The adsorbent of expanded perlite (EP) was investigated to determine the removal effect on methylene blue (MB) from wastewater through static adsorption. The factors, such as adsorbent dosage, shaking time, temperature, and pH, were investigated. The optimum dosage, shaking time, temperature, and pH was 0.80 g, 100 min, 20 ℃, and 5.67, respectively. Isotherm analysis showed that the patterns of MB adsorption onto expanded perlite was not in line with two adsorption isotherm models, namely, Langmuir and Freundlich. The kinetic characteristics of adsorption were also evaluated. The adsorption process of the MB onto EP was in accordance with the pseudo second-order rate equation. Under ideal experimental parameters, the MB removal efficiency was 94.12% for EP, which indicated that EP is a potential adsorbent for the treatment of MB wastewater.