Presence of 5-HT-positive neurons in the medial nuclei of the solitary tract

Calzà, Laura; Giardino, Luciana; Grimaldi, Roberta; Rigolf, Milena; Steinbusch, H.W.M.; M, Tiengo

doi:10.1016/0006-8993(85)90900-x

Cited by 45 publications

(14 citation statements)

References 14 publications

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“…6D). Here VGLUT3 mRNA-expressing cells were exclusively located in the medial subdivision, an area where serotonin-immunoreactive neurons were described previously (53). VGLUT3 mRNA could not be detected in the area postrema.…”

Section: Antibody Specificity and Expression Of Vglut3 On Synapticsupporting

confidence: 56%

Molecular Cloning and Functional Identification of Mouse Vesicular Glutamate Transporter 3 and Its Expression in Subsets of Novel Excitatory Neurons

Schäfer¹,

Varoqui²,

Defamie³

et al. 2002

Journal of Biological Chemistry

372

392

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We have cloned and functionally characterized a third isoform of a vesicular glutamate transporter (VGLUT3) expressed on synaptic vesicles that identifies a distinct glutamatergic system in the brain that is partly and selectively promiscuous with cholinergic and serotoninergic transmission. Transport activity was specific for glutamate, was H ؉ -dependent, was stimulated by Cl ؊ ion, and was inhibited by Rose Bengal and trypan blue. Northern analysis revealed higher mRNA levels in early postnatal development than in adult brain. Restricted patterns of mRNA expression were observed in presumed interneurons in cortex and hippocampus, and projection systems were observed in the lateral and ventrolateral hypothalamic nuclei, limbic system, and brainstem. Double in situ hybridization histochemistry for vesicular acetylcholine transporter identified VGLUT3 neurons in the striatum as cholinergic interneurons, whereas VGLUT3 mRNA and protein were absent from all other cholinergic cell groups. In the brainstem VGLUT3 mRNA was concentrated in mesopontine raphé nuclei. VGLUT3 immunoreactivity was present throughout the brain in a diffuse system of thick and thin beaded varicose fibers much less abundant than, and strictly separated from, VGLUT1 or VGLUT2 synapses. Co-existence of VGLUT3 in VMAT2-positive and tyrosine hydroxylase -negative varicosities only in the cerebral cortex and hippocampus and in subsets of tryptophan hydroxylase-positive cell bodies and processes in differentiating primary raphé neurons in vitro indicates selective and target-specific expression of the glutamatergic/serotoninergic synaptic phenotype.

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Section: Antibody Specificity and Expression Of Vglut3 On Synapticsupporting

confidence: 56%

Molecular Cloning and Functional Identification of Mouse Vesicular Glutamate Transporter 3 and Its Expression in Subsets of Novel Excitatory Neurons

Schäfer¹,

Varoqui²,

Defamie³

et al. 2002

Journal of Biological Chemistry

372

392

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“…If, as seems likely, NTS 5-HT 2 receptors play a facilitatory role in depressor re¯exes, the site of origin of the 5-HT and the conditions under which it is released remains unclear. However, there are several possible sources including intrinsic neurones in the NTS itself (Calza et al, 1985) and the area postrema (Steinbusch, 1981). In addition, projections to the NTS from 5-HT-containing neurones in raphe magnus and nucleus paragigantocellularis at the medullary level and from the midline raphe and dorsal raphe nuclei in the pons.…”

Section: Discussionmentioning

confidence: 99%

In vivo modulation of vagal‐identified dorsal medullary neurones by activation of different 5‐Hydroxytryptamine₂ receptors in rats

Sévoz‐Couche¹,

Spyer²,

Jordan³

2000

British J Pharmacology

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1 In in vivo experiments, DOI (a 5-HT 2 receptor agonist), MK-212 (a 5-HT 2C receptor agonist), and BW-723C86 (a 5-HT 2B receptor agonist) were applied by ionophoresis to neurones in the rat nucleus tractus solitarius (NTS) receiving vagal a erent input. 2 The majority of the putative`monosynaptically' vagal activated cells were inhibited by both MK-212 (4/6) and DOI (2/4), but una ected by . In contrast,`polysynaptically' activated NTS cells were excited by both BW-723C86 (13/19) and DOI (9/10). Inactive`intermediate' cells were inhibited by BW-723C86 (9/12), MK-212 (5/6) and DOI (3/4), whilst active cells of this group were excited by BW-723C86 (7/13) and DOI (5/5). 3 The selective 5-HT 2B receptor antagonist LY-202715 signi®cantly reduced the excitatory actions of BW-723C86 on`intermediate' and`polysynaptic' cells (13/13), but not the inhibitory e ects observed on inactive Group 2 cells (n=5) whereas the selective 5-HT 2C receptor antagonist RS-102221 reversed the inhibitory e ects of MK-212 and DOI on`monosynaptic and`intermediate' neurones. 4 Cardio-pulmonary a erent stimulation inhibited two of four putative`monosynaptically' activated calls and all four inactive intermediate cells. These were also inhibited by DOI and MK-212. In contrast, cardio-pulmonary a erents excited all ®ve active intermediate cells and all six putative`polysynaptically' activated NTS cells, while all were also previously excited by BW-723C86 and/or DOI. 5 In conclusion, these data demonstrate that neurones in the NTS are a ected di erently by 5-HT 2 receptor ligands, in regard of their vagal postsynaptic location, the type of cardio-pulmonary a erent they receive and the di erent 5-HT 2 receptors activated.

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“…In addition, chemical stimulation of the ventral surface increases activity in pulmonary vagal motoneurones (Haxhiu et al, 1987). Further, all these areas are known to contain 5-HTIA binding sites in cats (Dashwood et al, 1988), rats (Thor et al, 1992a, b) and man (Pazos et al, 1987) and 5-HT cell bodies and fibres (Steinbusch, 1981;Calza et al, 1985;Izzo et al, 1988;. Therefore, in the present study, these 5-HTlA receptor ligands could be acting at one or more of the above sites to modulate the reflex activation of pulmonary vagal motoneurones by activation of bronchial and pulmonary C-fibres.…”

Section: Discussionmentioning

confidence: 99%

Involvement of central 5‐HT_1A receptors in the reflex activation of pulmonary vagal motoneurones by inhaled capsaicin in anaesthetized cats

Bootle

Adcock

Ramage

1996

British J Pharmacology

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1 The aim of the present experiments was to determine whether 5-HTIA receptors play a role in the control of the reflex activation of pulmonary vagal motoneurones. This was carried out by investigating the effects of intracisternal injections (i.c.) of the 5-HTIA receptor ligands, 8-OH-DPAT (50 ,ug kg-'), buspirone (200 ug kg-'), WAY-100635 (100 Mg kg-'), methiothepin (200 Mg kg-') and (-)-pindolol (100 Mg kg-') and the 5-HT2 receptor antagonist, cinanserin (200 ug kg-'), on the reflex bronchoconstriction evoked by inhaled capsaicin aerosol in a-chloralose anaesthetized, neuromuscularly blocked and artificially ventilated cats. Recordings were made of heart rate, blood pressure and upper tracheal pressure.2 Central application of all the 5-HTIA receptor antagonists (methiothepin, WAY-100635 and (-)-pindolol) attenuated the reflex bronchoconstriction in the upper trachea. However, the same dose of WAY-100635 given i.v. had no effect on this reflex bronchoconstriction. The 5-HTlA receptor agonist, 8-OH-DPAT (50 Mg kg-') given i.c., potentiated the capsaicin-evoked reflex bronchoconstriction, whereas buspirone (200 Mg kg-') i.c. had no effect. The 5-HT2 receptor antagonist, cinanserin (200 Mg kg-') also had no effect. 3 It is concluded that the reflex excitation of pulmonary vagal motoneurones by inhaled capsaicin in achloralose anaesthetized cats involves the activation of central 5-HTIA receptors.

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Presence of 5-HT-positive neurons in the medial nuclei of the solitary tract

Cited by 45 publications

References 14 publications

Molecular Cloning and Functional Identification of Mouse Vesicular Glutamate Transporter 3 and Its Expression in Subsets of Novel Excitatory Neurons

Molecular Cloning and Functional Identification of Mouse Vesicular Glutamate Transporter 3 and Its Expression in Subsets of Novel Excitatory Neurons

In vivo modulation of vagal‐identified dorsal medullary neurones by activation of different 5‐Hydroxytryptamine₂ receptors in rats

Involvement of central 5‐HT_1A receptors in the reflex activation of pulmonary vagal motoneurones by inhaled capsaicin in anaesthetized cats

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Presence of 5-HT-positive neurons in the medial nuclei of the solitary tract

Cited by 45 publications

References 14 publications

Molecular Cloning and Functional Identification of Mouse Vesicular Glutamate Transporter 3 and Its Expression in Subsets of Novel Excitatory Neurons

Molecular Cloning and Functional Identification of Mouse Vesicular Glutamate Transporter 3 and Its Expression in Subsets of Novel Excitatory Neurons

In vivo modulation of vagal‐identified dorsal medullary neurones by activation of different 5‐Hydroxytryptamine2 receptors in rats

Involvement of central 5‐HT1A receptors in the reflex activation of pulmonary vagal motoneurones by inhaled capsaicin in anaesthetized cats

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In vivo modulation of vagal‐identified dorsal medullary neurones by activation of different 5‐Hydroxytryptamine₂ receptors in rats

Involvement of central 5‐HT_1A receptors in the reflex activation of pulmonary vagal motoneurones by inhaled capsaicin in anaesthetized cats