Preparation Techniques for Transmission Electron Microscopy

Barna, Á.; Radnóczi, G.; Pécz, B.

doi:10.1002/9783527619283.ch18

Handbook of Microscopy Set 1996

DOI: 10.1002/9783527619283.ch18

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Preparation Techniques for Transmission Electron Microscopy

Á. Barna

G. Radnóczi

B. Pécz

Abstract: iii) Diffuse (covering) replicas of C + Pt.(iv) Extraction replicas.Except for technique (ii), these techniques damage the sample being investigated, usually by dissolution. Technique (ii) may preserve the sample, as only a plastic imprint is removed from its surface.

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2023

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“…Morphological features of bacterial cells were determined using an electron microscope Tesla BS 540 (Czech Republic) at an instrumental magnifi cation of × 16,000 [12]. Bacterial cells were tested by the method of negative contrast enhancement with uranyl acetate.…”

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confidence: 99%

Symbiotic Efficiency and Cytokinin Activity of New Mesorhizobium cicerі Strains

Lohosha¹,

Vorobei²,

Leonova

2023

Mikrobiol. Z.

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The efficiency of the introduction of nodule bacteria, microsymbionts of legumes in agrocenoses, largely depends on the activity of biologically active substances’ biosynthesis by diazotrophs. Seed bacterization with effective rhizobia strains capable of synthesizing exometabolites for phytostimulating activity not only promotes the formation and functioning of symbiosis but also creates the conditions for increasing plant resistance to adverse environmental conditions. The aim of the work was to research the symbiotic activity, efficiency and ability of chickpea rhizobia new strains to biosynthesize phytohormonal exometabolites of cytokinin nature. Methods. Microbiological, physiological, cytological, biochemical, and physicochemical. Results. New strains of Mesorhizobium ciceri ND-101 and Mesorhizobium ciceri ND-64 were shown to have different symbiotic activity. The efficiency of inoculation of Skarb chickpea seeds with bacterial suspension of Mesorhizobiu mciceri ND-101 was at the same level with the industrial strain of Mesorhizobium ciceri H-12. Bacterization of Mesorhizobium ciceri ND-64 increased the chickpea roots nodules by 69%, their weight by 74%, and nitrogenase activity by 73% relative to the positive control (inoculation with Mesorhizobium ciceri H-12), as well as increased chickpeas yield by 22%. It was established that Mesorhizobium ciceri ND-64 strain exhibits the highest cytokinin activity in the bioassay. Cytokinins in the total amount of 174.94 μg/g of completely dry biomass were detected in the culture medium of Mesorhizobium ciceri ND-64, which is 53% higher than that of Mesorhizobium ciceri ND-101 strain and 99% higher than that of Mesorhizobium ciceri H-12 strain. Conclusions. Mesorhizobium ciceri ND-64 strain with high nitrogen-fixing activity and symbiotic efficiency is capable to synthesize a relatively high amount of extracellular cytokinins. The high concentration of cytokinins indicates their important role in the formation and functioning of nodules, as they stimulate the proliferation of root tissues and, in this way, have a positive effect on the chickpea productivity.

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confidence: 99%

Symbiotic Efficiency and Cytokinin Activity of New Mesorhizobium cicerі Strains

Lohosha¹,

Vorobei²,

Leonova

2023

Mikrobiol. Z.

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Preparation Techniques for Transmission Electron Microscopy

Abstract: iii) Diffuse (covering) replicas of C + Pt.(iv) Extraction replicas.Except for technique (ii), these techniques damage the sample being investigated, usually by dissolution. Technique (ii) may preserve the sample, as only a plastic imprint is removed from its surface.

Cited by 1 publication

References 99 publications

Symbiotic Efficiency and Cytokinin Activity of New Mesorhizobium cicerі Strains

Symbiotic Efficiency and Cytokinin Activity of New Mesorhizobium cicerі Strains

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