Preparative separation and purification of steroidal saponins inParis polyphyllavar.yunnanensisby macroporous adsorption resins

Abstract

Context: Saponins are active compounds in natural products. Many researchers have tried to find the method for knowing their concentration in herbs. Some methods, such as solid-liquid extraction and solvent extraction, have been developed. However, the extraction methods of the steroidal saponins from Paris polyphylla Smith var. yunnanensis (Liliaceae) are not fully researched. Objective: To establish a simple extraction method for the separation of steroidal saponins from the rhizomes of P. polyphylla Smith var. yunnanensis. Materials and methods: Macroporous adsorption resins were used for the separation of steroidal saponins. To select the most suitable resins, seven kinds of macroporous resins were selected in this study. The static adsorption and desorption tests on macroporous resins were determined. Also, we optimized the temperature and the ethanol concentration in the extraction method by the contents of five kinds of saponins. Then, we compared the extraction method with two other methods. Results: D101 resin demonstrated the best adsorption and desorption properties for steroidal saponins. Its adsorption data fits best to the Freundlich adsorption model. The contents of steroidal saponins in the product were 4.83-fold increased with recovery yields of 85.47%. Discussion and conclusion: The process achieved simple and effective enrichment and separation for steroidal saponins. The method provides a scientific basis for large-scale preparation of steroidal saponins from the Rhizoma Paridis and other plants.

Inhibition of lung cancer in diethylnitrosamine‐induced mice by Rhizoma paridis saponins

“…RPS was prepared as previously described. D101 resin was used for large‐scale enrichment and separation of steroidal saponins . High‐performance liquid chromatographic (HPLC) with evaporative light scattering detection (ELSD) and HPLC with electrospray ionization multistage tandem mass spectrometry (HPLC‐ESI‐MS( n )) were used to identify and quantify steroid saponins in RPS.…”

“…D101 resin was used for large-scale enrichment and separation of steroidal saponins. 14 High-performance liquid chromatographic (HPLC) with evaporative light scattering detection (ELSD) and HPLC with electrospray ionization multistage tandem mass spectrometry (HPLC-ESI-MS(n)) were used to identify 15 and quantify 16 steroid saponins in RPS. The content of the known saponins such as Paris I, II, III, V, VI, VII, H, and gracillin was determined simultaneously using the developed HPLC-ELSD method.…”

Antitumor and anti‐metastatic mechanisms of Rhizoma paridis saponins in Lewis mice

“…A voucher specimen (GWCL201009) was deposited at the School of Pharmaceutical Science and Technology at Tianjin University, Tianjin, China. RPS was prepared as previously described . In briefly, the dried crushed roots of Paris polyphylla were extracted with 70% ethanol.…”

Global metabolic profiling for the study of Rhizoma Paridis saponins‐induced hepatotoxicity in rats

“…The voucher specimen (GWCL201309) was deposited at the School of Pharmaceutical Science and Technology at Tianjin University, Tianjin, China. RPS was prepared and identified as previously described (Liu et al, ).…”