Preparative Separation and Purification of Rebaudioside A from Steviol Glycosides Using Mixed-Mode Macroporous Adsorption Resins

Abstract

Preparative separation and purification of rebaudioside A from steviol glycosides using mixed-mode macroporous adsorption resins (MARs) were systematically investigated. Mixed-mode MARs were prepared by a physical blending method. By evaluation of the adsorption/desorption ratio and adsorption/desorption capacity of mixed-mode MARs with different proportions toward RA and ST, the mixed-mode MAR 18 was chosen as the optimum strategy. On the basis of the static tests, it was found that the experimental data fitted best to the pseudosecond-order kinetics and Temkin-Pyzhev isotherm. Furthermore, the dynamic adsorption/desorption experiments were performed on the mini column packed with mixed-mode MAR 18. After one run treatment, the purity of rebaudioside A in purified product increased from 40.77 to 60.53%, with a yield rate of 38.73% (W/W), and that in residual product decreased from 40.77 to 36.17%, with a recovery yield of 57.61% (W/W). The total recovery yield reached 96.34% (W/W). The results showed that this method could be utilized in large-scale production of rebaudioside A from steviol glycosides in industry.

Evaluation on the adsorption capability of chemically modified macroporous adsorption resin with ionic liquid

“…The adsorption capacity was maintained at a dynamic equilibrium with increasing adsorption time. This result implied that the adsorption of ECG on the three resins could be classified as the slow adsorption type [27]. The adsorption capacity of ECG on ionic liquid/MAR was higher than on the other resins.…”

Study on Selective Removal of Impurity Iron from Leached Copper-Bearing Solution Using a Chelating Resin

“…Within the initial 6 min, a linear and rapid increase in iron removal by adsorption was observed, followed by a gradual increase until 30 min. The adsorption tended to be equivalent after 60 min, indicating a rapid adsorption resin [27]. It should be noted that the iron adsorption by resin varied insignificantly when temperature increased from 298 to 328 K within 6 min, but over 60% of iron was adsorbed within this period.…”

“…Minerals 2016, 6, 106 6 of 12 adsorption resin [27]. It should be noted that the iron adsorption by resin varied insignificantly when temperature increased from 298 to 328 K within 6 min, but over 60% of iron was adsorbed within this period.…”

“…It should be noted that the iron adsorption by resin varied insignificantly when temperature increased from 298 to 328 K within 6 min, but over 60% of iron was adsorbed within this period. In the following adsorption stage, the iron adsorption rate increased gradually with the increased temperature, although the final removal of iron (approximately 95%) was almost the same at 90 min, achieving a Q e ranging from 18.43 to 18.74 mg·g −1 from 298 to 328 K. adsorption resin [27]. It should be noted that the iron adsorption by resin varied insignificantly when temperature increased from 298 to 328 K within 6 min, but over 60% of iron was adsorbed within this period.…”

Simple extraction and membrane purification process in isolation of steviosides with improved organoleptic activity

“…The long isolation procedures of steviol glycosides known to produce noxious residues and biter alkaloids harmful to the human health [17,18]. Some of the recent stevioside isolation methodologies are expensive and are energy intensive process in obtaining pure steviol glycosides' [9][10][11]19]. Therefore, a simple, inexpensive and environmental friendly procedure in isolation of highly purified steviol glycosides is present day challenging problem [20][21][22][23].…”