Preparation, properties and potential applications of exopolysaccharides from bacteria of the genera Xanthobacter and Ancylobater

Кичемазова, Н. В.; Бухарова, Е. Н.; Selivanov, N. Yu.; Bukharova, I. A.; Karpunina, L. V.

doi:10.1134/s0003683817030073

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Therefore, recent alternatives have incorporated a variety of signal amplification approaches such as nanomaterials,80,103–106 enzymes,107 electrochemical108 or electrochemiluminescent109 transduction fashion to detect target miRNAs with both high selectivity and high sensitivity. In 2017, Xiang and colleagues reported a biodegradable MnO 2 NS-based hybridization chain reaction (HCR) strategy to determine miRNA expression even at exceedingly low levels in living cells 110. They designed two hairpins which were separately labeled with the organic dyes FAM (as a FRET donor) and Tamra (TMR, as a FRET acceptor) and loaded onto MnO 2 NSs.…”

Section: Biomedical Applications Of Mno2 Nanosheets (Mno2 Nss)mentioning

confidence: 99%

<p>Manganese dioxide nanosheets: from preparation to biomedical applications</p>

Hou

Dong

et al. 2019

IJN

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Advancements in nanotechnology and molecular biology have promoted the development of a diverse range of models to intervene in various disorders (from diagnosis to treatment and even theranostics). Manganese dioxide nanosheets (MnO 2 NSs), a typical two-dimensional (2D) transition metal oxide of nanomaterial that possesses unique structure and distinct properties have been employed in multiple disciplines in recent decades, especially in the field of biomedicine, including biocatalysis, fluorescence sensing, magnetic resonance imaging and cargo-loading functionality. A brief overview of the different synthetic methodologies for MnO 2 NSs and their state-of-the-art biomedical applications is presented below, as well as the challenges and future perspectives of MnO 2 NSs.

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Section: Biomedical Applications Of Mno2 Nanosheets (Mno2 Nss)mentioning

confidence: 99%

<p>Manganese dioxide nanosheets: from preparation to biomedical applications</p>

Hou

Dong

et al. 2019

IJN

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“…The bacterial genera that dominated during phases I and II, that is, Acinetobacter , Xanthobacter and Agrobacterium continued to dominate the bacterial population in phase III. Acinetobacter calcoaceticus has been found at 200–250 μm beneath the surface of aerobic granules with an extracellular polymeric substances layer protecting them against phenol toxicity (Adav & Lee, 2008), while Xanthobacter produces extracellular polymeric substances and contributes to the formation of more compact and stable granules (Kichemazova et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Microbial community dynamics during aerobic granulation in a sequencing batch reactor (SBR)

Gómez-Basurto

Vital‐Jácome

Gómez-Acata

et al. 2019

PeerJ

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Microorganisms in aerobic granules formed in sequencing batch reactors (SBR) remove contaminants, such as xenobiotics or dyes, from wastewater. The granules, however, are not stable over time, decreasing the removal of the pollutant. A better understanding of the granule formation and the dynamics of the microorganisms involved will help to optimize the removal of contaminants from wastewater in a SBR. Sequencing the 16S rRNA gene and internal transcribed spacer PCR amplicons revealed that during the acclimation phase the relative abundance of Acinetobacter reached 70.8%. At the start of the granulation phase the relative abundance of Agrobacterium reached 35.9% and that of Dipodascus 89.7% during the mature granule phase. Fluffy granules were detected on day 43. The granules with filamentous overgrowth were not stable and they lysed on day 46 resulting in biomass wash-out. It was found that the reactor operation strategy resulted in stable aerobic granules for 46 days. As the reactor operations remained the same from the mature granule phase to the end of the experiment, the disintegration of the granules after day 46 was due to changes in the microbial community structure and not by the reactor operation.

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Phisical and chemical properties of exopolysaccharide of the lactic streptococcus

Fokina

Uryadova

Karpunina

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

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We know that exopolysaccharides of microbial origin are of great practical importance, and have their industrial value because of the biological and physico - chemical characteristics of the producer. We had a goal to study the most important properties of an exopolysaccharide isolated from a bacterial culture of Streptococcus thermophilus. First we got exopolysaccharide. After that we had cultured the bacteria on A. Welman at 38 °C for 48 hours. To do this, 3000 g of culture liquid had been centrifuged for 30 minutes. Next, we removed the biomass sludge, and we evaporated the centrifuge on a rotary evaporator. After that, we precipitated the exopolysaccharide with a double volume of 96% ethyl alcohol. We got a concentrate which we then dissolved in a small amount of distilled water and had centrifuged for 30 minutes. Afterwards my colleges and I precipitated it again. Our further purification of the exopolysaccharide we had to perform using some gel filtration on a column with a Sephadex G-50. It had to be dried on a freeze dryer. Thus, we obtained an exopolysaccharide in the form of a light brown powder, it was odorless, without any foreign impurities and any producer cells. Our further work included following steps: we had to know the molecular weight of the exopolysaccharide. It was possible to make with gel chromatography on a Toyopearl – HW –50F column. We determined the chemical nature of the exopolysaccharide through ion exchange chromatography using a SPS Bio DEA medium with 70 microns. We determined the monosaccharide composition with the help of a thin-layer chromatography on DC-Alufolien Cellulose plates, and the relative viscosity by viscometer. As a result of our research, the exopolysaccharide S. thermophilus were presented by a single neutral fraction, with molecular weight of 20,000 Da, with a small relative viscosity.

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Preparation, properties and potential applications of exopolysaccharides from bacteria of the genera Xanthobacter and Ancylobater

Cited by 3 publications

References 10 publications

<p>Manganese dioxide nanosheets: from preparation to biomedical applications</p>

<p>Manganese dioxide nanosheets: from preparation to biomedical applications</p>

Microbial community dynamics during aerobic granulation in a sequencing batch reactor (SBR)

Phisical and chemical properties of exopolysaccharide of the lactic streptococcus

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