Presença De Mutações Em Cepas De Zika Virus Associadas a Microcefalia: Uma Análise in Silico

Messias, Thiago Silva; Silva, Kaique Cesar de Paula; Richini-Pereira, Virgínia Bodelão

doi:10.22533/at.ed.45019050719

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“…[19][20][21] The Japanese encephalitis virus (JEV), Saint Louis encephalitis virus (SLEV), and Tick-borne encephalitis virus (TBEV) presented the medium potential to cause CL/P. Using sequences of ZIKV strains associated with microcephaly, Paraíba (ZIKV-PA), Ceará (ZIKV-CE), and Belém (ZIKV-BE) 22 in the present method, we observed that ZIKV-PA and ZIKV-CE demonstrated similarity (E-value: 0.86) with patatin-like phospholipase domain containing 1 (PNPLA1), a gene that acts on lipid processing 23 and PR/ SET Domain 13 (PRDM13). They are active in the processes of cell state transition and promotion of several pathways connected to cellular DNA.…”

Section: Resultsmentioning

confidence: 99%

The Public Health Importance of Flaviviruses as an Etiological Environmental Factor in Nonsyndromic Cleft Lip and/or Palate: In silico Study

Silva

Messias

Soares

2022

The Cleft Palate Craniofacial Journal

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This in silico study aims to investigate flaviviruses as an environmental factor in the etiology of nonsyndromic cleft lip and/or palate (CL/P). A scoring method with 7 topics—disease, transplacental passage, tropism, cellular damage, reported case, analysis of genome similarity, and transcriptome between virus and host, was created based on literature and in silico experimentation. Viral genomes of NCBI virus were obtained and BLAST 2.12.0 was applied for the similarity analysis, adjusted to search for only human sequences related to CL/P with the statistical threshold defined for E-value ≤1. Flaviviruses with high potential to cause CL/P were: serotypes 2, 3, and 4 of the Dengue virus and lineage 2 of the West Nile virus, while the Yellow Fever virus, Japanese encephalitis virus, Tick-borne encephalitis virus, and Saint Louis encephalitis virus presented with medium potential to cause CL/P. As for the Zika virus, even strains associated with microcephaly showed only medium potential. Dengue virus and West Nile virus presented with high potential to act as environmental factors in the etiology of CL/P.

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