Preparation, Purification and &lt;i&gt;in vitro&lt;/i&gt; Properties of a Serum against Human Lymphoblastic Leukemia-Associated Antigens

Four hematopoietic cell lines (CCRF-CEM, HSB-2, MOLT-4, and RPMI-8402), derived from acute lymphoblastic leukemia and expressing T-cell surface markers (T-HCL), were studied with two specific anti-T-cell sera. The sera were raised in rabbits against human thymocytes (anti-HTY) and against T-cell cultured in the presence of conditioned medium derived from lymphocytes stimulated with PHA (anti-CTC). Both sera were absorbed to obtain a T-cell specific pattern of reaction and were further absorbed with normal peripheral blood lymphocytes or with each of the four T-HCL. The anti-HTY sera absorbed with CEM, 8402, and HSB-2 still reacted with MOLT-4. A similar pattern of reactivity was found only with the anti-CTC absorbed with 8402, whereas, after absorptions with the other cell lines, this antiserum was unreactive against MOLT-4. After absorption with normal peripheral blood lymphocytes, anti-HTY still reacted with thymocytes and MOLT-4 but was negative on CTC. In contrast, anti-CTC absorbed with peripheral blood lymphocytes (PBL) was negative on thymocytes and MOLT-4 but still reacted against CTC. Our data confirm the existence of a T-cell antigen (probably an early T-cell differentiation antigen) shared between thymus and MOLT-4. This antigen is not expressed on CTC, although these cells express an antigenic pattern more complex than PBL. Antisera to CTC represents a source of anti-T cell sera free of contamination with antibodies to early thymus-related antigens but containing other T-cell-related specificities.

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Immunological studies on xenogeneic antisera against human O-ALL and T-ALL leukemia cells

Milleck¹,

Thränhardt²,

Zintl³

et al. 1980

European Journal of Cancer (1965)

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Preparation, Purification and <i>in vitro</i> Properties of a Serum against Human Lymphoblastic Leukemia-Associated Antigens

Cited by 4 publications

References 7 publications

Expression of T and B Cell Markers and Leukemia-Associated Antigens on Cell Surface of Human Lymphocytic Leukemias

Expression of T and B Cell Markers and Leukemia-Associated Antigens on Cell Surface of Human Lymphocytic Leukemias

Lack of expression on cultured human T-lymphocytes of a T-cell antigen shared by the molt-4 cell line and normal human thymocytes

Immunological studies on xenogeneic antisera against human O-ALL and T-ALL leukemia cells

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