Presence of a lethal protease in the extracellular products of <i>Vibrio splendidus</i>–<i>Vibrio lentus</i> related strains

Farto, Rosa; Armada, Susana P.; Montes, M.; Pérez, M.C.; Nieto, T. P.

doi:10.1111/j.1365-2761.2006.00746.x

Cited by 13 publications

(11 citation statements)

References 28 publications

(39 reference statements)

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“…Hitherto, V. splendidus has been associated with disease outbreaks in molluscs (Gay, Ranault, Pons & Le Roux 2004; Gómez‐León, Villamil, Lemos, Novoa & Figueras 2005), but most reports from fish deal with hatchery‐reared larvae (Sedano, Zorrilla, Moriñigo, Balebona, Vidaurreta, Bordas & Borrego 1996; Gatesoupe, Lambert & Nicolas 1999; Thomson, Macpherson, Riaza & Birkbeck 2005). Although some V. splendidus strains have been associated with diseased juvenile‐cultured turbot and cod (Angulo, López & Vicente 1994; Santos, Pazos, Núñez & Toranzo 1997), their virulence is variable and recent attempts to experimentally induce mortality with some of them in turbot have failed (Farto, Armada, Montes, Pérez & Nieto 2006).…”

Section: Discussionmentioning

confidence: 99%

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Bacteria associated with winter mortalities in laboratory-reared common dentex (Dentex dentex L.)

et al. 2007

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A pathological study was conducted on laboratoryreared juvenile common dentex (Dentex dentex) suffering trickling and continuous mortalities. During a 3-month period (October^December), water temperature, clinical signs and mortalities were recorded. Moribund or dead ¢sh were examined for bacteria and parasites. Bacteria were isolated from head kidney and external ulcers, and samples from the gills, intestine, stomach, trunk kidney, gall bladder and liver were taken for histology. Cumulative mortality reached 73%, and 80% of ¢sh examined were positive for bacteria (102 isolates).Vibrio splendidus was the most prevalent in head kidney (59.7%) and ulcers (88.9%), and it was frequently isolated as pure culture (74.7% and 100% respectively). This is the ¢rst report of this bacterium in association with mortality in common dentex.Vibrio scophthalmi was the second most prevalent bacterium (29.2%) and accounted for 63.6% of the mixed infections with V. splendidus, V. harveyi, Pseudoalteromonas spp. and other species were rarely isolated. No parasites were found in histological sections. Bacteria were frequently observed in the lumen of the intestine and stomach, destroying the epithelium. A cellular reaction was suggested by the high numbers of rodlet cells (RC) in the intestinal epithelium, and the abundance of eosinophilic granular cells (EGC) in the intestinal lamina propria. In the stomach, vacuolized cells containing unidenti¢ed debris, sometimes of crystalloid appearance, were very common. The high prevalence of V. splendidus in pure cultures and the absence of other aetiological agents suggest that V. splendidus is substantially involved in the registered mortalities, although the implication of V. scophthalmi and even of some nutritional factors cannot be discounted.

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Section: Discussionmentioning

confidence: 99%

“…Experimental infections with the different serotypes of the two species are necessary to reveal their actual virulence. In addition, the possible involvement of lethal proteases, as reported for some V. splendidus ‐related strains (Farto et al 2006), should be explored.…”

Section: Discussionmentioning

confidence: 99%

Bacteria associated with winter mortalities in laboratory-reared common dentex (Dentex dentex L.)

et al. 2007

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“…These enzymes damage the extracellular matrix of animals and thus are important both for tissue colonization and nutrients supply. In a study of the fish pathogen F. columnare , a chondroitin lyase was found to be related to the virulence (Suomalainen et al, 2006). Plant and algal pathogens are able to degrade cell wall compounds such as pectins and agars.…”

Section: The Phylum Bacteroidetesmentioning

confidence: 99%

Environmental and Gut Bacteroidetes: The Food Connection

Thomas¹,

Hehemann²,

Rebuffet³

et al. 2011

Front. Microbio.

1,019

645

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Members of the diverse bacterial phylum Bacteroidetes have colonized virtually all types of habitats on Earth. They are among the major members of the microbiota of animals, especially in the gastrointestinal tract, can act as pathogens and are frequently found in soils, oceans and freshwater. In these contrasting ecological niches, Bacteroidetes are increasingly regarded as specialists for the degradation of high molecular weight organic matter, i.e., proteins and carbohydrates. This review presents the current knowledge on the role and mechanisms of polysaccharide degradation by Bacteroidetes in their respective habitats. The recent sequencing of Bacteroidetes genomes confirms the presence of numerous carbohydrate-active enzymes covering a large spectrum of substrates from plant, algal, and animal origin. Comparative genomics reveal specific Polysaccharide Utilization Loci shared between distantly related members of the phylum, either in environmental or gut-associated species. Moreover, Bacteroidetes genomes appear to be highly plastic and frequently reorganized through genetic rearrangements, gene duplications and lateral gene transfers (LGT), a feature that could have driven their adaptation to distinct ecological niches. Evidence is accumulating that the nature of the diet shapes the composition of the intestinal microbiota. We address the potential links between gut and environmental bacteria through food consumption. LGT can provide gut bacteria with original sets of utensils to degrade otherwise refractory substrates found in the diet. A more complete understanding of the genetic gateways between food-associated environmental species and intestinal microbial communities sheds new light on the origin and evolution of Bacteroidetes as animals’ symbionts. It also raises the question as to how the consumption of increasingly hygienic and processed food deprives our microbiota from useful environmental genes and possibly affects our health.

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“…The presence of a lethal extracellular 39-kDa protease, similar to that of Vibrio pelagius , was detected in 15% of the ECP assayed belonging to strains of the Vibrio splendidus – V. lentus related group by Farto et al (2006), which suggested their potential risk for the health of reared aquatic organisms.…”

Section: New Vibrio Species Associated To Molluscsmentioning

confidence: 99%

New Vibrio species associated to molluscan microbiota: a review

et al. 2014

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The genus Vibrio consists of more than 100 species grouped in 14 clades that are widely distributed in aquatic environments such as estuarine, coastal waters, and sediments. A large number of species of this genus are associated with marine organisms like fish, molluscs and crustaceans, in commensal or pathogenic relations. In the last decade, more than 50 new species have been described in the genus Vibrio, due to the introduction of new molecular techniques in bacterial taxonomy, such as multilocus sequence analysis or fluorescent amplified fragment length polymorphism. On the other hand, the increasing number of environmental studies has contributed to improve the knowledge about the family Vibrionaceae and its phylogeny. Vibrio crassostreae, V. breoganii, V. celticus are some of the new Vibrio species described as forming part of the molluscan microbiota. Some of them have been associated with mortalities of different molluscan species, seriously affecting their culture and causing high losses in hatcheries as well as in natural beds. For other species, ecological importance has been demonstrated being highly abundant in different marine habitats and geographical regions. The present work provides an updated overview of the recently characterized Vibrio species isolated from molluscs. In addition, their pathogenic potential and/or environmental importance is discussed.

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Presence of a lethal protease in the extracellular products of Vibrio splendidus–Vibrio lentus related strains

Cited by 13 publications

References 28 publications

Bacteria associated with winter mortalities in laboratory-reared common dentex (Dentex dentex L.)

Bacteria associated with winter mortalities in laboratory-reared common dentex (Dentex dentex L.)

Environmental and Gut Bacteroidetes: The Food Connection

New Vibrio species associated to molluscan microbiota: a review

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