Prepulse inhibition of the acoustic startle reflex vs. auditory brainstem response for hearing assessment

Longenecker, Ryan J.; Alghamdi, Faisal; Rosen, Merri J.; Galazyuk, Alexander V.

doi:10.1016/j.heares.2016.06.006

Cited by 32 publications

(33 citation statements)

References 76 publications

(133 reference statements)

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“…It depends on synchronous activation of ANFs by synaptic release of glutamate from IHCs at the onset of sound. The minimum sound level required to evoke an ABR at a given frequency relates closely to the threshold of perception defined by the behavioral audiogram in mice and human (Elberling and Don, 1987;Longenecker et al, 2016). ABR thresholds in unexposed Vglut3 WT and Vglut3 ϩ/Ϫ mice at 9 -11 weeks of age were not significantly different from each other at any of the 8 test frequencies (Fig.…”

Section: Cochlear Function In Vglut3 Mice Before and After 100 Db Expmentioning

confidence: 65%

Vesicular Glutamatergic Transmission in Noise-Induced Loss and Repair of Cochlear Ribbon Synapses

Kim¹,

Payne²,

Yang-Hood³

et al. 2019

J. Neurosci.

117

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Noise-induced excitotoxicity is thought to depend on glutamate. However, the excitotoxic mechanisms are unknown, and the necessity of glutamate for synapse loss or regeneration is unclear. Despite absence of glutamatergic transmission from cochlear inner hair cells in mice lacking the vesicular glutamate transporter-3 (Vglut3 KO), at 9-11 weeks, approximately half the number of synapses found in Vglut3 WT were maintained as postsynaptic AMPA receptors juxtaposed with presynaptic ribbons and voltage-gated calcium channels (Ca V 1.3). Synapses were larger in Vglut3 KO than Vglut3 WT. In Vglut3 WT and Vglut3 ϩ/Ϫ mice, 8-16 kHz octave-band noise exposure at 100 dB sound pressure level caused a threshold shift (ϳ40 dB) and a loss of synapses (Ͼ50%) at 24 h after exposure. Hearing threshold and synapse number partially recovered by 2 weeks after exposure as ribbons became larger, whereas recovery was significantly better in Vglut3 WT. Noise exposure at 94 dB sound pressure level caused auditory threshold shifts that fully recovered in 2 weeks, whereas suprathreshold hearing recovered faster in Vglut3 WT than Vglut3 ϩ/Ϫ. These results, from mice of both sexes, suggest that spontaneous repair of synapses after noise depends on the level of Vglut3 protein or the level of glutamate release during the recovery period. Noise-induced loss of presynaptic ribbons or postsynaptic AMPA receptors was not observed in Vglut3 KO , demonstrating its dependence on vesicular glutamate release. In Vglut3 WT and Vglut3 ϩ/Ϫ , noise exposure caused unpairing of presynaptic ribbons and presynaptic Ca V 1.3, but not in Vglut3 KO where Ca V 1.3 remained clustered with ribbons at presynaptic active zones. These results suggest that, without glutamate release, noise-induced presynaptic Ca 2ϩ influx was insufficient to disassemble the active zone. However, synapse volume increased by 2 weeks after exposure in Vglut3 KO , suggesting glutamate-independent mechanisms.

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Section: Cochlear Function In Vglut3 Mice Before and After 100 Db Expmentioning

confidence: 65%

Vesicular Glutamatergic Transmission in Noise-Induced Loss and Repair of Cochlear Ribbon Synapses

Kim¹,

Payne²,

Yang-Hood³

et al. 2019

J. Neurosci.

117

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“…ABR wave one amplitudes would better assess these changes (Kujawa and Liberman, 2009; Lin et al, 2011). In a mirror study with the same AOE parameters we found that wave one amplitudes were dramatically decreased, especially at suprathreshold levels, and more importantly, that these deficits corresponded to PPI audiometric deficits (Longenecker et al, 2016). Therefore, we expect that these animals also experience a decrease in ABR wave one amplitudes, signifying peripheral deafferentation (Kujawa and Liberman, 2015).…”

Section: Discussionmentioning

confidence: 75%

“…ABRs were collected to serve as a control for PPI hearing assessments following sound exposure (Figure 3), however these measures were compared extensively in our previous work (Longenecker et al, 2016). Thresholds of the T+ group nearly returned to baseline levels 3 months following exposure, with an average increase across frequencies of 6.11 dB SPL ( F (1, 30) = 478.47, p < 0.001).…”

Section: Resultsmentioning

confidence: 99%

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Variable Effects of Acoustic Trauma on Behavioral and Neural Correlates of Tinnitus In Individual Animals

Longenecker

Galazyuk

2016

Front. Behav. Neurosci.

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The etiology of tinnitus is known to be diverse in the human population. An appropriate animal model of tinnitus should incorporate this pathological diversity. Previous studies evaluating the effect of acoustic over exposure (AOE) have found that animals typically display increased spontaneous firing rates and bursting activity of auditory neurons, which often has been linked to behavioral evidence of tinnitus. However, only a subset of studies directly associated these neural correlates to individual animals. Furthermore, the vast majority of tinnitus studies were conducted on anesthetized animals. The goal of this study was to test for a possible relationship between tinnitus, hearing loss, hyperactivity and bursting activity in the auditory system of individual unanesthetized animals following AOE. Sixteen mice were unilaterally exposed to 116 dB SPL narrowband noise (centered at 12.5 kHz) for 1 h under ketamine/xylazine anesthesia. Gap-induced prepulse inhibition of the acoustic startle reflex (GPIAS) was used to assess behavioral evidence of tinnitus whereas hearing performance was evaluated by measurements of auditory brainstem response (ABR) thresholds and prepulse inhibition PPI audiometry. Following behavioral assessments, single neuron firing activity was recorded from the inferior colliculus (IC) of four awake animals and compared to recordings from four unexposed controls. We found that AOE increased spontaneous activity in all mice tested, independently of tinnitus behavior or severity of threshold shifts. Bursting activity did not increase in two animals identified as tinnitus positive (T+), but did so in a tinnitus negative (T−) animal with severe hearing loss (SHL). Hyperactivity does not appear to be a reliable biomarker of tinnitus. Our data suggest that multidisciplinary assessments on individual animals following AOE could offer a powerful experimental tool to investigate mechanisms of tinnitus.

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“…When assessed at recovery times of 2 and 5 min after exposure, their thresholds to single harmonic FM sweeps (FM1; downward sweeping from 50 to 25 kHz 7 ) increased by only 2 dB from those estimated at pre-exposure or in sham exposure conditions. In contrast, other terrestrial mammals can experience significant TTS (8–40 dB) lasting up to several hrs or even days after exposure to similarly-intense band-limited sounds within their audiometric ranges 1 , 8 – 11 . A criterion of a minimum threshold increase of 6 dB has been adopted to define a significant TTS 2 , 12 .…”

Section: Introductionmentioning

confidence: 98%

Big brown bats (Eptesicus fuscus) successfully navigate through clutter after exposure to intense band-limited sound

Simmons

Ertman

Hom

2018

Sci Rep

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Echolocating big brown bats fly, orient, forage, and roost in cluttered acoustic environments in which aggregate sound pressure levels can be as intense as 100 to 140 dB SPL, levels that would impair auditory perception in other terrestrial mammals. We showed previously that bats exposed to intense wide-band sound (116 dB SPL) can navigate successfully through dense acoustic clutter. Here, we extend these results by quantifying performance of bats navigating through a cluttered scene after exposure to intense band-limited sounds (bandwidths 5–25 kHz, 123 dB SPL). Behavioral performance was not significantly affected by prior sound exposure, with the exception of one bat after exposure to one sound. Even in this outlying case, performance recovered rapidly, by 10 min post-exposure. Temporal patterning of biosonar emissions during successful flights showed that bats maintained their individual strategies for navigating through the cluttered scene before and after exposures. In unsuccessful flights, interpulse intervals were skewed towards shorter values, suggesting a shift in strategy for solving the task rather than a hearing impairment. Results confirm previous findings that big brown bats are not as susceptible to noise-induced perceptual impairments as are other terrestrial mammals exposed to sounds of similar intensity and bandwidth.

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Prepulse inhibition of the acoustic startle reflex vs. auditory brainstem response for hearing assessment

Cited by 32 publications

References 76 publications

Vesicular Glutamatergic Transmission in Noise-Induced Loss and Repair of Cochlear Ribbon Synapses

Vesicular Glutamatergic Transmission in Noise-Induced Loss and Repair of Cochlear Ribbon Synapses

Variable Effects of Acoustic Trauma on Behavioral and Neural Correlates of Tinnitus In Individual Animals

Big brown bats (Eptesicus fuscus) successfully navigate through clutter after exposure to intense band-limited sound

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