Presence and species identity of rumen flukes in cattle and sheep in the Netherlands

Abstract

The purpose of the study was to gain knowledge about the prevalence and identity of rumen flukes (RF) in cattle and sheep in the Netherlands. Routine faecal examinations of diagnostic submissions between May 2009 and September 2014 showed a mean annual herd or flock RF prevalence of 15.8% for cattle and 8.0% for sheep. Prevalence in cattle was higher after 2012 than before, which may reflect a change in detection method as well as an increase in true prevalence. During November and December 2014, an abattoir survey was conducted to allow for scoring of rumen fluke burden and to obtain specimens for molecular species characterization. Over 8 visits to 5 abattoirs in areas deemed to pose a high risk for trematode infection, 116 cows and 41 sheep from 27 herds and 10 flocks were examined. Prevalence of RF was higher in beef cattle than in dairy cattle and higher in cattle than in sheep. Median fluke burden was >100 specimens per animal for most positive animals. Using a semi-quantitative RF density score as a gold standard, sensitivity and specificity of a modified quantitative Dorsman egg counting method were estimated at 82.6% and 83.3%, respectively. Of 14 collected adult rumen flukes, twelve (8 bovine and 4 ovine specimens) were identified as Calicophoron daubneyi. The other two, of bovine origin, were identified as Paramphistomum leydeni, which was unexpected as in other European countries all recently collected rumen flukes in both cattle and sheep were identified as C. daubneyi. The findings implicate that multiple rumen fluke species, intermediate host species and transmission cycles may play a role in rumen fluke infections in the Netherlands.

Paramphistomosis of Ruminants: An Emerging Parasitic Disease in Europe

“…A study in Ireland [18] confirmed a high prevalence of C. daubneyi in Irish sheep flocks, but also identified infection with another rumen fluke species, Paramphistomum leydeni, in a single animal. One case of P. leydeni infection in cattle has also been reported in the Netherlands [49]. This suggests that occasional infection from ruminant wildlife reservoir hosts, where P. leydeni has been identified previously [52], may also occur in livestock.…”

“…Since Gordon et al [20] proposed C. daubneyi as the primary rumen fluke species infecting livestock in Britain in 2013, a number of studies have been conducted in the UK and Ireland, all of which have confirmed this finding using molecular approaches to species identification [46][47][48]. Studies across Western Europe, in the Netherlands [49], Belgium [50], Italy [51] and Spain [17] have also confirmed, using molecular tools, the predominant rumen fluke species identified in livestock to be C. daubneyi. A study in Ireland [18] confirmed a high prevalence of C. daubneyi in Irish sheep flocks, but also identified infection with another rumen fluke species, Paramphistomum leydeni, in a single animal.…”

Paramphistomidose – eine Übersicht zu Epidemiologie und klinischer Symptomatik

“…Möglich ist dies, da die vom Großen Leberegel Fasciola hepatica bekannte Zwischenwirtschnecke Galba truncatula im gemäßigten Klima Europas mit entsprechender Feuchtigkeit weit verbreitet ist [27] [45]. Studien aus den vergangenen 20 Jahren zeigen vermehrte Nachweise von Pansenegeln, teilweise wurden sprunghafte Prävalenzanstiege verzeichnet: 1999 in Frankreich [38], 2009 und 2012 in Irland [72] [73] sowie 2013 in den Niederlanden [51]. In Irland erhöhte sich 2012 sogar die Mortalität bei Rindern infolge Paramphistomidose [72].…”

“…Die abrupten Prävalenzanstiege sind außerdem deshalb kritisch zu sehen, weil aufgrund der Verwechslungsgefahr zwischen Pansenegeleiern und Eiern des Großen Leberegels ausreichende Erfahrung mit der Methodik Voraussetzung für korrekte Ergebnisse ist [56]. In den Niederlanden wurde sogar Ende 2012 eine neue, hochsensitive koproskopische Methode eingeführt, was wahrscheinlich zu einer verbesserten Erkennung von Pansenegeleiern geführt hat, sodass sich höhere Prävalenzen ergaben als vorher [51].…”

Trematodoses in Cattle and Buffalo Around Schistosomiasis Endemic Areas in Central Sulawesi Province of Indonesia

“…Rerata geometrik telur Paramphistoma tiap gram tinja juga berbeda secara signifikan pada desa yang berbeda (P<0,05) (Tabel 8). Prevalensi Paramphistomiasis pada daerah lain di Indonesia dan di negara lainnya bervariasi antara 259% (Satyawardhana 2017;Melaku & Addis 2012;González-Warleta et al 2013;Szmidt-Adjidé et al 2000;Jones et al 2017;Ploeger et al 2017;Eslami et al 2011). Variasi prevalensi ini dapat terjadi karena perbedaan metode pemeliharaan, ukuran contoh, komposisi ternak, potensi biologi keong dan inang, serta iklim dan topografi lokasi pengambilan contoh (Chaudhary et al 2014).…”