Presence and type of oncogenic human papillomavirus in classic and in differentiated vulvar intraepithelial neoplasia and keratinizing vulvar squamous cell carcinoma

Abstract

The presence and type of oncogenic papillomavirus (HPV) in classic warty/basaloid vulvar intraepithelial neoplasia and in differentiated vulvar intraepithelial neoplasia and keratinizing vulvar squamous cell carcinoma was investigated using three techniques, that is, histology, in situ hybridization, and PCR-ELISA. HPV typing was performed using in situ hybridization and PCR-ELISA. Differentiated vulvar intraepithelial neoplasia and invasive keratinizing vulvar squamous cell carcinoma proved completely negative for HPV by PCR-ELISA, in situ hybridization, and histologic examination, while in classic vulvar intraepithelial neoplasia, a HPV positivity of 66.1% was found. HPV 16 was the predominant type, with HPV 35, 33, and 52 types found rarely and sometimes together with HPV 16. PCR-ELISA proved to be the most suitable method to detect and type mucosal oncogenic HPVs. The absolute absence of HPV DNA in differentiated vulvar intraepithelial neoplasias and in keratinizing vulvar squamous cell carcinoma suggests a strong HPV-independent pathway of malignant progression to invasive carcinoma.

Human papillomavirus type distribution in vulval intraepithelial neoplasia determined using PapilloCheck DNA Microarray

“…This is consistent with HPV associated disease being more common in younger women and vulval intraepithelial neoplasia associated with lichen sclerosis being more common in older patients [Bonvicini et al, 2005].…”

Examining the association between socioeconomic status and potential human papillomavirus-associated cancers

“…22 Evidence has established that HPV is a necessary cause for the development of many cancers, with other cofactors involved. [26][27][28][29][30][31][32][33][34][35] The present study extended previous research by examining the relation between SES and the incidence of potential HPVassociated cancers, namely vulvar, vaginal, anal, penile, and oral cavity and oropharyngeal cancers, and multiple census-based factors of SES. Measures of rural-urban status, the percentage of the population that currently smoked, and the percentage of women who reported having ever had a Papanicolaou (Pap) test were also examined as confounders in the association between SES and potentially HPVassociated cancers.…”

Infecção pelo papilomavírus humano: etiopatogenia, biologia molecular e manifestações clínicas

“…45 Clinically, it presents as a plaque, usually single, without tendency to spontaneous regression and with potential to progress to SCC. Some authors consider that mucosal BD corresponds to erythroplasia of Queyrat (EQ).…”