Preparation of Surface-Enhanced Raman Scattering Substrates Based on Immobilized Silver-Capped Nanoparticles

Caro, Carlos; Gámez, Francisco; Zaderenko, Ana Paula

doi:10.1155/2018/4127108

Cited by 27 publications

(10 citation statements)

References 51 publications

(52 reference statements)

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“…The relative standard deviation (RSD) of these EC-SERS intensities was calculated to be 1.41%. Compared with other studies [ 36 , 37 , 38 ], the RSD value obtained here is very low, which indicates that the SERS enhancement of the developed EC-SERS chip is quite uniform and suite for quantitative measurement. In addition, compared with the SERS chip, the integrated EC-SERS chip can directly detect the analyte solution without waiting for it to dry on the SERS chip.…”

Section: Resultscontrasting

confidence: 76%

Integrated EC-SERS Chip with Uniform Nanostructured EC-SERS Active Working Electrode for Rapid Detection of Uric Acid

Huang

Hsiao

2020

Sensors

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Toxemia of pregnancy is a very dangerous disease for pregnant women. The mortality rate of toxemia of pregnancy is close to 10% to 15%. Early detection of pregnancy toxemia is to monitoring uric acid concentration in urine. The current mainstream method for detecting uric acid requires an enzyme (urate oxidase), which needs to be stored in a low-temperature environment, and the method requires complex chemical steps, which takes a longer time and more samples. In this study, we propose an integrated miniature three-electrode electrochemical surface-enhanced Raman spectroscopy chip (EC-SERS chip) suitable for rapid EC-SERS detection applications. The integrated microfluidic reservoir on the chip makes it easy to use, which is very suitable for rapid detection applications. The SERS active working electrode for the proposed integrated EC-SERS chip is a nanocone array polycarbonate (PC) substrate decorated with an evenly distributed and tightly packed array of gold nanospheres. It showed good uniformity and can be easily reproduced. The integrated EC-SERS chip is very small compared to the traditional electrochemical cell, which reduces the sample volume required for the testing. In addition, the chip is for one-time use only. It eliminates the need to clean electrochemical cells for reuse, thereby reducing the possibility of contamination and inaccurate detection. Various low-concentration Rhodamine 6G (R6G) solutions were tested to verify the performance of the developed EC-SERS chip. Experimental results showed that the proposed EC-SERS chip has a strong enhancement factor of up to 8.5 × 106 and a very good EC-SERS uniformity (the relative standard deviation of EC-SERS intensity is as low as 1.41%). The EC-SERS chip developed has been further tested for the detection of uric acid in synthetic urine. The results showed that the EC-SERS signal intensity has a highly linear relationship with the logarithm of the uric acid concentration in synthetic urine, which indicates that the developed EC-SERS chip is suitable for the quantitative detection of uric acid in synthetic urine. Therefore, the developed EC-SERS chip is very promising to be used in routine and early diagnosis of pregnancy toxemia and may be used in many other medical tests, food safety, and biotechnology applications.

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Section: Resultscontrasting

confidence: 76%

Integrated EC-SERS Chip with Uniform Nanostructured EC-SERS Active Working Electrode for Rapid Detection of Uric Acid

Huang

Hsiao

2020

Sensors

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“…relative standard deviation (RSD) of intensities of the 1503 cm −1 Raman characteristic peak was calculated to be 7.28%, which is relatively low compared to other studies [25][26][27], indicating good uniformity and reproducibility of the fabricated SERS substrates. Internsity (a.u.…”

Section: Performance Of the Sers Substratesmentioning

confidence: 61%

“…The SERS intensities of the Raman characteristic peak of the 1503 cm −1 band from these locations are further displayed in Figure 10b. The relative standard deviation (RSD) of intensities of the 1503 cm −1 Raman characteristic peak was calculated to be 7.28%, which is relatively low compared to other studies [25][26][27], indicating good uniformity and reproducibility of the fabricated SERS substrates. characteristic peak of the 1503 cm band from these locations are further displayed in Figure 10b.…”

Section: Performance Of the Sers Substratesmentioning

confidence: 63%

Tunable Silver Nanoparticle Arrays by Hot Embossing and Sputter Deposition for Surface-Enhanced Raman Scattering

Huang

Tsai

2019

Applied Sciences

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Surface-enhanced Raman scattering (SERS) spectroscopy has attracted a lot of attention over the past 30 years. Due to its extreme sensitivity and label-free detection capability, it has shown great potential in areas such as analytical chemistry, biochemistry, and environmental science. However, the major challenge is to manufacture large-scale highly SERS active substrates with high controllability, good reproducibility, and low cost. In this study, we report a novel method to fabricate uniform silver nanoparticle arrays with tunable particle sizes and interparticle gaps. Using hot embossing and sputtering techniques, we were able to batch produce the silver nanoparticle arrays SERS active substrate with consistent quality and low cost. We showed that the proposed SERS active substrate has good uniformity and high reproducibility. Experimental results show that the SERS enhancement factor is affected by silver nanoparticles size and interparticle gaps. Furthermore, the enhancement factor of the SERS signal obtained from Rhodamine 6G (R6G) probe molecules was as high as 1.12 × 107. Therefore, the developed method is very promising for use in many SERS applications.

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“…Recently, various metal nanoparticles with different shapes have provided unique plasmonic behavior to construct SERS-based biosensors [ 23 ]. For example, it was reported that through an enhancement of Au nanorods a Raman signal could be elucidated by longitudinal and transverse plasmon bands, an effect which had not been observed in spherical Au nanoparticles [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Bioprobe Self-Assembled on Au–Te Nanoworm Structure for SERS Biosensor

et al. 2020

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In the present study, we propose a novel biosensor platform using a gold-tellurium (Au–Te) nanoworm structure through surface-enhanced Raman spectroscopy (SERS). Au–Tenanoworm was synthesized by spontaneous galvanic replacement of sacrificial Te nanorods templated with Au (III) cations under ambient conditions. The fabricated Au–Te nanoworm exhibited an interconnected structure of small spherical nanoparticles and was found to be effective at enhancing Raman scattering. The Au–Te nanoworm-immobilized substrate exhibited the ability to detect thyroxine using an aptamer-tagged DNA three-way junction (3WJ) and glycoprotein 120 (GP120) human immunodeficiency virus (HIV) using an antibody. The modified substrates were investigated by scanning electron microscopy and atomic force microscopy (AFM). The optimal Au–Te nanoworm concentration and immobilization time for the thyroxine biosensor platform were further determined by SERS experimentation. Thus, the present study showed that the Au–Te nanoworm structure could be applied to various biosensor platforms.

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Preparation of Surface-Enhanced Raman Scattering Substrates Based on Immobilized Silver-Capped Nanoparticles

Cited by 27 publications

References 51 publications

Integrated EC-SERS Chip with Uniform Nanostructured EC-SERS Active Working Electrode for Rapid Detection of Uric Acid

Integrated EC-SERS Chip with Uniform Nanostructured EC-SERS Active Working Electrode for Rapid Detection of Uric Acid

Tunable Silver Nanoparticle Arrays by Hot Embossing and Sputter Deposition for Surface-Enhanced Raman Scattering

Fabrication of Bioprobe Self-Assembled on Au–Te Nanoworm Structure for SERS Biosensor

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