Preparative separation of chlorogenic acid by centrifugal partition chromatography from highbush blueberry leaves (Vaccinium corymbosum L.)

Kim, Sang-Min; Shang, Ya Fang; Um, Byung-Hun

doi:10.1002/pca.1218

Cited by 35 publications

(18 citation statements)

References 19 publications

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“…In the case of CPC, this mechanism is based on hydrostatic force, formed by the centrifugal field in the rotor in one-axis centrifuge. CPC was applied by Kim et al [109] for preparative isolation of 5-CQA from highbush blueberry leaves (Vaccinium corymbosum L.). A water fraction containing a high concentration of 5-CQA (14.5 % of dry weight extract) was obtained by defatting a crude methanol extract from blueberry leaves.…”

Section: Chromatographic Analysismentioning

confidence: 99%

Chlorogenic acids – their properties, occurrence and analysis

Gil

Wianowska

2017

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Chlorogenic acids (CQAs), the esters of caffeic acid and quinic acid, are biologically important phenolic compounds present in many plant species. Nowadays much is known from their pro-health properties, including anti-cancer activity. Yet, the supposition that they may be helpful in fighting obesity and modify glucose-6-phosphatase involved in glucose metabolism have led to a revival of research on CQAs properties and their natural occurrence. Much attention is also paid to the proper analysis of CQAs content in plants and plant products due to the fact that the main CQAs representative in nature i.e. 5-O-caffeoylquinic acid (5-CQA) is commonly employed as a quality marker in the control of various natural products.The common procedures used so far for CQAs determination in plants involve conventional long lasting solvent extraction realized at higher temperatures prior to chromatographic analysis. The drawbacks associated to the conventional extraction techniques have led to the search for new alternative extraction processes that additionally could improve extracts quality. The latter is particularly important as regards CQAs applications, and the fact that these compounds easily transform/degrade to others. According to reports, the conventional heating of 5-CQA in the presence of water causes its isomerization and transformation. The reports prove that 5-CQA not only isomerizes to 3- and 4-O-caffeoylquinic acid, but also undergoes other transformations such as esterification and reactions with water. Hence, in the attempt to improve the process of chlorogenic acids extraction and to make it more effective and environmentally friendly, innovative so-called "enhanced" techniques of extraction have been recently developed and applied. To guarantee the proper analysis of compounds with very similar properties forming the chlorogenic acids family and their numerous transformation/degradation products, hyphenated techniques, in particular LC-MS, are currently being used.

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Section: Chromatographic Analysismentioning

confidence: 99%

Chlorogenic acids – their properties, occurrence and analysis

Gil

Wianowska

2017

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“…Blueberry ( V. corymbosum L.) leaves are the aerial parts of the plant, which have been characterized by their medicinal uses for centuries [ 51 ]. The leaves have varying phenolic compositions during different seasons; this fact is illustrated in the changing of their color from green to red in the autumn.…”

Section: Introductionmentioning

confidence: 99%

The Chemical and Biological Profiles of Leaves from Commercial Blueberry Varieties

Ștefănescu

Călinoiu

Ranga

et al. 2020

Plants

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Blueberries have seen an ascending production line boosted by World Health Organization (WHO) approvals for their contributions to a healthy diet and the evidence that they act against different diseases. This increase resulted in significant amounts of discarded leaves, which could be a valuable source of bioactive compounds. In the present study, ultrasound-assisted extraction technology was used to determine and compare the chemical and biological profiles of leaves from six commercial blueberry (Vaccinium corymbosum L.) varieties. Feruloylquinic acid was the major compound identified, ranging from 19.23 ± 0.18 mg/g (at the lowest level, registered in the Spartan variety) to 49.62 ± 0.41 mg/g (at the highest level, registered in the Nelson variety). Rutin was the second major compound identified, for which Toro, Nelson, and Elliot leaves registered the highest values, with 35.77 ± 0.19 mg/g, 32.50 ± 0.20 mg/g, and 31.53 ± 0.1 mg/g, respectively. Even though analogous polyphenols were detected in the six cultivars, their concentrations and amounts were different. The leaf extracts of the cultivars Toro, Elliot, and Nelson appear to be good sources of antioxidants, registering high percentage inhibitions of DPPH radicals, of 70.41%, 68.42%, and 58.69%, respectively. The blueberry leaf extracts had a strong antibacterial activity and a low antifungal capacity, and a low-to-moderate antimutagenic capacity towards Salmonella typhimurium TA98 and TA100 strains, with Toro leaf being the best candidate. All of these biological activities indicate health-related benefits, recommending them as suitable candidates for medical and pharmaceutical applications. The present paper adds significant knowledge to the field of blueberry leaves via chemical and biological profiles, supporting the ultrasound-assisted extraction technique as a useful and green method to provide alternative sources of bioactive compounds.

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“…Blueberry leaves have been considered as an important source of phenolic compounds [1,2], which can be processed using several postharvest applications, including extraction, separation, purification, and encapsulation leading to viable product development and commercialisation of different blueberry phenolic compounds (chlorogenic acid [3], catechin, epicatechin, caffeic acid, and different anthocyanins [4]) or for application in nutraceutical purposes and as food additives. Blueberry leaves have been reported to have antioxidant activities comparable or higher than the fruits [1,2,5] and several other health beneficial effects including antidiabetic [6] and antibacterial effects [7].…”

Section: Introductionmentioning

confidence: 99%

Effect of Postharvest LED Application on Phenolic and Antioxidant Components of Blueberry Leaves

2018

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Light from red (661 nm) and blue (417 nm) LEDs were applied for 12, 24, and 48 h on freshly harvested blueberry leaves of different cultivars mixed together. The extracts obtained through microwave extraction of these leaves were analysed in terms of total phenolic content, total monomeric anthocyanin content, and antioxidant activity as measured by % scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and ferric reducing antioxidant potential (FRAP). It was observed that although the content of total phenolic content was high in the untreated leaves, there was an increase in the phenolic content and monomeric anthocyanin content of the leaves treated with blue light. DPPH inhibition activity and FRAP for all the samples were high; however, there was an increase in the FRAP of samples treated with light for different durations, which varied with type of light and the time of application of the LED light.

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Preparative separation of chlorogenic acid by centrifugal partition chromatography from highbush blueberry leaves (Vaccinium corymbosum L.)

Abstract: 5-CQA was successfully isolated from blueberry leaves by the CPC method in a one-step procedure, indicating a further potential use for blueberry leaves.

Cited by 35 publications

References 19 publications

Chlorogenic acids – their properties, occurrence and analysis

Chlorogenic acids – their properties, occurrence and analysis

The Chemical and Biological Profiles of Leaves from Commercial Blueberry Varieties

Effect of Postharvest LED Application on Phenolic and Antioxidant Components of Blueberry Leaves

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