Preparative purification of rebaudioside A from aqueous extracts using chromatography: a process idea

Bergs, Dominik; Merz, J.; Delp, Axel; Joehnck, Matthias; Martin, Georg; Schembecker, Gerhard

doi:10.1007/s00003-012-0784-x

Cited by 14 publications

(6 citation statements)

References 11 publications

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“…These methods are not appropriate for industrial process due to several drawbacks including complicated and time-consuming operations, consumption of organic solvents and inevitably environmental contamination. Recently, Bergs et al [6] developed a simplified and efficient chromatographic process for separation of RA from aqueous extracts of Stevia rebaudiana leaves. However, the process required a cascade of expensive preparative chromatographic columns.…”

Section: Introductionmentioning

confidence: 99%

Adsorption characteristics of stevioside and rebaudioside A from aqueous solutions on 3-aminophenylboronic acid-modified poly(divinylbenzene-co-acrylic acid)

Yang

Xiao

et al. 2013

Separation and Purification Technology

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Section: Introductionmentioning

confidence: 99%

Adsorption characteristics of stevioside and rebaudioside A from aqueous solutions on 3-aminophenylboronic acid-modified poly(divinylbenzene-co-acrylic acid)

Yang

Xiao

et al. 2013

Separation and Purification Technology

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“…The chromatographic method effectively separates substances by multiple distributions according to the distribution coefficients of different components in the stationary and mobile phases (Bergs et al., 2012). It has the characteristics of high resolution and fast separation.…”

Section: Purification Techniques Of Rebaudioside Amentioning

confidence: 99%

“…Bergs et al. (2012) used a cascade of three preparative chromatographic columns to purify rebaudioside A from aqueous extracts of stevia leaves. Rebaudioside A with a purity of 97.1% ± 1.5% was achieved.…”

Section: Purification Techniques Of Rebaudioside Amentioning

confidence: 99%

Natural and low‐caloric rebaudioside A as a substitute for dietary sugars: A comprehensive review

Wang

Luo

Chen³

et al. 2022

Comp Rev Food Sci Food Safe

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For health and safety concerns, traditional high-calorie sweeteners and artificial sweeteners are gradually replaced in food industries by natural and low-calorie sweeteners. As a natural and high-quality sugar substitute, steviol glycosides (SvGls) are continually scrutinized regarding their safety and application. Recently, the cultivation of organic stevia has been increasing in many parts of Europe and Asia, and it is obvious that there is a vast market for sugar substitutes in the future. Rebaudioside A, the main component of SvGls, is gradually accepted by consumers due to its safe, zero calories, clear, and sweet taste with no significant undesirable characteristics. Hence, it can be used in various foods or dietary supplements as a sweetener. In addition, rebaudioside A has been demonstrated to have many physiological functions, such as antihypertension, anti-diabetes, and anticaries. But so far, there are few comprehensive reviews of rebaudioside A. In this review article, we discuss the physicochemical properties, metabolic process, safety, regulatory, health benefits, and biosynthetic pathway of rebaudioside A and summarize the modification methods and state-of-the-art production and purification techniques of rebaudioside A. Furthermore, the current problems hindering the future production and application of rebaudioside A are analyzed, and suggestions are provided.

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“…However, these works were at lab-scale, and the high cost could restrict the industrial usage of the columns. Other methods such as ethanol pretreatment (Formigoni et al, 2018), affinity chromatography (Bergs et al, 2012) and centrifugal partition chromatography (Hubert et al, 2015) were also explored.…”

Section: Introductionmentioning

confidence: 99%

Purification of stevia extract by chitosan precipitation and reversed‐phase chromatography

Zhang

Xiao

Liu

et al. 2021

Int J of Food Sci Tech

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To improve the shortness of the current purification technology of steviol glycosides (SG) extract, a twostep purification procedure consisting of chitosan precipitation and reversed-phase chromatography (RPC) was investigated. The impurity-to-SG ratio in the SG extract obtained from non-destructive soaking was 5:1~6:1. It was found that 80.90% of the impurities could be removed by RPC using 20% (v/v) ethanol solution as the eluent with a high SG recovery of 96.98%. When 25% ethanol was applied, the SG recovery was reduced to 71.23%. An adsorption test using D280 anionic resin suggested that the impurities were negatively charged; thus, chitosan was used to co-precipitate the impurities. An optimal decolourisation result of~70% was obtained at 0.06 gÁg −1 (w/w, to solids in extract) chitosan addition, shaking at 28°C for 1 h. Afterwards, the extract was further purified by RPC. The total removal rate of impurities was increased to 91.20%, and the SG recovery was 85.15%.

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Preparative purification of rebaudioside A from aqueous extracts using chromatography: a process idea

Cited by 14 publications

References 11 publications

Adsorption characteristics of stevioside and rebaudioside A from aqueous solutions on 3-aminophenylboronic acid-modified poly(divinylbenzene-co-acrylic acid)

Adsorption characteristics of stevioside and rebaudioside A from aqueous solutions on 3-aminophenylboronic acid-modified poly(divinylbenzene-co-acrylic acid)

Natural and low‐caloric rebaudioside A as a substitute for dietary sugars: A comprehensive review

Purification of stevia extract by chitosan precipitation and reversed‐phase chromatography

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