Presença De Fungos Com Potencial Patogênico Em Instrumentos De Tosa

Mattei, Antonella Souza; Madrid, Isabel Martins; Santin, Rosema; Schuch, Luiz Filipe Damé; Meireles, Mário Carlos Araújo

doi:10.5380/avs.v19i2.32998

Cited by 3 publications

(8 citation statements)

References 22 publications

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“…In this study, only one dog positive for T. caninum infection presented dermatological abnormalities, characterized by multifocal alopecia, meliceric crusts, and generalized desquamation. Nevertheless, the present results should be interpreted cautiously, since this dog was also positive for dermatophytosis based on fungal culture (Mattei et al, 2014), which affects keratinized tissues (Nweze, 2011), and leads to similar morphological changes as described in the presented dog (Bond, 2010).…”

Section: Discussionsupporting

confidence: 71%

Clinical-dermatological, histological abnormalities and prevalence of Trypanosoma caninum and Leishmania infantum in dogs from Midwest region of Brazil

Makino

Moreira

Bezerra

et al. 2020

Rev. Bras. Parasitol. Vet.

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Leishmania infantum is a trypanosomatid that causes parasitic dermatopathy in dogs. Trypanosoma caninum is another trypanosomatid, which infects the skin of dogs, although cutaneous abnormalities are absent. This study aimed to investigate the occurrence of T. caninum infection and its associated cutaneous and histological changes and compare it with the occurrence of L. infantum infection in dogs. The study included 150 dogs, of which T. caninum infection was identified in 3 (2%) and L. infantum infection in 15 (10%) of them, with no association (p>0.05) of these infections with the breed, gender, age, or cutaneous abnormalities. The cutaneous abnormalities were based on 1 (4.8%) and 12 (57.1%) dogs infected by T. caninum and L. infantum, respectively. The dermatohistopathological abnormalities in the dogs infected with T. caninum included mild perivascular lymphohistioplasmacytic infiltrates in the clinically asymptomatic ones, while in those with dermatological abnormalities, acanthosis, epidermal orthokeratotic hyperkeratosis, melanomacrophages, and co-infection with Microsporum sp. and Trichophyton sp. were observed. InL. infantum infected, the histopathological findings included chronic granulomatous inflammatory infiltrates and structures compatible with amastigotes. Despite the low frequency of T. caninum infection, our findings suggest that this trypanosomatid, unlike L. infantum, does not cause any macroscopic skin abnormalities.

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Section: Discussionsupporting

confidence: 71%

Clinical-dermatological, histological abnormalities and prevalence of Trypanosoma caninum and Leishmania infantum in dogs from Midwest region of Brazil

Makino

Moreira

Bezerra

et al. 2020

Rev. Bras. Parasitol. Vet.

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“…(13.3%), Microsporum canis (13.3%), and Trichosporon (6.7%) (Figure 1). Studies show that dermatophytes, nondermatophyte filamentous fungi (NDFF) and yeasts with pathogenic potential have been isolated in clipping instruments and bath utensils in veterinary clinics and Pet Shops (Bagcigil et al, 2010;Mattei et al, 2011;Mattei et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…Studies show that dermatophytes, nondermatophyte filamentous fungi (NDFF) and yeasts with pathogenic potential have been isolated in clipping instruments and bath utensils in veterinary clinics and Pet Shops (Bagcigil et al, 2010;Mattei et al, 2011;Mattei et al, 2014). In the absence of data in Sinop -MT, this study aimed to isolate potentially pathogenic fungi in clipping instruments used in Pet Shops capable to cause mycotic zoonoses.…”

Section: Introductionmentioning

confidence: 99%

Mycotic zoonoses risk from clipping instruments in Pet Shops in Sinop – MT

et al. 2021

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Pets have conquered the daily lives of families worldwide and because they have a close relationship with humans, they can transmit mycotic zoonoses such as dermatophytosis, malassezioses and candidosis. Studies show that fungi with pathogenic potential have already been granted in clipping instruments and bath articles in veterinary clinics and in Pet Shops. In the absence of data in the city of Sinop - MT, this study aimed to isolate fungi with pathogenic potential in clipping instruments used in the routine of Pet Shops and to identify etiological agents capable of causing mycotic zoonoses. Samples were carried out in 18 clipping instruments, without cleaning, from 10 Pet Shops (A, B, C, D, E, F, G, H, I, J) in the city of Sinop - MT with swabs and sterile carpets square and then seeded in Sabouraud agar with 0.05% chloramphenicol and mycobiotic agar, incubated at room temperature (25°C) for 30 - 45 days. The dermatophytes and non-dermatophyte filamentous fungi were identified using the microculture technique and urease test and as yeasts using a urease test, zymogram, auxanogram, germ tube and corn meal agar with polysorbate 80. Of the samples from 18 clipping instruments, 15 were positive for at least one fungal genus. The yeast that showed a high prevalence of isolation in clipping instruments from the studied Pet Shops (B, C, D, E, H and J) was Malassezia pachydermatis (86.7%), followed by the genus Candida spp. (C and D; 26.7%), filamentous fungi of the genus Aspergillus spp. (A; 13.3%), Microsporum canis (B; 13.3%) and Trichosporon spp. (J, 6.7%). Our results demonstrate that Pet Shops treat a large number of animals daily in a single environment, which may amplify the risk of spreading zoonoses, as an asymptomatic or sick carrier animal can potentially transmit the microorganism to other animals inside the store and thus to a large number of new pet owners. There is a need to reinforce the commercial requirements specialized in bathing and grooming in the city of Sinop - MT on the good practices of cleaning and disinfection of the elements used and the environment, eliminating or eliminating the risk of contracting mycotic zoonoses.

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“…Dessa forma, deve-se destacar a importância da desinfecção, pois esta resulta na redução da carga microbiana (ANVISA, 2010;MATTEI et al, 2014).…”

Section: Introductionunclassified

“…Nas condições deste trabalho, comprovou-se a eficácia desse método de descontaminação em relação a C. albicans e C. gattii, assim como a necessidade de maiores pesquisas da atuação da UV diante do gênero Rhodotorula. (ANVISA, 2010;MATTEI et al, 2014).…”

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Luz Ultravioleta Na Inibição Do Crescimento De Leveduras

Silva¹,

Serra²,

Ripoll³

et al. 2017

SCI. ANIM. HEALTH

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RESUMO luz ultravioleta (UV) possui ação germicida, sendo amplamente utilizada na descontaminação de superfícies e materiais em laboratório. Essa radiação provoca alterações fotobioquímicas que promovem a inviabilidade ou morte dos microorganismos atingidos. As leveduras são células eucariotas, sendo assim, possuem núcleo que protege o seu material genético, necessitando de uma maior exposição à UV quando comparadas às células procariotas. O objetivo deste trabalho foi avaliar a eficiência da radiação ultravioleta sobre as leveduras expostas em cabine de segurança biológica. No estudo foram utilizados um isolado de Cryptoccocus gattii, um de Rhodotorula sp. e uma cepa padrão de Candida albicans (ATCC 14053). Utilizou-se a Norma M27-A2 do NCCLS para a preparação dos inóculos, diluindo-os nas concentrações 1, 5 e 10 da escala McFarland. Cada inóculo foi semeado em seis placas de Potato Dextrose Agar (PDA). Após, metade das placas foi exposta à radiação ultravioleta por 15 minutos em cabine de segurança biológica. Todas as placas foram incubadas em estufa a 37 °C por 10 dias. As placas semeadas com C. albicans e C. gattii e expostas à UV tiveram seu crescimento inibido. As placas semeadas com Rhodotorula sp. e expostas à radiação UV tiveram crescimento, porém com uma diminuição nas unidades formadoras de colônias (UFC) quando comparadas com as não expostas. Nas condições deste trabalho, comprovou-se a eficácia desse método de descontaminação em relação a C. albicans e C. gattii, assim como a necessidade de maiores pesquisas da atuação da UV diante do gênero Rhodotorula. Palavras-chave:Descontaminação. Desinfecção. Fungos. Fungicida. UV. INTRODUÇÃOFungos são seres ubíquos que se destacam por crescerem e se propagarem pela poeira ambiental, por isso, dificilmente um ambiente está livre da contaminação fúngica, fato que ocorre também em laboratórios e seus equipamentos (ANVISA, 2010;NOBRE;CLEFF, 2009).Dessa forma, deve-se destacar a importância da desinfecção, pois esta resulta na redução da carga microbiana (ANVISA, 2010;MATTEI et al., 2014).As instalações, equipamentos e procedimentos de um laboratório de micologia devem estar de acordo com as normas de biossegurança vigentes no país, visto que a correta aplicação desses regimentos contribui para a proteção das amostras processadas em relação a contaminantes externos, da equipe que manuseia e do meio ambiente (BRASIL, 2006; SANGIONI, 2012;UEKI et al., 2006).As cabines de segurança biológicas (CSB) são consideradas o principal equipamento de contenção de micro-organismos nos laboratórios, sendo capazes de proporcionar a

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Presença De Fungos Com Potencial Patogênico Em Instrumentos De Tosa

Cited by 3 publications

References 22 publications

Clinical-dermatological, histological abnormalities and prevalence of Trypanosoma caninum and Leishmania infantum in dogs from Midwest region of Brazil

Clinical-dermatological, histological abnormalities and prevalence of Trypanosoma caninum and Leishmania infantum in dogs from Midwest region of Brazil

Mycotic zoonoses risk from clipping instruments in Pet Shops in Sinop – MT

Luz Ultravioleta Na Inibição Do Crescimento De Leveduras

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