Preparation of three-dimensional interconnected macroporous cellulosic hydrogels for soft tissue engineering

Yue, Zhilian; Wen, Feng; Gao, Shujun; Ang, Ming Yi; Pallathadka, Pramoda K.; Liu, Lihong; Yu, Hanry

doi:10.1016/j.biomaterials.2010.07.059

Cited by 47 publications

(37 citation statements)

References 41 publications

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“…Simultaneously, we prepared a suitable bio-ink for dissociated primary cortical neural cells based on peptide-modified GG that was compatible with this printing process. Scanning electron microscopy was used as previously reported to probe the internal structure of the printed hydrogels [51,52]. Fluorescence staining and Confocal microscopy were employed to determine the cell viability, phenotype and morphology of neural networks.…”

Section: Introductionmentioning

confidence: 99%

3D printing of layered brain-like structures using peptide modified gellan gum substrates

et al. 2015

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Section: Introductionmentioning

confidence: 99%

3D printing of layered brain-like structures using peptide modified gellan gum substrates

et al. 2015

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“…Among the many hydrogels studied, those based on natural cellulose and its derivatives have attracted particular attention due to their adequate biocompatibility and biodegradability 6. Cellulose based hydrogels have been proven to be useful in tissue engineering, wound healing, and tissue regeneration 7–10…”

Section: Introductionmentioning

confidence: 99%

Determination of crosslinking density of hydrogels prepared from microcrystalline cellulose

Xia

Patchan

Maranchi

et al. 2012

J of Applied Polymer Sci

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Hydrogels with four different plant-based microcrystalline cellulose concentrations were prepared using the self-assembly technique. The interaction parameter between cellulose and water was determined by the classical Flory-Huggins theory, and was found to be around 0.44 with weak concentration dependence. The crosslinking density in these hydrogels was measured by both the Mooney-Rivlin equation and the Flory-Rehner theory. Reasonable consistency was found between the two methods albeit results from the Flory-Rehner theory were slightly higher due to the contribution from the physical crosslinks. The crosslinking density values for all four hydrogels determined from both methods were found to range from 19 to 56 mol/m 3 .

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“…As a result, the duration and/or severity of the inflammation occurring due to activation of various systems for scar healing at the cellular level is accepted as the biocompatibility. [19][20][21][22] While evaluating biomaterials, most of the times the first target is to evaluate their physical and mechanical properties while biological properties are not treated the same. As any material that is in contact with tissue or blood poses a potential risk for health by causing some reactions, the advantages and disadvantages of such materials should be taken into consideration before clinical application, and their biocompatibilities should be evaluated by cytotoxicity tests.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the cytotoxicity of ventilation tube, nasal packing, and spongostan used in ear-nose-throat surgeries

Altuntaş

Sümer

2011

Indian J Otol

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important step in evaluating biocompatibility is choosing the appropriate test method. [5][6][7][8][9] Although we found some animal studies in literature evaluating biocompatibility of spongostan, merocell, and ventilation tube used in ear-nose-throat surgeries and investigating whether they have any negative effects on the success rate of surgery, we could not find any cytotoxicity studies carried out with cell culture and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT test) test. [10][11][12][13] The MTT test is widely used in the assessment of drugs and chemicals, but is less well accepted in the study of toxicity of AbstrAct Purpose: The aim of this study was to determine biocompatibility of spongostan, merocell, and ventilation tube using agar diffusion and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide tests in cell culture and to evaluate the results in terms of clinical practices. Materials and Methods: The experimental procedures involved in this study were approved by the Animal Ethics Committee of Cumhuriyet University (B.30.2.CUM.0.01.00-50/4), and the study was conducted following accepted guidelines for the care and use of laboratory animals for research. The present study was supported by Cumhuriyet University Scientific Research Projects (CÜBAP) Project no T-408. L929 mouse fibroblast cell culture was used in the present study. In this study, indirect toxic effects of the leakage products from the materials were tested with the agar overlay test, and also direct toxic effects of leakage materials occurring at different time and leakage products from the materials were tested with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide test. Results: As a result of evaluating the 21 days' leakage material of the ventilation tube a moderate (+2), 24 and 48 h of leakage material of the merocell low degree and 24-hour leakage material of the spongostan limited cytotoxicity were found. Conclusion: Many biomaterials cytotoxicity study were found in the literature as well as in our study shown by MTT and agar diffusion tests they were not toxic.

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Preparation of three-dimensional interconnected macroporous cellulosic hydrogels for soft tissue engineering

Cited by 47 publications

References 41 publications

3D printing of layered brain-like structures using peptide modified gellan gum substrates

3D printing of layered brain-like structures using peptide modified gellan gum substrates

Determination of crosslinking density of hydrogels prepared from microcrystalline cellulose

Evaluation of the cytotoxicity of ventilation tube, nasal packing, and spongostan used in ear-nose-throat surgeries

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