spectroscopy and SDS-PAGE. Particle sizes and morphology were analyzed by Dynamic Light Scattering and Field Emission Scanning Electronic Microscopy. The results showed that the different treatments had a remarkable impact on the integrity of the silk fibroin chains, as confirmed by gel electrophoresis which can be correlated with particle mean size and size distribution changes. The study confirm that all the parameters of the process must be controlled in order to reach an optimum reproducibility of the nanoparticle production.

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“…Silk fibroin (SF), a fibrous protein extracted from silkworm cocoons, is being explored as a promising material for high‐performance wound dressings and controlled delivery systems . SF membranes are able to absorb wound exudate, becoming flexible dressings, and to adhere to the wound, preventing excessive flow of exudates, proteins, and substances that promote cell proliferation .…”

Section: Introductionmentioning

confidence: 99%

Silk fibroin membranes with self‐assembled globular structures for controlled drug release

Tomoda

Corazza

Beppu

et al. 2019

J of Applied Polymer Sci

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The cover image created by Mariana Agostini de Moraes and colleagues, represents the self-assembling of silk fibroin into globular structures within a continuous fibroin membrane matrix, encapsulating the drug model -diclofenac sodium. These structures were only observed when the drug was incorporated in the fibroin solution, prior to membrane preparation. The formation was spontaneous, and the globular structures presented regular size, shape and uniform distribution within the membrane. EDX mapping (red dots) of a fractured particle confirmed the high concentration of the drug within the globular structures. These results emphasize the potential use of silk fibroin for drug delivery applications.

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Preparation of silk fibroin carriers for controlled release

Cited by 40 publications

References 94 publications

Silk nano‐discs: A natural material for cancer therapy

Silk nano‐discs: A natural material for cancer therapy

Revealing the Influence of the Degumming Process in the Properties of Silk Fibroin Nanoparticles

Silk fibroin membranes with self‐assembled globular structures for controlled drug release

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