Presence and function of kisspeptin/KISS1R system in swine ovarian follicles

Basini, Giuseppina; Grasselli, Francesca; Bussolati, Simona; Ciccimarra, Roberta; Maranesi, Margherita; Bufalari, A; Parillo, Francesco; Zerani, Massimo

doi:10.1016/j.theriogenology.2018.04.006

Cited by 32 publications

(29 citation statements)

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“…testes (Hua et al, 2013;Hsu et al, 2014), in the ovaries of cats (Tanyapanyachon et al, 2018), dogs (Cielesh et al, 2017) and pigs (Basini et al, 2018) and in the testes of human (Feng et al, 2019) and frogs (Chianese et al, 2017). Moreover, in rhesus monkeys, positive immunostaining of GPR54 was noted in round spermatocytes, seminiferous tubules, Sertoli cells and spermatids (Tariq et al, 2013) and in the perimeter of the seminiferous epithelium (Irfan et al, 2016).…”

Section: Research Articlementioning

confidence: 99%

Immunocytochemical Detection of Kisspeptin Receptor and Its Association with Motility of Buffalo Bull (Bubalus bubalis) Spermatozoa

Hussain¹

2020

PVJ

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Kisspeptin is a powerful regulator of the hypothalamic-pituitary-gonadal axis. It acts through its receptor GPR54 to regulate sexual maturation, oogenesis, spermatogenesis and fertilization. The present research was implemented to evaluate the presence of kisspeptin receptor on various regions of buffalo bull spermatozoa and to decipher its relationship with different motility parameters of the fresh spermatozoa. Standard swim-up protocol was performed on fresh ejaculates from Nili-Ravi buffalo bulls to obtain three hypothetical layers, having spermatozoa with enhanced motility and normal morphology. The progressive sperm motility was measured in each layer by using phase contrast microscopy. Methanol fixed sperm smears from each layer were processed for standard immunocytochemistry procedure for the detection of kisspeptin receptor using specific antibodies. Maximum GPR54 immunoreactivity was observed in the upper regions (head, neck/midpiece) and moderate immunoreactivity was seen in the lower region (tail) of the spermatozoa in all the three layers. No significant difference in total GPR54 expression was observed in the spermatozoa from three layers and no relationship was observed between percentage motility and GPR54 like ir. Present findings suggest that the buffalo sperm motility is not influenced by GPR54 expression on different regions of the sperm. However, robust GPR54 expression in dorsal areas of buffalo sperm, would raise possibility of a covert function of kisspeptin in the bubaline sperm biology.

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Section: Research Articlementioning

confidence: 99%

Immunocytochemical Detection of Kisspeptin Receptor and Its Association with Motility of Buffalo Bull (Bubalus bubalis) Spermatozoa

Hussain¹

2020

PVJ

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“…In this regard, kisspeptin stimulated LH secretion from the primary cultures of ovine (Smith et al, 2008b) and porcine (Suzuki et al, 2008) anterior pituitary cells. In the porcine ovary, both kisspeptin and kisspeptin receptor are expressed in the granulosa cells and oocytes of developed follicles (Basini et al, 2018). Kisspeptin may function in the ovary for ovulation and development of the corpus luteum (Peng et al, 2013;Laoharatchatathanin et al, 2015).…”

Section: Kisspeptin Effects On Other Reproductive Organsmentioning

confidence: 99%

Review: kisspeptin and reproduction in the pig

Lents

2019

Animal

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The activation of the hypothalamic–pituitary axis is critical for the initiation and maintenance of reproductive cycles in pigs and is influenced by a number of factors, such as nutrition, metabolism and gonadal steroids. Kisspeptin is a neuropeptide that is expressed in discrete regions of the porcine hypothalamus and is positioned to mediate the action of many of these factors. The expression of kisspeptin in the pig hypothalamus does not appear to be regulated by gonadal steroids in the same way as other species. It is unclear if kisspeptin is mediating nutritional or metabolic effects on gonadotropin secretion in pigs as it takes large deficits in feed intake or BW to affect hypothalamic expression of the KISS1 gene in the porcine hypothalamus. There appears to be little genetic diversity in kisspeptin or its receptor that is useful for improving reproduction in swine. Both peripheral and central injection of kisspeptin strongly stimulates the secretion of gonadotropin hormones, LH and FSH, in gilts. Similarly, synthetic analogues have been developed and showed potential promise as tools to manage reproductive cycles in gilts and sows. Review of the literature nonetheless reveals that research on kisspeptin and its function in controlling reproduction in pigs has lagged that of other livestock species.

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“…In mammals, the Kisspeptin encoded by Kiss-1 metastasis-suppressor ( KISS1 ) gene regulates the release of gonadotropin releasing hormone at the hypothalamic level [ 1 , 2 ] and is widely known for its essential role in controlling of the initiating of puberty [ 3 , 4 ]. Recently, the Kisspeptin has been reported to show the local roles in the ovaries of humans [ 5 ], pigs [ 6 , 7 ], rats [ 8 ], hamsters [ 9 ], dogs [ 10 ], and cats [ 11 ]. It is observed that the KISS1 −/− mice fail to undergo pubertal transition and show the absence of mature follicles [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

STAT4 targets KISS1 to promote the apoptosis of ovarian granulosa cells

et al. 2020

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Background In mammals, it is known that the estradiol-17β (E2) is mainly synthetized in ovarian granulosa cells (GCs), and the excessive apoptosis of GCs induces the follicular atresia. Many studies have implicated the essential role of KISS1, with the pro-synthetic effect of E2 and the anti-apoptotic effect on GCs, in the mammalian folliculogenesis, and several STAT4 potential binding sites were previously predicted on the promoter of KISS1 in pigs. However, the biological effects of STAT4 on GCs and the molecular regulation between STAT4 and KISS1 remained largely unknown. Methods Using the porcine GCs as the cellular model, the overexpression plasmid, small interfering RNA, 5′-deletion and luciferase assay were applied to investigate the molecular mechanisms for STAT4 regulating the expression of KISS1. Results In this study, the STAT4 negatively regulated the mRNA and protein levels of KISS1 in porcine GCs, and the mRNA level of STAT4 was observed to significantly decrease from immature to mature follicles, which was inversed with that of KISS1. The relative luciferase activity of KISS1 promoter was significantly increased with deletion of the fourth potential binding site (− 305/− 295), and ChIP further confirmed that the STAT4 bound at − 305/− 295 region of KISS1. Besides, the STAT4 significantly regulated the mRNA levels of PDK1, FOXO3 and TSC2 of PI3K signaling pathway to promote the cell apoptosis and the percentage of cells at G0/G1 phase of cell cycle in GCs. Alternatively, the STAT4 significantly decreased the mRNA levels of CYP17, 3B-HSD, 17B-33 HSD, ESR1, and ESR2, as well as the concentration of E2 in GCs. Furthermore, interfering with the expression of STAT4 was observed to significantly stimulate the pro-synthetic effect of E2 and anti-apoptotic effect of KISS1 in GCs. Conclusions Collectively, the STAT4 might directly target at − 305/− 295 region of KISS1 to negatively regulate the transcription of KISS1, promote the cell apoptosis via PI3K signaling pathway, suppress the synthesis of E2 through the estrogen signaling pathway in porcine GCs. These proposed works could provide useful insight in further investigations on the molecular functionalities of STAT4 and KISS1 in the folliculogenesis of mammals.

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Presence and function of kisspeptin/KISS1R system in swine ovarian follicles

Cited by 32 publications

References 43 publications

Immunocytochemical Detection of Kisspeptin Receptor and Its Association with Motility of Buffalo Bull (Bubalus bubalis) Spermatozoa

Immunocytochemical Detection of Kisspeptin Receptor and Its Association with Motility of Buffalo Bull (Bubalus bubalis) Spermatozoa

Review: kisspeptin and reproduction in the pig

STAT4 targets KISS1 to promote the apoptosis of ovarian granulosa cells

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