Preparation of31Si-labelled silicate: a radiotracer for silicon studies in biosystems

Brasser, H. J.; Gurboga, G.; Kroon, J. J.; Kolář, Z.; Wolterbeek, Hubert Th.; Volkers, K. J.; Krijger, Gerard C.

doi:10.1002/jlcr.1096

Cited by 2 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All chemicals used in this study were of analytical grade, obtained from Aldrich (Zwijndrecht, The Netherlands 4 solution (specific activity 4.8 TBq/g) was prepared as described earlier [32], and was successfully used earlier in Si uptake studies in yeast [13].…”

Section: Chemicals For Radioactivity Studiesmentioning

confidence: 99%

Compartmental Analysis Suggests Macropinocytosis at the Onset of Diatom Valve Formation

Brasser

Strate

Gieskes

et al. 2010

Silicon

Self Cite

View full text Add to dashboard Cite

During valve formation of the siliceous frustules of diatoms, bulk uptake of silicic acid and its subsequent transport through the cell is required before it can be deposited in the silica deposition vesicle (SDV). It has been assumed that transport takes place via silicon transporters (SITs), but if that were the case a control mechanism would have to exist for stabilization of the large amounts of reactive silicon species during their passage through the cell on the way to the SDV. There is, however, no reason to assume that classical silica chemistry does not apply at elevated levels of silicic acid, and therefore autopolymerization could reasonably be expected to occur. In order to find alternative ways of Si transport that correspond with the high speed of valve formation at the earliest stages of cell division we followed 31 Si(OH) 4 uptake in synchronously dividing cells of the diatoms Coscinodiscus wailesii, Navicula pelliculosa, N. salinarum, and Pleurosira laevis.The results were related to systematically derived mathematical models for a compartmental analysis of 5 possible uptake/transport pathways, including one involving SITs and one involving (macro)pinocytosis-mediated uptake from the extracellular environment. Our study indicates that the uptake of radioactive silicic acid matches best with the model that describes macropinocytosis-mediated silicon uptake. This process is well in line with the observed 'surge uptake' at the start of valve formation when the demand for silicon is high; it infers that in diatoms a pathway of uptake and transport exists in which SITs are not involved.

show abstract

Section: Chemicals For Radioactivity Studiesmentioning

confidence: 99%

Compartmental Analysis Suggests Macropinocytosis at the Onset of Diatom Valve Formation

Brasser

Strate

Gieskes

et al. 2010

Silicon

Self Cite

View full text Add to dashboard Cite

show abstract

“…31 Si-silicate (t 1/2 2.62 h, β -1.49 MeV) was produced in the nuclear reactor of the Reactor Institute Delft, University of Technology Delft, The Netherlands. No-carrier-added 31 Si-silicate solution (specific activity 4.8 TBq/g) was prepared by purification using a chemical precipitation reaction with barium carbonate as described earlier [27].…”

Section: Chemicals and Labwarementioning

confidence: 99%

On the Beneficial Role of Silicon to Organisms: A Case Study on the Importance of Silicon Chemistry to Metal Accumulation in Yeast

Brasser

Krijger

Wolterbeek

2008

Biol Trace Elem Res

Self Cite

View full text Add to dashboard Cite

Silicon is involved in numerous important structural and functional roles in a wide range of organisms, including diatoms, plants, and humans, but clear mechanisms have been discovered only in diatoms and sponges. Silicate availability influences metal concentrations within various cell-and tissue-types, but a mechanism has not been discovered so far. In an earlier study on Baker's yeast Saccharomyces cerevisiae it was proposed that a chemical mechanism, rather than a biological one, is important. In the present study, the interaction of silicon with Baker's yeast is further investigated by studying the influence of zinc and magnesium on Si accumulation both at a low and a high silicate concentration in the medium. Si accumulation fitted well with Freundlich adsorption and Si release followed depolymerization kinetics, indicating that silicate adsorbs to the surface of the cell rather than being transported over the cell membrane. Subsequently, adsorbed silicate interacts with metal ions and, therefore, alters the cell's affinity for these ions. Since several metals are nutritional, these Si interactions can significantly change the growth and viability of organisms. In conclusion, the results show that chemistry is important in Si and metal accumulation in Baker's yeast, and suggest that similar mechanisms should be studied in detail in other organisms to unravel essential roles of Si.

show abstract

Preparation of31Si-labelled silicate: a radiotracer for silicon studies in biosystems

Cited by 2 publications

References 22 publications

Compartmental Analysis Suggests Macropinocytosis at the Onset of Diatom Valve Formation

Compartmental Analysis Suggests Macropinocytosis at the Onset of Diatom Valve Formation

On the Beneficial Role of Silicon to Organisms: A Case Study on the Importance of Silicon Chemistry to Metal Accumulation in Yeast

Contact Info

Product

Resources

About