Presence and secretion of atrial natriuretic peptide from cultured human aortic endothelial cells

Brandt, Roland R.; Heublein, D. M.; Mattingly, M. T.; Pittelkow, Mark R.; Burnett, John C.

doi:10.1152/ajpheart.1995.268.2.h921

Cited by 8 publications

(5 citation statements)

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“…ET-1 secretion is regulated predominantly by transcription and is modulated by various hormones and physical forces (11,13). Only small amounts of ET-1 are stored in vascular endothelial cells and most are released after synthesis (14,15). These studies exclude acid-induced decreased ET-1 degradation, increased release of preformed ET-1 from cellular compartments, and accelerated conversion of big ET-1 to ET-1 as mechanisms of increased ET-1 secretion caused by reduced extracellular pH.…”

Section: Discussionmentioning

confidence: 99%

Reduced extracellular pH increases endothelin-1 secretion by human renal microvascular endothelial cells.

Wesson¹,

Simoni²,

Green³

1998

J. Clin. Invest.

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Because dietary acid increases renal secretion of endothelin-1 (ET-1) which is synthesized by renal microvascular endothelium, we examined if reduced extracellular pH increases ET-1 secretion by cultured human renal microvascular endothelial cells (RMVECs). Confluent cells were exposed to serum-free media for 24 h, then incubated in either control, acid, or alkaline serum-free media for 12 h. Standard growth media pH was 7.2 after equilibration with 5% CO2 at 37 degrees C and was made the pH of control media. Acid and alkaline media pH were 7.0 and 7.4, respectively. Added Hepes and Tris maintained all assigned pHs. Media ET-1 measured by RIA after column extraction was higher for RMVECs exposed to acid compared with control media (170.0+/-17.1 vs. 64.6+/-9.6 pM, P < 0.004) but those exposed to alkaline media (56.6+/-25.1 pM, P = NS vs. control) were not. Human aortic endothelial cells exposed to control, acid, and alkaline media had similar ET-1 (166.6+/-18.1, 139.3+/-18.5, and 205.9+/-25.3 pM, P = NS). The data show acid-stimulated ET-1 secretion by RMVECs but not aortic endothelial cells, demonstrating a new environmental factor that influences ET-1 secretion by renal microvascular endothelium and thereby possibly modulates endothelin-dependent processes in vivo.

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Section: Discussionmentioning

confidence: 99%

Reduced extracellular pH increases endothelin-1 secretion by human renal microvascular endothelial cells.

Wesson¹,

Simoni²,

Green³

1998

J. Clin. Invest.

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“…Previous evidence indicates that ANP can be expressed and secreted by aortic endothelial cells [100], suggesting a local action of ANP also in the endothelium. Mice with endothelial-specific deletion of NPR-A gene (NPR-A-ecKO) undergoing limb ischemia displayed impaired angiogenesis until 5 weeks post-surgery.…”

Section: Anp and Vascular Pleiotropic Effectsmentioning

confidence: 99%

Cardiovascular Pleiotropic Effects of Natriuretic Peptides

Forte

Madonna

Schiavon

et al. 2019

IJMS

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Atrial natriuretic peptide (ANP) is a cardiac hormone belonging to the family of natriuretic peptides (NPs). ANP exerts diuretic, natriuretic, and vasodilatory effects that contribute to maintain water–salt balance and regulate blood pressure. Besides these systemic properties, ANP displays important pleiotropic effects in the heart and in the vascular system that are independent of blood pressure regulation. These functions occur through autocrine and paracrine mechanisms. Previous works examining the cardiac phenotype of loss-of-function mouse models of ANP signaling showed that both mice with gene deletion of ANP or its receptor natriuretic peptide receptor A (NPR-A) developed cardiac hypertrophy and dysfunction in response to pressure overload and chronic ischemic remodeling. Conversely, ANP administration has been shown to improve cardiac function in response to remodeling and reduces ischemia-reperfusion (I/R) injury. ANP also acts as a pro-angiogenetic, anti-inflammatory, and anti-atherosclerotic factor in the vascular system. Pleiotropic effects regarding brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) were also reported. In this review, we discuss the current evidence underlying the pleiotropic effects of NPs, underlying their importance in cardiovascular homeostasis.

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“…ANP infusion also influences the sympathetic nervous system, probably by inhibiting the baroreceptormediated activation of renal sympathetic nerves as well as increasing noradrenaline release [405,414,415]. ANP causes antimitogenic and antiproliferative effects in glomerular mesangial cells, in vascular smooth muscle cells, and in the heart [416] and arterial wall [417]. ANP inhibits the synthesis of the potent vasoconstrictor endothelin-I [418] and induces apoptosis in neonatal rat cardiomyocytes [419].…”

Section: Biological Actions and Receptor Pharmacologymentioning

confidence: 99%

Endogenous Vasoactive Peptides

Webb

Ksander²

2010

Burger's Medicinal Chemistry and Drug Discovery

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The control of vasomotor tone regulates the overall cardiovascular system and its responses to physiological and pathophysiological stress. It provides differential blood flow to regional vascular beds under normal physiologic conditions, at times of normal stress, such as exercise, and during conditions of pathological stress, such as congestive heart failure. Although the vascular system regulates many of its functions at the site of the blood vessel itself, other factors that may control vascular tone include the autonomic nervous system, circulating hormones functioning in an endocrine role, and reflex metabolic control. Many newly discovered peptides were found to be widespread in autonomic and sensory neurons innervating the vasculature, and they possess potent vasoactive effects on many blood vessels. Vasoactive peptides have been shown to play an important role in many pathological situations, for example, asthma, arthritis, vascular headaches, hypertension, and other cardiovascular diseases. In this chapter, we will outline the evidence for the different actions of various vasoactive peptides, their interaction with other systems, the possible role they have in diseases, and the present and future therapeutic use of vasoactive peptide agonists and antagonists.

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Presence and secretion of atrial natriuretic peptide from cultured human aortic endothelial cells

Cited by 8 publications

References 0 publications

Reduced extracellular pH increases endothelin-1 secretion by human renal microvascular endothelial cells.

Reduced extracellular pH increases endothelin-1 secretion by human renal microvascular endothelial cells.

Cardiovascular Pleiotropic Effects of Natriuretic Peptides

Endogenous Vasoactive Peptides

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