Presence of androgen receptors in the hen hypothalamus and pituitary

Abstract

Abstract. Soluble and insoluble fractions in a hypotonic buffer solution of hypothalamic-preoptic and median eminence areas, and of the anterior pituitary of the hen were found to contain a specific androgen binding component having the properties of a receptor. Administration of testosterone in vivo caused a marked decrease in specific [3H]R1881 (a synthetic androgen) bindings in the soluble fraction with a concomitant increase in the bindings in the insoluble fraction, whereas the sum of the bindings did not change. A similar relationship between the bindings of the two fractions was also observed during an ovulatory cycle. The results may provide an evidence for a direct action of androgens on the hen hypothalamus and pituitary.

Calcitonin Receptor Binding Properties in Bone and Kidney of the Chicken During the Oviposition Cycle

“…(22) The time of decrease in the K d value in the egg-laying hen coincides with the time of increase in the secretion of the ovarian steroid hormones as shown in Table 1. (30,39,40) An in vivo administration of E 2 or P 4 was found to cause the decrease in the K d value (Fig. 5).…”

“…Plasma concentratios of E 2 , P 4 , and T were measured by the use of routine radioimmunoassay methods, (29,30) and ICa of the plasma was determined with a Nova 2 Ionized Calcium Analyzer (Nova Biochemical, Newton, MA, U.S.A.). (31) …”

Comparison of Follicular Estrogen Receptors in Rat, Hamster, and Pig1

“…Cytosolic (soluble fraction in hypotonic buffer solution) and nuclear (insoluble fraction in hypotonic buffer solution) fractions of the large antral follicles or the ovarian remnants of rats and hamsters were prepared by homogenizing in hypotonic TEDB buffer (10 mM Tris-HCI, 1.5 mM EDTA, 1 mM dithiothreitol, pH 7.4) at 4°C according to the method of Kawashima et al [20,21]. The tissues were homogenized in hypotonic TEDB buffer with a Potter-Elvehjem-type glass-teflon homogenizer.…”

“…The specific binding of [ 3 H]E 2 was measured by an exchange procedure using the DCC technique [22], with the modifications reported earlier for estrogen [20] and androgen [21] receptors. The diluent used was TEDB buffer.…”

Progesterone Increases Pituitary VIP Receptor Binding in Hens.

“…A direct action of progesterone on the pituitary of the hen has been demonstrated by the presence of its receptors in cytosolic (KAWASHIMA et al, 1978) and nuclear (KAWASHIMA et al, 1979) fractions of the hen pituitary tissue and by the stimulation of production of LH in isolated pituitary cells in vitro (KAWASHIMA et al, 1982). Besides the progesterone receptor, estrogen KAWASHIMA et al, 1987) and androgen (KAWASHIMA et al, 1989) receptors are also present in the hen pituitary.…”