Preparation of Water-Soluble Hyperbranched Polyester Nanoparticles with Sulfonic Acid Functional Groups and Their Micelles Behavior, Anticoagulant Effect and Cytotoxicity

Abstract

Biocompatibility of nanoparticles has been attracting great interest in the development of nanoscience and nanotechnology. Herein, the aliphatic water-soluble hyperbranched polyester nanoparticles with sulfonic acid functional groups (HBPE-SO3 NPs) were synthesized and characterized. They are amphiphilic polymeric nanoparticles with hydrophobic hyperbranched polyester (HBPE) core and hydrophilic sulfonic acid terminal groups. Based on our observations, we believe there are two forms of HBPE-SO3 NPs in water under different conditions: unimolecular micelles and large multimolecular micelles. The biocompatibility and anticoagulant effect of the HBPE-SO3 NPs were investigated using coagulation tests, hemolysis assay, morphological changes of red blood cells (RBCs), complement and platelet activation detection, and cytotoxicity (MTT). The results confirmed that the sulfonic acid terminal groups can substantially enhance the anticoagulant property of HBPE, and the HBPE-SO3 NPs have the potential to be used in nanomedicine due to their good bioproperties.

Heparin mimetics with anticoagulant activity

“…Besides linear synthetic sulfonated polymers, other shaped polymers such as hyperbranched or dendritic polymers prepared from sulfonated monomers have been reported to act as anticoagulants. For example, hyper‐branched sulfonated polyester nanoparticles inhibited both intrinsic and/or common pathways and thrombin activity or fibrin formation from fibrinogen . Alban's group prepared tree‐like structured dendritic polyglycerol sulfate whose anticoagulant activity does not depend on the MW due to the globular 3D structure …”

Fabrication of Fe3O4 nano-aggregates hybrid material via self-assembly of nanocapsules based on amphiphilic hyperbranched polyglycerols

“…The bands at 2925.8 cm À 1 , 2856.4 cm À 1 and 723.1 cm À 1 were attributed to the stretching and asymmetrical vibration of C-H that belong to long alkene, which were consistent with the corresponding bands of oleic acid (curve b), confirming the oleic acid was introduced into HPG by esterification. Moreover, comparing with HPG (curve a), the -OH stretching vibration bands of AP-HPG at 3444.8 cm À 1 turned wider and shifted to higher wavenumber, suggesting the original intermolecular hydrogen bonding in HPG was broken during esterification [7]. The results indicate AP-HPG was synthesized based on HPG with terminal hydroxyl groups modified with oleic acid.…”

“…The topological encapsulation of HBPs for various guests has attracted significant attention because of their internal cavities and better stability compared with the linear analogue [3][4][5]. Especially, amphiphilic hyperbranched polymers (AP-HBPs) with core-shell structures have shown micelle-like properties that have led to the picture of a "unimolecular micelle", which show much better encapsulation performance compared with classical micelles [5][6][7]. To date, AP-HBPs have been mainly used in diverse fields such as drug and gene delivery [7,8], phase-transfer agent [9] and nanoreactor [3].…”

“…Especially, amphiphilic hyperbranched polymers (AP-HBPs) with core-shell structures have shown micelle-like properties that have led to the picture of a "unimolecular micelle", which show much better encapsulation performance compared with classical micelles [5][6][7]. To date, AP-HBPs have been mainly used in diverse fields such as drug and gene delivery [7,8], phase-transfer agent [9] and nanoreactor [3]. It is noticed that most researches of AP-HBPs exhibit the aggregation-free in solution because of the amphiphilic nature, which are the unique supramolecular self-assembly behaviors belonging to AP-HBPs.…”

Inulin-d-α-Tocopherol Succinate (INVITE) Nanomicelles as a Platform for Effective Intravenous Administration of Curcumin

“…A well-established method to evaluate blood compatibility of micellar formulations intended for intravenous administration, is the hemolysis assay performed on whole blood. 22,51,52 This test evaluates the release of hemoglobin from the red blood cells (RBCs) in the case of cellular rupture upon contact with a foreign material.…”