In this study, we have used Zizyphus mauritiana (ZM) extracts and chloroauric acid (HAuCl 4) for synthesis of gold nanoparticles, with a particle size ranging from 20 to 40 nm. The ZM extract, acts both as reducing and stabilizing agent. UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infra red spectroscopy (FTIR), transmission electron microscopy, scanning electron microscopy (SEM), and X-ray energy dispersive spectrophotometer were performed to ascertain the formation of Au-NPs. It was observed that the growths of Au-NPs are stopped within 25 min of reaction time. The synthesized Au-NPs were characterized by a peak at 535 nm in the UV-visible spectrum. XRD confirmed the crystalline nature of the nanoparticles of 27 nm size. The FTIR result clearly showed that the extracts containing OH as a functional group act in capping the nanoparticles synthesis. Antibacterial activities of Au-NPs were tested against the growth of Gram-positive (S. aureus) using SEM. Therefore, green synthesis of gold nanoparticles with ZM extract, is beneficial from its biological and medical applications.