Preparation of stable micropatterns of gold on cell-adhesion-resistant hydrogels assisted by a hetero-bifunctional macromonomer linker

Sun, Jianguo; Gräter, Stefan; Tang, Jian; Huang, Jing; Liu, Peng; Lai, Yu Xiao; Majer, G.; Spatz, Joachim P.; Ding, Jian Jun

doi:10.1007/s11426-013-5057-8

Cited by 15 publications

(10 citation statements)

References 49 publications

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“…The method to prepare micropatterns had been reported previously by our group [2–11] . First, gold micropatterns were fabricated on glass with a pre-designed mask through photolithography.…”

Section: Data Experimental Design Materials and Methodsmentioning

confidence: 99%

Data in support of effects of cell–cell contact and oxygen tension on chondrogenic differentiation of stem cells

Cao

Peng

et al. 2015

Data in Brief

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This paper presents data related to the research article entitled “Effects of cell–cell contact and oxygen tension on chondrogenic differentiation of stem cells” [1]. Three sets of micropatterns were fabricated to study the influence of the cell–cell contact on the chondrogenic induction of mesenchymal stem cells (MSCs). The basic repeat units of these micropatterns were of the same area and microisland number to guarantee the same cell density in each culture well. Cells on these micropatterns experienced the same microenvironment except cell–cell contact extent. Immunofluorescent staining and quantitative real-time polymerase chain reaction (qRT-PCR) were performed, and the data are included here.

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Section: Data Experimental Design Materials and Methodsmentioning

confidence: 99%

Data in support of effects of cell–cell contact and oxygen tension on chondrogenic differentiation of stem cells

Cao

Peng

et al. 2015

Data in Brief

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“…46 In cooperation with Spatz's group, our laboratory has established a transfer lithography strategy to fabricate gold and then RGD arrays on PEG hydrogels. 47,48 In this study, hexagonal RGD nanopatterns were fabricated on PEG hydrogels to explore the effects of RGD nanospacing on adhesion and chondrogenic differentiation of MSCs, as schematically presented in Fig. 1A.…”

Section: Introductionmentioning

confidence: 99%

Effects of RGD nanospacing on chondrogenic differentiation of mesenchymal stem cells

Cao

Wang

et al. 2015

J. Mater. Chem. B

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Modification of material surfaces by arginine-glycine-aspartate (RGD) peptide, a ligand of transmembrane integrin, can regulate cell adhesion and other events in tissue engineering and regenerative medicine. While both RGD effects and chondrogenesis are very important and have been much reported, the studies on the influence of the spatial arrangement of RGD ligands on chondrogenic differentiation are rather limited. Herein, we examined the effects of RGD nanospacing on in vitro two-dimensional chondrogenic differentiation of mesenchymal stem cells (MSCs) for the first time. With a unique nanolithography technology, hexagonal RGD nanopatterns were generated on a nonfouling poly(ethylene glycol) (PEG) hydrogel. Two nanospacings (63 and 161 nm) were achieved, with one below and the other above the critical nanospacing. After one-day incubation and nine-day chondrogenic induction, expressions of collagen II proteins and chondrocyte-specific genes (SOX9, aggrecan and collagen II) were detected. The statistics illustrated that the large nanospacing led to a less spreading area and a higher chondrogenic differentiation extent. The further tests by the addition of an inhibitor SB203580 confirmed the positive regulation of p38 phospho-relay cascade on the chondrogenic induction in this model system.

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“…The BRI/PHEMA contact lens was prepared by a UV light polymerization reaction as reported previously [ 9 , 25 ]. Briefly, 6 g of 2-hydroxyethyl methacrylate (HEMA) monomer solution was mixed with 90 mg of brimonidine tartrate, 180 mg of PEG-DMA as a cross linker and 18 mg of D2959 in a 15-mL brown glass bottle and the mixture was gently stirred under N 2 gas for 20 min.…”

Section: Methodsmentioning

confidence: 99%

A validated stability-indicating HPLC method for determination of brimonidine tartrate in BRI/PHEMA drug delivery systems

Sun

Zhang

Huang

2017

Chemistry Central Journal

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BackgroundA simple, rapid and accurate stability-indicating reverse phase high performance liquid chromatography (RP-HPLC) was developed and validated for the determination of brimonidine tartrate in brimonidine tartrate/poly(2-hydroxyethyl methacrylate) (BRI/PHEMA) drug delivery contact lenses and pharmaceutical formulations.ResultsOptimum chromatographic conditions for separating brimonidine tartrate from other impurities in the leaching liquor of BRI/PHEMA drug delivery contact lenses or pharmaceutical formulations have been achieved by using a Diamonsil C18 column (150 mm × 4.6 mm, 5 μm) as a stationary phase and a mixture solution of phosphate buffer (10 mM, pH3.5) containing 0.5% triethlamine and methanol (85:15, v/v) as a mobile phase at a flow rate of 1 mL/min. The theoretical plates for the brimonidine tartrate measurement were calculated to be 8360 when detection was performed at 246 nm using a diode array detector. The proposed method was validated in accordance with ICH guidelines with respect to linearity, accuracy, precision, robustness, specificity, limit of detection and quantitation. Regression analysis showed a good correlation (R2 > 0.999) for brimonidine tartrate in the concentration range of 0.01–50 μg/mL. The peak purity factor is ≥980 for the analyte after all types of stress tests, indicating an excellent separation of brimonidine tartrate peak from other impurities. The measurement course could be completed within 10 min, which was very quick, effective and convenient.ConclusionsOverall, the proposed stability-indicating method was suitable for routine quality control and drug analysis of brimonidine tartrate in BRI/PHEMA drug delivery contact lenses and other pharmaceutical formulations.

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Preparation of stable micropatterns of gold on cell-adhesion-resistant hydrogels assisted by a hetero-bifunctional macromonomer linker

Cited by 15 publications

References 49 publications

Data in support of effects of cell–cell contact and oxygen tension on chondrogenic differentiation of stem cells

Data in support of effects of cell–cell contact and oxygen tension on chondrogenic differentiation of stem cells

Effects of RGD nanospacing on chondrogenic differentiation of mesenchymal stem cells

A validated stability-indicating HPLC method for determination of brimonidine tartrate in BRI/PHEMA drug delivery systems

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