Presence of acyl‐homoserine lactones in 57 members of the<i>Vibrionaceae</i>family

Purohit, Amit Anand; Johansen, Jostein; Hansen, Hilde; Leiros, Hanna‐Kirsti S.; Kashulin, Alexander; Karlsen, Christian; Smalås, Arne O.; Haugen, Peik; Willassen, Nils Peder

doi:10.1111/jam.12264

Cited by 43 publications

(84 citation statements)

References 66 publications

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“…A cold temperature is favorable for expressing LCD phenotypes and probably increases the ability of the bacteria to colonize a surface. The expression of litR is most likely expressed in response to accumulation of QS signals such as acylated homoserine lactones (AHLs), and several AHLs have been identified in the stationary growth phase of V. salmonicida (58,59). Thus, as the cell density increases, the accumulation of AHLs and the expression of litR downregulates the biofilm, yielding planktonic cells that are able to escape and colonize new sites.…”

Section: Discussionmentioning

confidence: 99%

LitR Is a Repressor of syp Genes and Has a Temperature-Sensitive Regulatory Effect on Biofilm Formation and Colony Morphology in Vibrio (Aliivibrio) salmonicida

Hansen

Bjelland

Ronessen

et al. 2014

Appl Environ Microbiol

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c Vibrio (Aliivibrio) salmonicida is the etiological agent of cold water vibriosis, a disease in farmed Atlantic salmon (Salmo salar) that is kept under control due to an effective vaccine. A seawater temperature below 12°C is normally required for disease development. Quorum sensing (QS) is a cell density-regulated communication system that bacteria use to coordinate activities involved in colonization and pathogenesis, and we have previously shown that inactivation of the QS master regulator LitR attenuates the V. salmonicida strain LFI1238 in a fish model. We show here that strain LFI1238 and a panel of naturally occurring V. salmonicida strains are poor biofilm producers. Inactivation of litR in the LFI1238 strain enhances medium-and temperaturedependent adhesion, rugose colony morphology, and biofilm formation. Chemical treatment and electron microscopy of the biofilm identified an extracellular matrix consisting mainly of a fibrous network, proteins, and polysaccharides. Further, by microarray analysis of planktonic and biofilm cells, we identified a number of genes regulated by LitR and, among these, were homologues of the Vibrio fischeri symbiosis polysaccharide (syp) genes. The syp genes were regulated by LitR in both planktonic and biofilm lifestyle analyses. Disruption of syp genes in the V. salmonicida ⌬litR mutant alleviated adhesion, rugose colony morphology, and biofilm formation. Hence, LitR is a repressor of syp transcription that is necessary for expression of the phenotypes examined. The regulatory effect of LitR on colony morphology and biofilm formation is temperature sensitive and weak or absent at temperatures above the bacterium's upper threshold for pathogenicity.

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Section: Discussionmentioning

confidence: 99%

LitR Is a Repressor of syp Genes and Has a Temperature-Sensitive Regulatory Effect on Biofilm Formation and Colony Morphology in Vibrio (Aliivibrio) salmonicida

Hansen

Bjelland

Ronessen

et al. 2014

Appl Environ Microbiol

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“…The production of AHLs has been described in several marine Gram-negative bacteria (Parsek et al, 1999;Daniels et al, 2004), and the widespread occurrence of QS molecules in marine alphaproteobacteria has been shown for both free-living strains and those associated with eukaryotic algae (Wagner-Döbler et al, 2005). Nevertheless, AHL production has been so far reported chiefly among members of the Vibrionaceae family of Gram-negative marine bacteria (Purohit et al, 2013). Huang and colleagues (2007) showed differences in patterns of AHL biosynthesis either due to temporal regulation of AHL production within biofilms or due to different bacterial species dominating at different stages of biofilm development.…”

Section: Marine Biofilms and Chemical Cuesmentioning

confidence: 99%

Marine biofilms: diversity of communities and of chemical cues

Antunes

Leão

Vasconcelos

2018

Environ Microbiol Rep

103

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Summary Surfaces immersed in seawater are rapidly colonized by various microorganisms, resulting in the formation of heterogenic marine biofilms. These communities are known to influence the settlement of algae spores and invertebrate larvae, triggering a succession of fouling events, with significant environmental and economic impacts. This review covers recent research regarding the differences in composition of biofilms isolated from different artificial surface types and the influence of environmental factors on their formation. One particular phenomenon – bacterial quorum sensing (QS) – allows bacteria to coordinate swarming, biofilm formation among other phenomena. Some other marine biofilm chemical cues are believed to modulate the settlement and the succession of macrofouling organisms, and they are also reviewed here. Finally, since the formation of a marine biofilm is considered to be an initial, QS‐dependent step in the development of marine fouling events, QS inhibition is discussed on its potential as a tool for antibiofouling control in marine settings.

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“…Acyl-HSL signal structure was identified as described previously with modifications (39). The supernatants of three replicate 1-liter batch cultures were collected after the cells were pelleted by centrifugation during the period of peak signal production per cell (day 4 batch culture at an OD 600 of 0.025 to 0.035, according to the bioassay).…”

Section: Methodsmentioning

confidence: 99%

Nitrite-Oxidizing Bacterium Nitrobacter winogradskyi Produces N -Acyl-Homoserine Lactone Autoinducers

Mellbye

Bottomley

Sayavedra-Soto

2015

Appl Environ Microbiol

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bNitrobacter winogradskyi is a chemolithotrophic bacterium that plays a role in the nitrogen cycle by oxidizing nitrite to nitrate. Here, we demonstrate a functional N-acyl-homoserine lactone (acyl-HSL) synthase in this bacterium. The N. winogradskyi genome contains genes encoding a putative acyl-HSL autoinducer synthase (nwi0626, nwiI) and a putative acyl-HSL autoinducer receptor (nwi0627, nwiR) with amino acid sequences 38 to 78% identical to those in Rhodopseudomonas palustris and other Rhizobiales. Expression of nwiI and nwiR correlated with acyl-HSL production during culture. N. winogradskyi produces two distinct acyl-HSLs, N-decanoyl-L-homoserine lactone (C 10 -HSL) and a monounsaturated acyl-HSL (C 10:1 -HSL), in a cell-densityand growth phase-dependent manner, during batch and chemostat culture. The acyl-HSLs were detected by bioassay and identified by ultraperformance liquid chromatography with information-dependent acquisition mass spectrometry (UPLC-IDA-MS). The C‫؍‬C bond in C 10:1 -HSL was confirmed by conversion into bromohydrin and detection by UPLC-IDA-MS.

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Presence of acyl‐homoserine lactones in 57 members of theVibrionaceaefamily

Cited by 43 publications

References 66 publications

LitR Is a Repressor of syp Genes and Has a Temperature-Sensitive Regulatory Effect on Biofilm Formation and Colony Morphology in Vibrio (Aliivibrio) salmonicida

LitR Is a Repressor of syp Genes and Has a Temperature-Sensitive Regulatory Effect on Biofilm Formation and Colony Morphology in Vibrio (Aliivibrio) salmonicida

Marine biofilms: diversity of communities and of chemical cues

Nitrite-Oxidizing Bacterium Nitrobacter winogradskyi Produces N -Acyl-Homoserine Lactone Autoinducers

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