Preparing for implantation

Kim, Julie

doi:10.7554/elife.73739

Cited by 4 publications

(5 citation statements)

References 6 publications

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“…After implantation, the embryo damages the luminal epithelium, and the stromal cells surrounding the implanted embryo are decidualized, and this process is regulated by the hormone progesterone [ 7 , 8 ]. Decidualization includes the emerging of new types of endometrial cells, epithelioid cells, which provide nutrition and support for the implanting blastocyst until the placenta is fully formed [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Epigenetic and Molecular Factors in Endometrium of Females with Infertility

et al. 2022

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Infertility is one of the most rapidly increasing global health concerns of the 21st century. Embryo quality and endometrial thickness and receptivity are the main factors for successful embryo implantation and pregnancy development. Nevertheless, until now, there has been a lack of understanding about the regulation of human endometrium function and its structure. This raises the demand for more research of the human endometrium in these fields. In our study, we analyzed the genetic and epigenetic changes of endometrial tissue’s samples isolated from females admitted for treatment due to male infertility and females diagnosed with reproductive pathologies, who are preparing for assisted reproductive technologies procedures. Using real-time polymerase chain reaction method, we demonstrated that endometrium of females with reproductive pathology has significantly upregulated decidualization related genes HAND2, MUC1, CSF2, increased expression of angiogenesis related gene PDGFA, and increases of overall immune response and inflammation-related genes expression with significant changes of RELA and CXCL10 genes expression. Females with reproductive pathology have altered endometrium epigenetic regulation since expression of miRNAs—specifically, miRNA-34a, miRNA-223, and miRNA-125b—is lower in endometrium of females with reproductive pathology. Our findings suggest that the potential changes in genetic and epigenetic profile of endometrium from females with reproductive pathology could enrich the knowledge in the field of core biological knowledge and treatment of reproductive impairments.

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Section: Introductionmentioning

confidence: 99%

Characterization of Epigenetic and Molecular Factors in Endometrium of Females with Infertility

et al. 2022

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“…Persistent alterations in SCN clock period are evoked by single clock-resetting light pulses as well as by entrainment to different photoperiods (Kim and McMahon, 2021; Pittendrigh and Daan, 1976a), and entrainment to different photoperiods alters the acute response of the SCN to light pulses (Glickman et al, 2012; Pittendrigh et al, 1984; VanderLeest et al, 2009), suggesting the possibility of mechanistic overlap between these responses to circadian light input. Therefore, we sought to examine whether the expression of acutely light responsive SCN genes might also be altered in our photoperiod treatments.…”

Section: Resultsmentioning

confidence: 99%

“…We describe a remarkable convergent regulation of three SCN light response and MAPK/ERK modulating genes - Rasd1 , Dusp4 and Gem - which potentially underlie plasticity of the SCN light response, and in addition, our dataset reveals several potentially novel SCN photoperiod plasticity genes including Prokr2 , Cck neuropeptide signaling genes and the clock-associated gene Timeless . Future experiments can test the potential novel mechanistic roles of specific genes from this dataset in SCN photoperiod entrainment and plasticity using ex vivo optogenetic entrainment (Kim and McMahon, 2021) combined with SCN specific gene manipulation.…”

Section: Discussionmentioning

confidence: 99%

Gene expression plasticity of the mammalian brain circadian clock in response to photoperiod

Cox,

Gianonni-Guzmán,

Cartailler

et al. 2024

Preprint

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Seasonal daylength, or circadian photoperiod, is a pervasive environmental signal that profoundly influences physiology and behavior. In mammals, the central circadian clock resides in the suprachiasmatic nuclei (SCN) of the hypothalamus and synchronizes, or entrains, physiology and behavior to the prevailing light cycle from retinal input. The process of entrainment induces considerable plasticity in the SCN, but the molecular mechanisms underlying SCN plasticity are incompletely understood. Entrainment to different photoperiods persistently alters the phase, waveform, period, and resetting properties of the SCN and its driven rhythms. To elucidate novel molecular mechanisms of photoperiod plasticity, we performed RNAseq on whole SCN dissected from mice raised in Long (LD 16:8) and Short (LD 8:16) photoperiods. Using differential rhythms analysis, we showed that fewer rhythmic genes were detected in Long photoperiod and that there was an overall phase advance of gene expression rhythms of 4-6 hours. However, a few genes showed significant phase delays, including GTP binding protein overexpressed in skeletal muscle (Gem), an SCN light responsive gene and light response modulator. Using differential expression analysis, we found significant expression changes in the clock-associated gene timeless circadian clock 1 (Timeless) and abundant changes in expression levels of SCN neural signaling genes related to light responses, neuropeptides, GABA, ion channels, and serotonin. Particularly striking were differences across photoperiods in the expression of the SCN neuropeptide signaling genes, prokineticin receptor 2 (Prokr2) and cholecystokinin (Cck), as well as convergent regulation of the expression of three SCN light response genes, dual specificity phosphatase 4 (Dusp4) and RAS, dexamethasone-induced 1 (Rasd1), andGem. Transcriptional modulation ofDusp4andRasd1,and phase regulation ofGem,are compelling candidate molecular mechanisms for photoperiod-induced plasticity in the SCN light response. Similarly, transcriptional modulation ofProkr2andCckmay critically support SCN neural network reconfiguration during photoperiodic entrainment. Our findings identify the SCN light response and neuropeptide signaling gene sets as rich substrates for elucidating novel mechanisms of photoperiod plasticity.

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“…The experimental conditions, such as temperature and https://doi.org/10.11002/fsp.2024.31.2.218 drying time, were established through preliminary experiments (data not shown). However, to better align with the specific requirements of this experiment, the original protocol described in the Kim (2021) patent was partially modified for the FTG group. The prepared samples were sealed in groups of three and stored in a -20℃ freezer.…”

Section: Materials and Pretreatmentsmentioning

confidence: 99%

“…The CON and STG samples were dried for 36 h, whereas the FTG samples were dried for 24 h. The shorter drying time for the FTG samples likely resulted in a higher moisture content. This drying time was chosen based on the 24 h drying time recommended in a patent for the production of hot-air dried oriental melon slices pretreated using freeze-thawing (Kim, 2021). Interestingly, the STG samples showed similar moisture content to the CON samples despite the steam pretreatment.…”

Section: Moisture and Soluble Solids Content Of Dried Oriental Melon ...mentioning

confidence: 99%

Physicochemical and organoleptic properties of hot-air dried oriental melon slices prepared using different physical pretreatments

Kim,

Jung

et al. 2024

Food Sci. Preserv.

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The oriental melon poses challenges in terms of long-term storage and distribution. Addressing these issues requires an extension of the storage life through appropriate processing. In this study, pretreated hot-air dried oriental melon slices (steamed and freeze-thawed) were prepared, and their physicochemical and organoleptic properties were examined. The control group (CON) consisted of hot-air dried oriental melon slices without pretreatment, while the steamed group (STG) and freeze-thawed group (FTG) consisted of hot-air dried oriental melon slices pretreated using steaming and freeze-thawing methods, respectively. The moisture contents detected in the CON, STG, and FTG groups ranged from 15.96% to 27.30%, with variations of 7.53 to 8.40 °Brix for the soluble solid contents. The CON group showed the highest Hunter color value for lightness (L*) and the lowest value for redness (a*). The texture profile analysis revealed the highest hardness and chewiness in the order of STG > CON > FTG. Considerable differences were observed in springiness and adhesiveness in the FTG group compared to the other groups. The organoleptic evaluation showed that the CON group had the highest sensory scores for overall preference. These findings indicate that the properties of hot-air dried oriental melon slices were influenced by physical pretreatments and that manufacturing these slices without pretreatment is the most effective method in terms of processing simplicity and cost efficiency.

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Preparing for implantation

Abstract: A new laboratory model helps to understand the role of senescent cells in fostering a uterine environment that can support an embryo.

Cited by 4 publications

References 6 publications

Characterization of Epigenetic and Molecular Factors in Endometrium of Females with Infertility

Characterization of Epigenetic and Molecular Factors in Endometrium of Females with Infertility

Gene expression plasticity of the mammalian brain circadian clock in response to photoperiod

Physicochemical and organoleptic properties of hot-air dried oriental melon slices prepared using different physical pretreatments

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