Preparative isolation and purification of theaflavins and catechins by high-speed countercurrent chromatography

Wang, Kunbo; Z, Liu; Huang, Jianan; Dong, Xianlin; Song, Lubing; Pan, Yu; Liu, Fang

doi:10.1016/j.jchromb.2008.04.005

Cited by 42 publications

(27 citation statements)

References 24 publications

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“…However in our hands, best HSCCC separations were obtained at much lower concentrations of the TF sample (150 -200 mg) dissolved in 5 ml of the aqueous mobile phase. Wang et al (2008)used a 30 mg sample of crude TF in 5 ml of the mobile phase while the solvent system used for HSCCC was hexane-ethylacetate-methanol water-acetic acid (1:5:1:5:0.25 v/v). The weights of three fractions obtained in TF separation 1 were F-1 (9 mg), F-2 (11 mg) and F-3 (1.4 mg).…”

Section: Hsccc Separation Of Tfsmentioning

confidence: 99%

“…Commercially available standard samples of TFs [ Cao et al, 2004),TFs from commercial samples of fermented oolong and black tea (Yanagida et al, 2006) and TFs prepared by the enzymic oxidation of tea catechins using polyphenol oxidase isolated from pear fruit ( Wang et al, 2008) havebeen separated using HSCCC. A combination of HSCCC and Sephadex LH-20 chromatography was used by Yang et al to separate TFs from a sample of black tea ( Yang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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The hexane-ethyl acetate-methanol-water system for the separation of theaflavins from black tea (Camellia sinensis) using high-speed counter-current chromatography

2016

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Theaflavins (TFs) from black tea leaves were separated using high-speed countercurrent chromatography (HSCCC). Isobutyl methyl ketone (4-methyl-2-pentanone) was used to separate the TFs from a hot water extract of black tea leaves. Three different compositions of the biphasic solvent system hexane-ethyl acetate-methanol-water (2:5:2:5 solvent system A) 1:4:1:4 (solvent system B) and 1:5:1:5 (solvent system C) were studied. The aqueous phase was used as the mobile phase in the head to tail elution mode. Flow rates of 2.0 -2.8 mL min -1 , revolution speeds of 800-1000 rpm and a 200 mg sample of TF in 5 mL of the aqueous phase with settling times 17-18 secs (solvent systems B and C) gave the best separations. Three peaks were separated in the HSCCC traces obtained using solvent systems B and C, and each peak was monitored by high performance liquid chromatography (HPLC).Separation of theaflavin monogallates was not achieved, but solvent system Cresulted in a complete separation of theaflavin and theaflavin digallate.

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Section: Hsccc Separation Of Tfsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The hexane-ethyl acetate-methanol-water system for the separation of theaflavins from black tea (Camellia sinensis) using high-speed counter-current chromatography

2016

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“…Catechins and OPCs have proved to have biological activities such as protection of cardiovascular disease and the oxygen free radical scavenger capacity, anticancer, antitumor, anti-allergy, improving diabetic conditions, etc. [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…Without stationary phase as solid support, HSCCC can avoid high requirement of sample pretreatment resulting from solid carrier, as well as sample loss and sample denaturation caused by irreversible adsorption, which make separation more efficient [3,10]. HSCCC has been widely used in preparation and separation of natural products.…”

Section: Introductionmentioning

confidence: 99%

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Preparative isolation of monomer catechins and oligomer procyanidin fractions from grape seed extracts by high-speed counter-current chromatography

Zhang

Cui

Sun

2014

BIO Web of Conferences

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Abstract. In this paper, monomer (+)-catechin; (-)-epicatechin and (-)-epicatechin-3-o-gallate, three oligomeric procyanidin fractions (OPCs) and one polymeric procyanidin fractions (PPCs) were successfully separated and isolated from grape seed extracts using semi-preparative TBE-300 B high-speed counter-current chromatography (HSCCC). For their separation in monomer (+)-catechin; (-)-epicatechin and (-)-epicatechin 3-O-gallate, hexane/ethyl acetate/water (1:10: 10, v/v/v) was selected as solvent system. For separation of OPCs and PPCs, solvent system ethyl acetate/water (1:1, v/v) was selected. Compositional data and mean polymerization degrees of OPCs were determined by phloroglucinolysis method. The purity of each fraction obtained; monomer (+)-catechin (C), (-)-epicatechin (EC) and (-)-epicatechin-3-o-gallate (ECG), verified by UPLC, was 98%, 82% and 71%, respectively. The mean polymerization degrees of the three OPCs fractions were respectively 2.842, 3.521, 4.475. As compared with traditional separation methods for catechins and OPCs, HSCCC has shown to be a powerful and efficient technique for large-scale isolation of these compounds.

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Preparative purification of plasmin activity stimulating phenolic derivatives from Gastrodia elata using centrifugal partition chromatography

Jeon

Kim

Park

et al. 2015

Biomedical Chromatography

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Gastrodia rhizome, a dried and steamed tuber of Gastrodia elata Blume (Orchidaceae), has been traditionally used in Korea, China and Japan for the treatment of neurological and nervous disorders such as headaches, dizziness, vertigo and convulsive illnesses. The ethyl acetate and water extracts of G. elata stimulated plasmin activity. The active ethyl acetate fraction was subjected to centrifugal partition chromatography (CPC) with a two-phase solvent system, composed of n-hexane-ethyl acetate-methanol-water (3:7:4:6, v/v) followed by semi-preparative HPLC purification to separate active compounds and the water fraction was purified by Diaion HP-20 resin and semi-preparative HPLC. In ethyl acetate extract, 4-hydroxybenzyl alcohol (1), 4-hydroxybenzoic acid (2), 4-hydroxybenzaldehyde (3), 4-ethoxymethylphenol (4), 4,4'-oxybis(methylene)diphenol (5) and 4,4'-methylenediphenol (6) were obtained with high purities. Parishin (7) and parishin B (8) were isolated from water extract. Among isolated compounds, 4-hydroxybenzyl alcohol (1), 4-hydroxybenzaldehyde (3) and 4-ethoxymethylphenol (4) significantly stimulated plasmin activity. Copyright © 2015 John Wiley & Sons, Ltd.

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Preparative isolation and purification of theaflavins and catechins by high-speed countercurrent chromatography

Cited by 42 publications

References 24 publications

The hexane-ethyl acetate-methanol-water system for the separation of theaflavins from black tea (Camellia sinensis) using high-speed counter-current chromatography

The hexane-ethyl acetate-methanol-water system for the separation of theaflavins from black tea (Camellia sinensis) using high-speed counter-current chromatography

Preparative isolation of monomer catechins and oligomer procyanidin fractions from grape seed extracts by high-speed counter-current chromatography

Preparative purification of plasmin activity stimulating phenolic derivatives from Gastrodia elata using centrifugal partition chromatography

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