Prepartum teat apex colonization with Staphylococcus chromogenes in dairy heifers is associated with low somatic cell count in early lactation

Vliegher, Sarne De; Laevens, Hans; Devriese, Luc; Opsomer, G.; Leroy, Jo; Barkema, Herman W.; Kruif, Aart de

doi:10.1016/s0378-1135(02)00363-2

Cited by 85 publications

(101 citation statements)

References 19 publications

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“…Staphylococcus chromogenes was the CoNS species most frequently isolated (74.07%) from dairy cows with IMI in this study. Other studies have also reported S. chromogenes as the most prevalent species isolated in milk samples from cows with mastitis caused by CoNS (3,(39)(40)(41)(42)(43), although with a lower frequency of isolation than observed in the present study.…”

Section: Discussioncontrasting

confidence: 58%

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2014

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bCoagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of the limitations related to the specific diagnosis of CoNS is the lack of an accurate, rapid, and convenient method that can differentiate the bacterial species comprising this group. The aim of this study was to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to accurately identify CoNS species in dairy cow IMI. In addition, the study aimed to determine the frequency of CoNS species causing bovine IMI. A total of 108 bacterial isolates were diagnosed as CoNS by microbiological cultures from two milk samples collected from 21 dairy herds; the first sample was collected at the cow level (i.e., 1,242 composite samples from all quarters), while the second sample was collected at the mammary quarter level (i.e., 1,140 mammary samples collected from 285 cows). After CoNS isolation was confirmed by microbiological culture for both samples, all CoNS isolates (n ‫؍‬ 108) were genotypically differentiated by PCR restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and subjected to the MALDI-TOF MS identification procedure. MALDI-TOF MS correctly identified 103 (95.4%) of the CoNS isolates identified by PCR-RFLP at the species level. Eleven CoNS species isolated from bovine IMI were identified by PCR-RFLP, and the most prevalent species was Staphylococcus chromogenes (n ‫؍‬ 80; 74.1%). In conclusion, MALDI-TOF MS may be a reliable alternative method for differentiating CoNS species causing bovine IMI.

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Section: Discussioncontrasting

confidence: 58%

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2014

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“…Despite variations between herds and countries, others CNS i.e. S. chromogenes, S. simulans and S. epidermidis, in general, appear to be the most frequently isolated CNS from mammary secretion samples worldwide (De Vliegher et al 2003, Taponen et al 2006. Identiϐication to species level would be important if it reϐlects differences in virulence characteristics or epidemiology, e.g.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance and detection of mecA and blaZ genes in coagulase-negative Staphylococcus isolated from bovine mastitis

et al. 2012

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Pesq. Vet. Bras. 32(8) The present study evaluated the pheno-and genotypical antimicrobial resistance proϐile of coagulase-negative Staphylococcus (CNS) species isolated from dairy cows milk, specially concerning to oxacillin. Of 100 CNS isolates, the S. xylosus was the prevalent species, followed by S. cohnii, S. hominis, S. capitis and S. haemolyticus. Only 6% were phenotypically susceptible to the antimicrobial agents tested in disk diffusion assay. Penicillin and ampicillin resistance rates were signiϐicantly higher than others antimicrobials. Four isolates were positive to mecA gene (4%), all represented by the S. xylosus species. The blaZ gene was detected in 16% of the isolates (16/100). It was noticed that all mecA + were also positive to this gene and the presence of both genes was correlated to phenotypic beta-lactamic resistance. We conclude that CNS species from bovine milk presented signiϐicantly distinct antimicrobial resistance proϐiles, evaluated by phenotypic and genotypic tests, which has implications for treatment and management decisions. Accepted for publication on March 10, 2012. 2 Curso de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária (IV), Universidade Federal Rural do Rio de Janeiro (UFRRJ), BR 465 Km 7, Seropédica, RJ 23890-000, Brazil.3 Laboratório de Bacteriologia Veterinária, Depto Microbiologia Veterinária, IV, UFRRJ, Seropédica, RJ. *Corresponding author: miliane@ufrrj.br percentual de resistência à penicilina e ampicilina foi signiϐicativamente maior que aos outros antimicrobianos. Quatro isolados foram positivos para o gene mecA (4%), sendo todos representados pela espécie S.xylosus. O gene blaZ foi detectado em 16% dos isolados (16/100), sendo todos mecA positivos e a presença de ambos os genes foi correlacionada com a resistência fenotípica aos beta-lactâmicos. Foi possível concluir que as espécies de ECN provenientes de leite bovino apresentaram distintos perϐis de resistência antimicrobiana, avaliados por testes fenotípicos e genotípi-cos, podendo diϐicultar a adoção de medidas de tratamento e manejo dos animais.

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“…A somatic cell count exceeding 200 000 in 1 ml of milk and positive bacteriological cultures of milk without general symptoms were considered to be indicators of subclinical mastitis. Macroscopic changes in milk and/or local changes within the mammary gland as well as general symptoms with increased somatic cell count > 200 000/ml milk indicated clinical mastitis (De Vliegher et al 2003, Gentilini et al 2002, Moon et al 2007.…”

Section: Methodsmentioning

confidence: 99%

Haemolytic and proteolytic activity of coagulase-negative staphylococci isolated from mastitis cows

Bochniarz¹,

Wawron²

2012

Polish Journal of Veterinary Sciences

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The aim of the present study was to assess the haemolytic and proteolytic activity of coagulase-negative staphylococci (CNS) isolated from cows with mastitis. The study was conducted on 100 CNS strains: S. xylosus (n=28), S.chromogenes (n=26), S.haemolyticus (n=25), S. sciuri (n=14), S. warneri (n=4), S.hominis (n=2), S.saprophyticus (n=1); 22 CNS were isolated from cows with clinical mastitis and 78 from those with subclinical mastitis. The CNS studied showed the ability to produce only α-haemolysin and belonged to one strain -S. haemolyticus (21.0% of isolated CNS strains). Haemolysin-positive CNS were responsible for both clinical and subclinical mastitis (22.7% and 20.5%, respectively). The ability to produce protease was found in 31.0% of CNS belonging to two strains: S. chromogenes and S. sciuri. Protease-positive CNS were the etiological factor of both clinical and subclinical mastitis (31.8% and 30.8%, respectively). All S. xylosus, S. warneri, S. hominis, and S. saprophyticus strains were found protease-negative and haemolysin-negative, irrespective of whether they caused clinical or subclinical mastitis in cows.

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Prepartum teat apex colonization with Staphylococcus chromogenes in dairy heifers is associated with low somatic cell count in early lactation

Cited by 85 publications

References 19 publications

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Antimicrobial resistance and detection of mecA and blaZ genes in coagulase-negative Staphylococcus isolated from bovine mastitis

Haemolytic and proteolytic activity of coagulase-negative staphylococci isolated from mastitis cows

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