Presence and Localization of a 30-kDa Basic Fibroblast Growth Factor-Like Protein in Rodent Testes*

Mayerhofer, Artur; Russell, Lonnie D.; Grothe, Claudia; Rudolf, Margit; Gratzl, Manfred

doi:10.1210/endo-129-2-921

Cited by 58 publications

(38 citation statements)

References 23 publications

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“…No expression of FGF-4 mRNA or protein was detected in adult Leydig cells. In the epithelium, previous studies have localised FGF-2 to pachytene spermatocytes (Mayerhofer et al 1991, Han et al 1993, Koike & Noumura 1994; our studies localised FGF-4 to pachytene spermatocytes and suggest autocrine actions on these cells through the FGFRs (Cancilla & Risbridger 1998). …”

Section: Discussionsupporting

confidence: 73%

“…The expression and localisation of FGFs-2 (Han et al 1993, Ueno et al 1987, Mayerhofer et al 1991, Mullaney & Skinner 1992, Koike & Noumura 1994 and -8 (Schmitt et al 1996, Valve et al 1997, Wu et al 1997 have been reported in testes, and the functions of FGFs-1 and -2 have been examined using in vitro cell systems. However, data are limited to FGF-2 regulation of gonocyte proliferation (Van Dissel-Emiliani et al 1996), and FGFs-1 and -2 regulation of Leydig cell function in vitro (Fauser et al 1988, Murono et al 1993, Laslett et al 1997.…”

Section: Introductionmentioning

confidence: 99%

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Discrete cell- and stage-specific localisation of fibroblast growth factors and receptor expression during testis development

Cancilla¹,

Davies²,

Ford-Perriss³

et al. 2000

Journal of Endocrinology

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Fibroblast growth factors (FGFs) are a family of heparin binding proteins involved in many biological processes. These growth factors act through tyrosine kinase receptors (FGFRs); we have previously used immunohistochemistry to study FGFRs-1-4 in foetal, immature and adult rat testes, and found a discrete cell-and stage-specific localisation. Alternative mRNA splicing of FGFRs-1-3 leads to functional variants (IIIb and IIIc) with distinct ligand binding affinities, therefore we have identified the specific expression of functional FGFR variants and the expression and localisation of FGF ligands in testes from foetal, immature and adult rats. Using reverse transcriptasepolymerase chain reaction (RT-PCR), we found that mRNAs for FGFR-1 IIIb and IIIc, FGFR-2 IIIc, FGFR-3 IIIc and FGFR-4 were expressed in foetal, immature and adult testes. Ligands FGFs-1-5, and -8, which can signal through these receptors, were also expressed in testes at each age. Localisation of the ligands FGFs-1, -3 and -4 to rat testes by immunohistochemistry showed a discrete cell-and stage-specific localisation that altered during testis development. This study has shown that the ligands FGFs-1, -3 and -4 are expressed in the testis and have the capacity to signal through appropriate receptors that are also co-localised or expressed in adjacent cell types in the testis. Collectively, the expression profiles of the seven FGFR variants and FGFs-1-5 and -8 suggest a functional importance in testicular development and spermatogenesis. It is concluded that, future studies on the role of other FGF ligands, in particular FGFs-1-4, are warranted.

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Section: Discussionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Discrete cell- and stage-specific localisation of fibroblast growth factors and receptor expression during testis development

Cancilla¹,

Davies²,

Ford-Perriss³

et al. 2000

Journal of Endocrinology

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“…bFGF exists in the testis (36,37), and its role in the function of nonspermatogenic Leydig and Sertoli cells has been suggested (38). Moreover, bFGF has been discovered recently in the nuclei of mouse spermatogenic cells (13).…”

Section: Resultsmentioning

confidence: 99%

Direct effect of basic fibroblast growth factor on gene transcription in a cell-free system.

Nakanishi

Kihara

Mizuno

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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Recent findings on the translocation of intact fibroblast growth factor (FGF) into the cell nucleus suggest that it functions directly in nuclear events. We examined the effect of human basic FGF (bFGF) on gene transcription in a cell-free system. When mouse genes encoding phosphoglycerate kinases 1 and 2 (Pgk-1 and Pgk-2) were transcribed by using nuclear extracts of Ehrlich ascites tumor cells, FGF affected transcription in different ways: in the presence of bFGF, transcription of the Pgk-1 gene was inhibited, whereas that of the Pgk-2 gene was enhanced. When viral genes were tested, transcription of the adenovirus major late DNA was slightly stimulated but that of the adenovirus early EIA DNA or the human immunodeficiency virus DNA was not changed by the addition of bFGF. Moreover, the presence of a distinct 5' upstream region of the Pgk-2 gene, which includes a negative cis-acting element, was required for transcription stimulation by bFGF. These results suggest that bFGF can regulate transcription directly in the nucleus in a gene-specific manner.

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“…The testicular distribution of NF-200, TH, NPY, synaptosomal associated protein of 25 kDa (SNAP-25) and mast cells tryptase was examined in monkey testes using an ABC-immunohistochemical method as described previously [31, 34, 35]. Specific antibodies were employed (mouse monoclonal antibody anti-NF-200: Boehringer Mannheim Inc., Indianapolis, Ind., USA; dilution 1:50; rabbit polyclonal antibody anti-TH: Chemicon International Inc., Temecula, Calif., USA, dilution 1:400; rabbit polyclonal antibody anti-NPY: Peninsula Laboratories Inc., Belmont, Calif., USA, dilution 1:70,000; mouse monoclonal antibody directed against a synaptosomal associated protein of 25 kDa, SNAP-25: Sternberger Monoclonals Inc., Baltimore, Md., USA, dilution 1:500; mouse monoclonal antibody antitryptase: Dako, Hamburg, Germany, dilution 1:50).…”

Section: Methodsmentioning

confidence: 99%

Neuronal Elements in the Testis of the Rhesus Monkey: Ontogeny, Characterization and Relationship to Testicular Cells

et al. 2000

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Intrinsic neuron-like cells expressing the catecholamine-biosynthetic enzyme tyrosine hydroxylase (TH) were recently identified in the testis of the prepubertal rhesus monkey. In this study, we characterized the neuron-like nature of these cells and examined distribution and frequency of neuronal elements in the testes of monkeys during postnatal development, puberty and adulthood. Using immunohistochemical methods, we detected both nerve fibers and cell bodies, immunoreactive for the neuronal markers neurofilament 200 (NF-200) and synaptosomal associated protein of 25 kDa (SNAP-25), TH and neuropeptide Y (NPY) in perivascular locations, intermingled with interstitial cells and close to the wall of seminiferous tubules. Marked age-related differences in the numbers of these neuronal elements became apparent, when we quantified NF-200-immunoreactive neuronal elements. Thus, intrinsic neuron-like cell bodies were found only in the testes from immature animals (i.e., until about 3 years of age). Conversely, nerve fibers, presumably representing mainly the extrinsic innervation, were observed at all ages although they became more prominent after the pubertal increase in LH and testosterone levels. Interestingly, another testicular cell type known to contain potent regulatory substances, mast cells, was found to be in close anatomical proximity to nerve fibers. The number of these cells, positively identified with an antibody to tryptase, increased significantly after puberty following the same pattern as nerve fibers. These results confirm that the testicular nervous system of the monkey is composed of two components, intrinsic nerve cells and extrinsic fibers, both of which are catecholaminergic and peptidergic in nature. Furthermore, both components show a marked degree of plasticity during development, especially around the time of puberty. The intratesticular locations of neuron-like cells and fibers suggest that catecholamines and neuropeptides are likely to have multiple sites of actions, and may affect Leydig cells, cells of the tubular wall and vascular cells directly and/or indirectly via intermediation of mast cells.

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Presence and Localization of a 30-kDa Basic Fibroblast Growth Factor-Like Protein in Rodent Testes*

Cited by 58 publications

References 23 publications

Discrete cell- and stage-specific localisation of fibroblast growth factors and receptor expression during testis development

Discrete cell- and stage-specific localisation of fibroblast growth factors and receptor expression during testis development

Direct effect of basic fibroblast growth factor on gene transcription in a cell-free system.

Neuronal Elements in the Testis of the Rhesus Monkey: Ontogeny, Characterization and Relationship to Testicular Cells

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